Classification and characterization of a novel basal-like breast cancer group.
| Project/Area Number |
25870384
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Tumor biology
Tumor diagnostics
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ITOU Junji 京都大学, 医学(系)研究科(研究院), 特定助教 (10638844)
|
|---|
| Research Collaborator |
MATSUMOTO Yoshiaki
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 癌細胞 / 転写因子 / 増殖能 / 運動能 / Basal-like乳癌 / 幹細胞転写因子 / 細胞増殖 / 細胞運動 / 細胞 / 移動 / 増殖 / 幹細胞 / 細胞生物学 / 分子生物学 / 遺伝学 |
|---|
| Outline of Final Research Achievements |
Understanding molecular characteristics of cancer will facilitate cancer therapy. This study found a novel basal-like breast cancer cell group, the malignancy of which is regulated by a transcription factor SALL4. Knockdown experiments for SALL4 revealed that SALL4 maintains cell proliferation. In addition, SALL4 increases motility via suppression of cell-cell adhesion. These observations contribute to our understanding of basal-like breast cancer.
To further study for basal-like breast cancer cells, this study established a novel fluorescent protein-based proliferation assay. This assay utilizes photoconvertible fluorescent protein, Kaede, for pulse-labeling. In our observation, there always are few non- or slow-cycling cells in basal-like breast cancer cell lines. This technique will be a useful tool for cancer researches and anti-cancer drug development.
|
Report
(3 results)
Research Products
(6 results)
