| Project/Area Number |
25870177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Experimental pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Enomoto Yutaka 東京大学, 分子細胞生物学研究所, 助教 (20608210)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | NK細胞 / IL-4 / IL-15 |
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| Outline of Final Research Achievements |
We examined the phenotype of NK cells in mice over-expressing IL-4 by hydrodynamic tail vein injection. IL-4 expression induced a unique NK cell population, which was different from conventional mature NK cells. Moreover, these IL-4-induced NK cells produced a large amount of cytokines and exhibited a high cytotoxicity compared to conventional mature NK cells. Additionally, IL-4 over-expression was shown to induce proliferation of macrophage, which might contribute to NK cell proliferation.we depleted macrophages by administration of clodronate liposomes and then we overexpressed IL-4. At 3 days after the injection, we analyzed NK cells in the liver by flow cytometry. Depletion of macrophages suppressed the induction of IL4-NK cells in both the liver. Collectively, these results suggest a novel role of IL-4 in Th2 responses through the induction of the cell population with strong NK activity.
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