New insight into the function of the guard cell chloroplast by a genetic approach

• Project/Area Number: 25891020
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: NEGI JUNTARO 九州大学, 理学(系)研究科(研究院), 特任助教 (70529099)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 気孔 / 葉緑体 / CO2 / 環境応答 / シロイヌナズナ / 孔辺細胞 / オルガネラ

Outline of Final Research Achievements

Guard cell chloroplasts have been proposed to play an important role in the osmoregulatory mechanisms mediating stomatal movements, although their function has been a subject of debate and remains to be confirmed. In this study, I isolated an Arabidopsis gles1 mutant that had non-chlorophyllous stomata. CO2 and light dependent stomatal responses were severely disturbed in the non-chlorophyllous stomata of the gles1 mutant. Whole-cell patch-clamp experiments indicated that in the mutant guard cells, CO2 activation of the S-type anion channels was impaired. These results provide direct evidence to support the idea that guard cell chloroplasts are essential for light-induced stomatal opening and CO2-induced stomatal closure.

Research Products

• [Journal Article] Natural variation in stomatal responses to environmental changes among Arabidopsis thaliana ecotypes. (2015)
  • Author(s): Sho Takahashi*, Keina Monda* (*co-first author), Juntaro Negi, Fumitaka Konishi, Shinobu Ishikawa, Mimi Hashimoto-Sugimoto, Nobuharu Goto, Koh Iba
  • Journal Title: PLOS ONE Volume: 10(2) Issue: 2 Pages: e0117449-e0117449
  • DOI: 10.1371/journal.pone.0117449
  • Peer Reviewed / Open Access
• [Journal Article] New Approaches to the Biology of Stomatal Guard Cells. (2014)
  • Author(s): Negi, J., Hashimoto-Sugimoto, M., Kusumi, K. and Iba, K.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 2 Pages: 241-250
  • DOI: 10.1093/pcp/pct145
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Dof Transcription Factor, SCAP1, Is Essential for the Development of Functional Stomata in Arabidopsis (2013)
  • Author(s): Negi, J., Moriwaki, K., Konishi, M., Yokoyama, R., Nakano, T., Kusumi, K., Hashimoto-Sugimoto, M., Schroeder, J.I., Nishitani, K., Yanagisawa, S. and Iba, K.
  • Journal Title: Current Biology Volume: 23 Issue: 6 Pages: 479-484
  • DOI: 10.1016/j.cub.2013.02.001
  • Peer Reviewed
• [Journal Article] A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses (2013)
  • Author(s): Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K.
  • Journal Title: Nature Commun. Volume: 4 Issue: 1 Pages: 2215-2215
  • DOI: 10.1038/ncomms3215
  • Peer Reviewed / Open Access
• [Journal Article] An S-Type Anion Channel SLAC1 Is Involved in Cryptogein-Induced Ion Fluxes and Modulates Hypersensitive Responses in Tobacco BY-2 Cells (2013)
  • Author(s): Kurusu, T., Saito, K., Horikoshi, S., Hanamata, S., Negi, J., Yagi, C., Kitahata, N., Iba, K. and Kuchitsu, K.
  • Journal Title: PLOS One Volume: 8 Issue: 8 Pages: e70623-e70623
  • DOI: 10.1371/journal.pone.0070623
  • Peer Reviewed / Open Access
• [Journal Article] 気孔のCO2応答 (2013)
  • Author(s): 橋本(杉本) 美海、祢宜 淳太郎、楠見 健介、射場 厚
  • Journal Title: 化学と生物 Volume: 51 Pages: 831-839
• [Presentation] Isolation and characterization of the suppressor mutants of ht1-2 in Arabidopsis (2015)
  • Author(s): Sakiko Saito, Juntaro Negi, Keina Monda, Mikiko Kojima, Hitoshi Sakakibara, Koh Iba
  • Organizer: 26th International Conference on Aabidopsis Research (ICAR)
  • Place of Presentation: Paris
  • Year and Date: 2015-07-05 – 2015-07-09
• [Presentation] Transmembrane region of the Arabidopsis guard cell SLAC1 anion channel involved in stomatal CO2 response (2015)
  • Author(s): Yoshiko Yamamoto, Juntaro Negi, Cun Wang, Julian Schroeder, Koh Iba
  • Organizer: 26th International Conference on Aabidopsis Research (ICAR)
  • Place of Presentation: Paris
  • Year and Date: 2015-07-05 – 2015-07-09
• [Presentation] CO2 activation of the guard cell S-type anion channel is impaired in an Arabidopsis mutant with non-chlorophyllous stomata (2015)
  • Author(s): Juntaro Negi, Kensuke Kusumi, Shintaro Munemasa, Mayumi Fujita, Julian Schroeder, Koh Iba
  • Organizer: 26th International Conference on Aabidopsis Research (ICAR)
  • Place of Presentation: Paris
  • Year and Date: 2015-07-05 – 2015-07-09
• [Presentation] 気孔機能化に関わるSCAP1遺伝子のプロモーター解析 (2015)
  • Author(s): 森脇 宏介, 佐竹 秀元, 祢宜 淳太郎，柳澤 修一, 射場 厚
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京都（東京農大世田谷キャンパス）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 新規CO2非感受性変異体cdi4の解析 (2014)
  • Author(s): 岡部 誠, 祢宜 淳太郎, 中野 利彬, 田畑 亮, 山口 勝司, 重信 秀治, 山田 昌史, 長谷部 光泰, 澤 進一郎, 射場 厚
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 神奈川県（明治大学生田キャンパス）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 気孔閉鎖を司る陰イオンチャネルSLAC1のCO2シグナル受容部位はABAシグナル受容部位とは異なる (2014)
  • Author(s): 山本 禎子, 祢宜 淳太郎 , Wang Cun, Schroeder Julian, 磯貝 泰弘, 射場 厚
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 神奈川県（明治大学生田キャンパス）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 順遺伝子学的手法を用いた孔辺細胞特異的葉緑体機能の解析 (2014)
  • Author(s): 祢宜 淳太郎、楠見 健介、 宗正 晋太郎、藤田 麻友美、Julian Schroeder、射場 厚
  • Organizer: 第55回日本植物生理学会
  • Place of Presentation: 富山
  • Year and Date: 2014-03-18 – 2014-03-20
• [Presentation] 気孔にガス交換機能を付与する鍵転写因子SCAP1 (2013)
  • Author(s): 祢宜 淳太郎、森脇 宏介、小西 美稲子、横山 隆亮、中野 利彬、 楠見 健介、橋本 美海、西谷 和彦、柳澤 修一、射場 厚
  • Organizer: 第31回植物分子細胞生物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-10 – 2013-09-12
• [Presentation] 孔辺細胞局在型陰イオンチャネルSLAC1のCO2による活性化制御 (2013)
  • Author(s): 山本 禎子、祢宜 淳太郎、磯貝 泰弘、射場 厚
  • Organizer: 第31回植物分子細胞生物学会
  • Place of Presentation: 札幌
  • Year and Date: 2013-09-10 – 2013-09-12
• [Remarks]
  • URL: http://plant.biology.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://plant.biology.kyushu-u.ac.jp/Index.html