Analysis of a polyploid bacterial DNA replication initiation for building of a chromosome copy number control system.
Project/Area Number |
25870714
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Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
Genome biology
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Research Institution | Keio University |
Principal Investigator |
OHTANI Naoto 慶應義塾大学, 政策・メディア研究科, 講師 (70365451)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | 高度好熱菌 / 倍数性 / DNA複製 / メガプラスミド / Thermus thermophilus / 染色体 / 倍数体 |
Outline of Final Research Achievements |
DNA replication initiation of an extremely thermophilic bacterium harboring multiple chromosomal copies in a cell was studied, aiming to build a chromosome copy number control system in bacteria. To identify an essential region for replication initiation on the chromosome, screening for a genomic DNA clone exhibiting autonomous replication ability was performed and the abilities of larger regions copied out of the chromosome were also examined. However, the essential region for replication initiation has not yet been identified. In this study, we have unveiled that the essential part for megaplasmid pTT27 replication consists of two regions positioned distantly on the plasmid. It implies that chromosomal replication initiation might also require distantly-positioned multiple regions on the chromosome. In this case, we must try different approaches to identify the essential region for chromosomal replication initiation.
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Report
(3 results)
Research Products
(3 results)