Fluorescence derivatization method for sensitive detection of glucuronide

Research Project

Project/Area Number	25870670
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Legal medicine Environmental and hygienic pharmacy
Research Institution	Kagoshima University (2014) International University of Health and Welfare (2013)
Principal Investigator	NAKAMAE Takuma 鹿児島大学, 医歯(薬)学総合研究科, 助教 (20453409)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	蛍光誘導体化 / グルクロン酸抱合体 / HPLC / HPLC分析 / DMEQ-H / DMT-MM
Outline of Final Research Achievements	A fluoresence derivatization method of glucuronides using DMEQ-hydrazide and DMT-MM was developed. Ethyl glucuronide, oxazepam glucuronide and propofol glucuronide were derivazed under mild condition and detected by HPLC fluoresence analysis.

Report

(3 results)