Project/Area Number |
25870449
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Endocrinology
Orthopaedic surgery
|
Research Institution | Shimane University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | オステオカルシン / 脂肪細胞 / C/EBPβ / PPARγ / 筋芽細胞 |
Outline of Final Research Achievements |
The expressions of osteocalcin and its receptor, GPRC6A, were found in preadipocytic 3T3-L1 cells. Osteocalcin expression was decreased during the differentiation of 3T3-L1 cells, while GPRC6A expression was increased. Although osteocalcin decreased the expression of C/EBPβ and PPARγ, carboxylated and uncarboxylated osteocalcin did not affect the size of adipocytes by Oil red O staining. On the other hand, both osteocalcin inhibited the proliferation of 3T3-L1 cells, suggesting that osteocalcin may suppress the accumulation of fat mass.
|