Enhancement of the synthesis of EXT2, EXTL3 and CHSY1 by polyamines at the level of translation.
| Project/Area Number |
25870126
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
General physiology
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| Research Institution | Chiba University |
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Principal Investigator |
HIGASHI KYOHEI 千葉大学, 薬学研究科(研究院), 助教 (10463829)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ポリアミン / ヘパラン硫酸 / コンドロイチン硫酸 / 蛋白質合成 / microRNA / プトレッシン / スペルミジン / スペルミン / 翻訳 / mRNA / miRNA |
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| Outline of Final Research Achievements |
We have previously found that polyamines (putrescine, spermidine and spermine) stimulated the expression level of heparan sulfate and 4-O-sulfation of chondroitin sulfate in mammalian cells. For this reason, we looked for glycosaminoglycan biosynthesis genes whose synthesis is enhanced by polyamines at the level of translation. It was found that the synthesis of EXT2, EXTL3 and CHSY1 were enhanced by polyamines at the level of translation. Furthermore, synthesis of EXT2 and CHSY1 were down-regulated by microRNAs at the translation initiation and polyamine inhibited its microRNA function. It was also found that C4ST2 was stimulated by polyamines at the level of transcription. Experiments are in progress to clarify the polyamine stimulation of the synthesis of C4ST2 mRNA.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] The Acute Encephalopathy Induced by Intake of Sugihiratake Mushroom in the Patients with Renal Damage Might Be Associated with the Intoxication of Cyanide and Thiocyanate2015
Author(s)
Akiyama H, Matsuoka H, Okuyama T, Higashi K, Toida T, Komatsu H, Sugita-Konishi Y, Kobori S, Kodama Y, Yoshida M, Endou H.
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Journal Title
Food Safety
Volume: 3
Issue: 1
Pages: 16-29
DOI
NAID
ISSN
2187-8404
Related Report
Peer Reviewed / Open Access
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[Presentation] Effect of polyamines on the synthesis of chondroitin sulfate in mammalian cells2015
Author(s)
Higashi K, Yamaguchi K, Imamura M, Furihata T, Terui Y, Kashiwagi K, Linhardt RJ, Igarashi K, and Toida T.
Organizer
Gordon research conference (Polyamines)
Place of Presentation
Waterville Valley, NH, USA
Year and Date
2015-06-14 – 2015-06-19
Related Report
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[Presentation] Enhancement of the synthesis of EXT2, EXTL3 and CHSY1 by polyamines at the level of translation in mammalian cells.2014
Author(s)
Higashi K, Imamura T, Yamaguchi K, Furihata T, Nishimura K, Kashiwagi K, Linhardt RJ, Igarashi K, and Toida T.
Organizer
Gordon research conference (Proteoglycans)
Place of Presentation
Proctor Academy, NH, USA
Year and Date
2014-07-06 – 2014-07-11
Related Report
Invited
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[Presentation] Enhancement of the synthesis of EXT2, EXTL3 and CHSY1 by polyamines at the level of translation in mammalian cells.2014
Author(s)
Higashi K, Imamura T, Yamaguchi K, Furihata T, Nishimura K, Kashiwagi K, Linhardt RJ, Igarashi K, and Toida T.
Organizer
Glycomics meeting
Place of Presentation
Rensselaer Polytechnic Institute, NY, USA
Year and Date
2014-07-05
Related Report
Invited
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