| Project/Area Number |
25871077
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
Molecular biology
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
TAKIZAWA Naoki 公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (50448502)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | インフルエンザウイルス / RNAウイルス / 分節化ゲノム / ゲノムパッケージング / ウイルスゲノム / RNAゲノム |
|---|
| Outline of Final Research Achievements |
The influenza A genome consists of eight single-stranded negative-sense viral RNA (vRNA) segments. Previous studies have suggested that each infectious virion must contain at least one set of eight vRNP segments. However, how influenza virions selectively incorporate a set of the eight distinct vRNAs is not known. In this study, we try to identify the RNA motif for packaging of viral genome. As a result, we identified candidate nucleotides important for genome packaging in protein cording region. The virus which has a mutation in the identified nucleotide reduce viral RNAs in culture supernatant from infected cells but the replication and transcription are comparable to those in wild type virus infected cells. These results suggest that the identified nucleotides are important for genome packaging in influenza virion.
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