Lipid analysis of microenvironment in hepatocellular carcinoma using imaging mass spectrometry
Project/Area Number |
25870302
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
Tumor biology
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 大腸癌 / 質量顕微鏡 / CTOS / 癌微小環境 / ホスファチジルイノシトール / 癌周囲微小環境 / 肝細胞癌 |
Outline of Final Research Achievements |
In this study,we aimed to seek out the cancer cell-autonomous acquisition of cancer outer edge-characterizing lipids in colorectal cancer by analysing phospholipids in CTOSs derived from colorectal cancer patients with matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). A signal at m/z 885.5 in negative ion mode was detected specifically at the surface regions. The signal was identified as an arachidonic acid (AA)-containing phosphatidylinositol (PI), PI(18:0/20:4), by tandem mass spectrometry analysis. Quantitative analysis revealed that the amount of PI(18:0/20:4) in the surface region of CTOSs was two-fold higher than that in the medial region. Finally, PI(18:0/20:4) was enriched at the cancer cells/stromal interface in colorectal cancer patients. These data imply a possible importance of AA-containing PI for colorectal cancer progression, and suggest cells expressing AAcontaining PI as potential targets for anti-cancer therapy.
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Report
(5 results)
Research Products
(4 results)
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[Journal Article] Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer.2016
Author(s)
Hiraide T, Ikegami K, Sakaguchi T, Morita Y, Hayasaka T, Masaki N, Waki M, Sugiyama E, Shinriki S, Takeda M, Shibasaki Y, Miyazaki S, Kikuchi H, Okuyama H, Inoue M, Setou M, Konno H.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 29935-29935
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.2013
Author(s)
Morita Y, Sakaguchi T, Ikegami K, Goto-Inoue N, Hayasaka T, Hang VT, Tanaka H, Harada T, Shibasaki Y, Suzuki A, Fukumoto K, Inaba K, Murakami M, Setou M, Konno H.
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Journal Title
J Hepatol.
Volume: 29
Issue: 2
Pages: 292-299
DOI
Related Report
Peer Reviewed
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