New insight into the function of the guard cell chloroplast by a genetic approach
Project/Area Number |
25891020
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Kyushu University |
Principal Investigator |
NEGI JUNTARO 九州大学, 理学(系)研究科(研究院), 特任助教 (70529099)
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Project Period (FY) |
2013-08-30 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 気孔 / 葉緑体 / CO2 / 環境応答 / シロイヌナズナ / 孔辺細胞 / オルガネラ |
Outline of Final Research Achievements |
Guard cell chloroplasts have been proposed to play an important role in the osmoregulatory mechanisms mediating stomatal movements, although their function has been a subject of debate and remains to be confirmed. In this study, I isolated an Arabidopsis gles1 mutant that had non-chlorophyllous stomata. CO2 and light dependent stomatal responses were severely disturbed in the non-chlorophyllous stomata of the gles1 mutant. Whole-cell patch-clamp experiments indicated that in the mutant guard cells, CO2 activation of the S-type anion channels was impaired. These results provide direct evidence to support the idea that guard cell chloroplasts are essential for light-induced stomatal opening and CO2-induced stomatal closure.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] A Dof Transcription Factor, SCAP1, Is Essential for the Development of Functional Stomata in Arabidopsis2013
Author(s)
Negi, J., Moriwaki, K., Konishi, M., Yokoyama, R., Nakano, T., Kusumi, K., Hashimoto-Sugimoto, M., Schroeder, J.I., Nishitani, K., Yanagisawa, S. and Iba, K.
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Journal Title
Current Biology
Volume: 23
Issue: 6
Pages: 479-484
DOI
Related Report
Peer Reviewed
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[Journal Article] A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses2013
Author(s)
Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K.
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Journal Title
Nature Commun.
Volume: 4
Issue: 1
Pages: 2215-2215
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] An S-Type Anion Channel SLAC1 Is Involved in Cryptogein-Induced Ion Fluxes and Modulates Hypersensitive Responses in Tobacco BY-2 Cells2013
Author(s)
Kurusu, T., Saito, K., Horikoshi, S., Hanamata, S., Negi, J., Yagi, C., Kitahata, N., Iba, K. and Kuchitsu, K.
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Journal Title
PLOS One
Volume: 8
Issue: 8
Pages: e70623-e70623
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 新規CO2非感受性変異体cdi4の解析2014
Author(s)
岡部 誠, 祢宜 淳太郎, 中野 利彬, 田畑 亮, 山口 勝司, 重信 秀治, 山田 昌史, 長谷部 光泰, 澤 進一郎, 射場 厚
Organizer
日本植物学会第78回大会
Place of Presentation
神奈川県(明治大学生田キャンパス)
Year and Date
2014-09-12 – 2014-09-14
Related Report
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