Differential proteome analysis identifies TGF-beta related pro-metastatic proteins in a 4T1 murine breast cancer model
Project/Area Number |
25871241
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Tumor biology
Genome biology
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Research Institution | Osaka City University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | TGF-beta / Proteomics / eIF signaling / metastatic mouse model / Breast cancer / プロテオミクス / 転移性乳がん / マウスモデル / 乳がん転移促進因子 / TGF-beta阻害剤 / 転移性乳癌モデル / 乳癌転移促進因子 |
Outline of Final Research Achievements |
Although TGF-β antagonists have been proposed as anti-metastatic therapies for patients with advanced stage cancer, how TGF-β mediates metastasis-promoting effects is poorly understood. In the present study, we found that systemic administration of the TGF-β receptor kinase inhibitor, SB-431542, significantly inhibited lung metastasis from transplanted 4T1 mammary tumors in Balb/c mice. The differentially expressed proteins in the comparison of lung metastases from SB-431542 treated and control vehicle-treated groups were analyzed by proteomics analysis. Our proteomic approach newly identified eIF pathway proteins as novel potential mediators of TGF-β tumor-promoting activity.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Differential Proteome Analysis Identifies TGF-beta-Related Pro-Metastatic Proteins in a 4T1 Murine Breast Cancer Model.2015
Author(s)
Sato, M., Matsubara, T., Adachi, J., Hashimoto, Y., Fukamizu, K., Kishida, M., Yang, Y.A., Wakefield, L.M., and Tomonaga, T
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Journal Title
PloS One
Volume: 10
Issue: 5
Pages: e0126483-e0126483
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Definition of Smad3 phosphorylation events that affect malignant and metastatic behaviors in breast cancer cells.2014
Author(s)
Bae E, Sato M, Kim RJ, Kwak MK, Naka K, Gim J, Kadota M, Tang B, Flanders KC, Kim TA, Leem SH, Park T, Liu F, Wakefield LM, Kim SJ, Ooshima A.
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Journal Title
Cancer Res.
Volume: 74
Pages: 6139-6149
Related Report
Peer Reviewed / Open Access
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[Journal Article] An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-β in human breast cancer.2014
Author(s)
Sato M, Kadota M, Tang B, Yang HH, Yang Y, Shan M, Weng J, Welsh MA, Flanders KC, Nagano Y, Michalowski AM, Clifford RJ, Lee MP, Wakefield LM.
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Journal Title
Breast Cancer Research
Volume: 16
Issue: 3
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A novel mechanism of keratin cytoskeleton organization through casein kinase I alpha and FAM83H in colorectal cancer.2013
Author(s)
Kuga, T., Kume, H., Kawasaki, N., Sato, M., Adachi, J., Shiromizu, T., Hoshino, I., Nishimori, T., Matsubara, H., and Tomonaga T.
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Journal Title
J Cell Sci
Volume: 126
Pages: 4721-31
DOI
Related Report
Peer Reviewed
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