| Project/Area Number |
25862069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
MASUO YOSUKE 福岡歯科大学, 歯学部, 助教 (40631693)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 口臭産生のメカニズム / β-ガラクトシダーゼ / 口臭 / 口腔内細菌 / 舌苔 / β-galactosidase |
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| Outline of Final Research Achievements |
Volatile sulfur compounds (VSCs) are produced by proteolysis of periodontopathic bacteria. The proteolysis of glycoproteins depends on the initial removal of the carbohydrate side chains, and β-galactosidase in saliva is considered to play a significant role in that reaction. The aim of the present study was to investigate the localization of β-galactosidase and to evaluate the relationship between this enzyme activity and parameters related to oral malodor.β-galactosidase activity was detected in the pellet lysate and whole saliva, but not in the saliva supernatant: the activity level was the same in the pellet lysate and whole saliva. There was a positive correlation between β-galactosidase activity and total protein in the saliva pellet, although total protein was not related to malodor strength.Our results indicate that β-galactosidase is located on the cell surface of oral bacteria and plays an important role in producing the malodor underlying physiological halitosis.
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