| Project/Area Number |
25870449
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
Orthopaedic surgery
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| Research Institution | Shimane University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | オステオカルシン / 脂肪細胞 / C/EBPβ / PPARγ / 筋芽細胞 |
|---|
| Outline of Final Research Achievements |
The expressions of osteocalcin and its receptor, GPRC6A, were found in preadipocytic 3T3-L1 cells. Osteocalcin expression was decreased during the differentiation of 3T3-L1 cells, while GPRC6A expression was increased. Although osteocalcin decreased the expression of C/EBPβ and PPARγ, carboxylated and uncarboxylated osteocalcin did not affect the size of adipocytes by Oil red O staining. On the other hand, both osteocalcin inhibited the proliferation of 3T3-L1 cells, suggesting that osteocalcin may suppress the accumulation of fat mass.
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