Functional analyses of PATE4 in sperm maturation using genetically modified mice

• Project/Area Number: 25890013
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: NODA Taichi 大阪大学, 微生物病研究所, 特任研究員(常勤) (50712551)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 精嚢腺 / 副生殖腺 / 精子成熟 / 精巣上体 / 遺伝子改変マウス

Outline of Final Research Achievements

In general, cauda epididymal sperm are used for in vitro fertilization. However, in vivo, seminal vesicle secretions (SVSs) are attached to epididymal sperm when those sperm are ejaculated. To reveal the function of SVSs in vivo, male mice removed the seminal vesicle were made. Those males led to the decrease in male reproductive performance, suggesting the importance of the seminal vesicle in male fertility. Next, the function of an SVS called PATE4 (prostate and testis expression 4) was analyzed using genetically modified mice. Pate4 KO mice were created with a gene targeting method and then WT females cohabited with Pate4 KO males barely delivered newborns, indicating that Pate4 KO males tended towards subfertility. The cause of the phenotype in the Pate4 KO male will be analyzed in detail.

Research Products

• [Journal Article] Roles of extracellular Ca2+ in the occurrence of full-type hyperactivation in boar ejaculated spermatozoa pre-incubated to induce the cAMP-triggered events (2015)
  • Author(s): Aya Kojima, Yuki Matsushita, Yukari Ogura, Sho Ishikawa, Taichi Noda, Tetsuma Murase and Hiroshi Harayama
  • Journal Title: Andrology Volume: 3 Issue: 2 Pages: 321-331
  • DOI: 10.1111/andr.12005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distinct segment-specific functions of calyculin A-sensitive protein phosphatases in the regulation of cAMP-triggered events in ejaculated bull spermatozoa (2015)
  • Author(s): Yohei Mizuno, Ayane Isono, Aya Kojima, Miyuki M. Arai, Taichi Noda, Mitsuhiro Sakase, Moriyuki Fukushima and Hiroshi Harayama
  • Journal Title: Molecular Reproduction and Development Volume: 82 Issue: 3 Pages: 232-250
  • DOI: 10.1002/mrd.22465
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 哺乳類におけるゲノム編集の現状と今後の展望 (2015)
  • Author(s): 野田大地，伊川正人
  • Journal Title: 脳21, 18巻, 1号 Volume: 18 Pages: 104-109
• [Journal Article] Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes (2014)
  • Author(s): Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
  • Journal Title: Development, Growth & Differentiation Volume: 56 Issue: 1 Pages: 122-129
  • DOI: 10.1111/dgd.12113
  • Peer Reviewed
• [Journal Article] Expression patterns of the activator type of cAMP-responsive element modulator in testicular germ cells of Japanese Black bulls (2014)
  • Author(s): Taichi Noda, Kenta Minami, Aya Kojima, Yohei Mizuno, Ayane Isono, Mitsuhiro Sakase, Moriyuki Fukushima, Hiroshi Harayama
  • Journal Title: Theriogenology Volume: 81 Issue: 8 Pages: 1012-1020
  • DOI: 10.1016/j.theriogenology.2014.01.014
  • Peer Reviewed
• [Presentation] ウシ精巣におけるcAMP依存性転写調節因子CREMの発現パターンの解析 (2014)
  • Author(s): 野田 大地，南 健太，坂瀬 充洋，福島 護之，原山 洋
  • Organizer: 日本繁殖生物学会大会 帯広大会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2014-08-21 – 2014-08-24
• [Remarks] 大阪大学 研究者総覧
  • URL: http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=10000987&sm=affiliation&sl=ja&sp=2
• [Remarks] Google Scholar
  • URL: https://scholar.google.co.jp/citations?user=6tGVU2EAAAAJ&hl=ja
• [Remarks] 大阪大学 研究者総覧
  • URL: http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=10000987&sm=affiliation&sl=ja&sp=2
• [Remarks] 大阪大学 微生物病研究所 遺伝子機能解析分野 研究者紹介ページ
  • URL: http://www.egr.biken.osaka-u.ac.jp/information/taichi_noda.html
• [Remarks] Google scholar
  • URL: http://scholar.google.co.jp/citations?user=6tGVU2EAAAAJ&hl=ja