Influence on the progression of periodontal disease in the suppression of hemolytic activity in P. intermedia

• Project/Area Number: 25870735
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology; Pathobiological dentistry/Dental radiology
• Research Institution: Showa University
• Principal Investigator: SUZUKI Naoko 昭和大学, 歯学部, 兼任講師 (60634855)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Prevotella intermedia / 溶血素遺伝子 / 部位特異的変異酵素 / 溶血活性 / 組み換えタンパク質

Outline of Final Research Achievements

In order to clarify the involvement of hemolysins pathogenicity, firstly we tried to construct P. intermedia hemolysin deficient mutant. However, we could not construct hemolysin deficient mutant. Next, to identify the essential amino acids in the hemolytic activity of HlyA, we tried to construct point mutant enzyme. Replacement of T28, K30, R52, R59, R141, R184, R196, R250, T287, R303, with Gly resulted in complete disappearance of hemolytic activity, implying that these amino acids are essential for this enzyme activity.