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Molecular mechanisms underlying antibody-mediated inhibition of virus budding

Research Project

Project/Area Number 25892002
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Veterinary medical science
Research InstitutionHokkaido University

Principal Investigator

KAJIHARA Masahiro  北海道大学, 人獣共通感染症リサーチセンター, 研究員 (70711894)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsフィロウイルス / エボラウイルス / マールブルグウイルス / 出芽 / 抗体 / 表面糖蛋白質 / エボラウイルス病 / マールブルグ熱 / エボラ出血熱
Outline of Final Research Achievements

Previously, we reported antibody-mediated inhibition of virus budding from infected cells. However, the molecular basis and contribution to protective immunity of antibody-mediated budding inhibition are yet unknown. We found that antibodies induced by nasal immunization had inhibitory activity of influenza virus release from infected cells. It was morphologically suggested that the progeny virions were deposited on the infected cell surface by the tethering mediated by IgA antibody binding, which may be the molecular basis of cross-protective immunity induced by nasal immunization. We examined the involvement of budding inhibition antibodies in protective immunity through passive immunization of hamsters against Marburg virus infection. Immunization with budding inhibition antibody slightly improved clinical outcomes of infected hamsters, indicating that budding inhibition antibodies are also involved in protective immunity.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (28 results)

All 2015 2014 2013

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) Book (3 results)

  • [Journal Article] The Interaction between TIM-1 and NPC1 is important for the cellular entry of Ebola virus2015

    • Author(s)
      Kuroda M, Fujikura D, Nanbo A, Marzi A, Noyori O, Kajihara M, Maruyama J, Matsuno K, Miyamoto H, Yoshida R, Feldmann H, Takada A
    • Journal Title

      J. Virol.

      Volume: 印刷中 Issue: 12 Pages: 6481-6493

    • DOI

      10.1128/jvi.03156-14

    • NAID

      120005621883

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Seroepidemiological prevalence of multiple species of filoviruses in fruit bats (Eidolon helvum) migrating in Africa.2015

    • Author(s)
      Hirohito Ogawa, Hiroko Miyamoto, Eri Nakayama, Reiko Yoshida, Ichiro Nakamura, Hirofumi Sawa, Akihiro Ishii, Yuka Thomas, Emiko Nakagawa, Keita Matsuno, Masahiro Kajihara, Junki Maruyama, Manabu Igarashi, Kimihito Ito, Ayato Takada et al
    • Journal Title

      Journal of Infectious Diseases

      Volume: jiv063 Issue: suppl 2 Pages: S101-S108

    • DOI

      10.1093/infdis/jiv063

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pathological and molecular diagnosis of the 2013 African swine fever outbreak in Lusaka, Zambia2015

    • Author(s)
      Yabe J, Hamambulu P, Simulundu E, Ogawa H, Kajihara M, Mori-Kajihara A, Changula-Chitanga K, Mwase M, Mweemba-Muwowo M, Chambaro HM, Mataa L, Hang'ombe B, Namangala B, Fandamu P, Sawa H, Takada A, Higashi H, Mweene AS.
    • Journal Title

      Trop Anim Health Prod

      Volume: 47 Issue: 2 Pages: 459-463

    • DOI

      10.1007/s11250-014-0732-0

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive molecular detection of tick-borne phleboviruses leads to the retrospective identification of taxonomically unassigned bunyaviruses and the discovery of a novel member of the genus phlebovirus2015

    • Author(s)
      Matsuno K, Weisend C, Kajihara M, Matysiak C, Williamson BN, Simuunza M, Mweene AS, Takada A, Tesh RB, Ebihara H
    • Journal Title

      Journal of Virology

      Volume: 89 Issue: 1 Pages: 594-604

    • DOI

      10.1128/jvi.02704-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Host Cell Factors Involved in Filovirus Infection2014

    • Author(s)
      Masahiro Kajihara, Ayato Takada
    • Journal Title

      Curr. Trop. Med. Rep.

      Volume: 2 Issue: 1 Pages: 30-40

    • DOI

      10.1007/s40475-015-0039-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection.2014

    • Author(s)
      Makoto Kuroda, Daisuke Fujikura, Osamu Noyori, Masahiro Kajihara, Junki Maruyama, Hiroko Miyamoto, Reiko Yoshida, Ayato Takada.
    • Journal Title

      Biochemical and biophysical research communications

      Volume: 455 Issue: 3-4 Pages: 223-228

    • DOI

      10.1016/j.bbrc.2014.10.144

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ebola and Marburg virus diseases in Africa: Increased risk of outbreaks in previously unaffected areas?2014

    • Author(s)
      Changula K, Kajihara M, Mweene AS, Takada A
    • Journal Title

      Microbiol. Immunol.

      Volume: 58 Issue: 9 Pages: 483-491

    • DOI

      10.1111/1348-0421.12181

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The zoonotic potential of avian influenza viruses isolated from wild waterfowl in Zambia2014

    • Author(s)
      Simulundu E, Nao N, Yabe J, Muto NA, Sithebe T, Sawa H, Manzoor R, Kajihara M, Muramatsu M, Ishii A, Ogawa H, Mweene AS, Takada A
    • Journal Title

      Archives of Virology

      Volume: 159 Issue: 10 Pages: 2633-2640

    • DOI

      10.1007/s00705-014-2124-1

    • NAID

      120005656330

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein.2013

    • Author(s)
      Changula Katendi
    • Journal Title

      Virus Res.

      Volume: 176 Issue: 1-2 Pages: 83-90

    • DOI

      10.1016/j.virusres.2013.05.004

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterosubtypic antiviral activity of hemagglutinin-specific antibodies induced by intranasal immunization with inactivated influenza viruses in mice.2013

    • Author(s)
      Muramatsu M. et al.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e71534-e71534

    • DOI

      10.1371/journal.pone.0071534

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential potential for envelope gycoprotein-mediated steric shielding of host cell surface proteins among filoviruses2013

    • Author(s)
      Noyori Osamu
    • Journal Title

      Virology

      Volume: 446 Issue: 1-2 Pages: 152-161

    • DOI

      10.1016/j.virol.2013.07.029

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the envelope glycoprotein of a novel filovirus, Lloviu virus2013

    • Author(s)
      Maruyama J, Miyamoto H, Kajihara M, Ogawa H, Maeda K, Sakoda Y, Yoshida R, Takada A
    • Journal Title

      J. Virol.

      Volume: 88 Issue: 1 Pages: 99-109

    • DOI

      10.1128/jvi.02265-13

    • NAID

      120005451570

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of Antiviral Activity between IgA and IgG Specific to Influenza Virus Hemagglutinin: Increased Potential of IgA for Heterosubtypic Immunity2013

    • Author(s)
      Muramatsu M, Yoshida R, Yokoyama A, Miyamoto H, Kajihara M, Maruyama J, Nao N, Manzoor R, Takada A
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 1 Pages: e85582-e85582

    • DOI

      10.1371/journal.pone.0085582

    • NAID

      120005425353

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heat Shock Protein 70 Modulates Influenza A Virus Polymerase Activity2013

    • Author(s)
      Manzoor R, Kuroda K, Yoshida R, Tsuda Y, Fujikura D, Miyamoto H, Kajihara M, Kida H, Takada A
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 11 Pages: 7599-7614

    • DOI

      10.1074/jbc.m113.507798

    • NAID

      120005460233

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] A 型インフルエンザウイルスヘマグルチニン特異的IgA 抗体の亜型間交差抗ウイルス活性2014

    • Author(s)
      村松美笑子、吉田玲子、横山文香、宮本洋子、梶原将大、丸山隼輝、直享則、 Rashid Manzoor、高田礼人
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜会議センター(横浜市)
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] フィロウイルスの細胞侵入におけるTIM 1 とNPC1 相互作用の解析2014

    • Author(s)
      黒田誠、藤倉大輔、南保明日香、野依修、梶原将大、丸山隼輝、宮本洋子、 吉田玲子、高田礼人
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜会議センター(横浜市)
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Neutralizing Monoclonal Antibody therapy for H5N1 Highly Pathogenic Avian Influenza virus infection in Animal Models2014

    • Author(s)
      Muramatsu M, Yoshida R, Yokoyama A, Miyamoto H, Kajihara M, Maruyama J, Nao N, Manzoor R, Takada A
    • Organizer
      The 13th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Nara Prefectural New Public Hall (Nara)
    • Year and Date
      2014-09-23 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Correlates of Hsp70 kinetics and influenza A virus polymerase activity2014

    • Author(s)
      Manzoor R, Kuroda K, Yoshida R, Tsuda Y, Fujikura D, Miyamoto H, Kajihara M, Kida H, Takada A
    • Organizer
      The 13th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Nara Prefectural New Public Hall (Nara)
    • Year and Date
      2014-09-23 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 北海道産マダニからの新規フレボウイルスの検出2014

    • Author(s)
      中尾亮、梶原将大、邱永晋、森亜紀奈、直亨則、村松美笑子、好井健太朗、苅和宏明、澤洋文、杉本千尋、高田礼人
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detection and isolation of novel phleboviruses from ticks in Japan2014

    • Author(s)
      Nakao R, Kajihara M, Matsuno K, Qiu Y, Mori A, Nao N, Yoshii K, Kariwa H, Sawa H, Sugimoto C, Takada A, Ebihara H
    • Organizer
      VIII International Conference on Ticks and Tick-borne Pathogens
    • Place of Presentation
      Cape Town (South Africa)
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of the envelope glycoprotein of a novel filovirus, Lloviu virus2014

    • Author(s)
      Maruyama J, Miyamoto H, Kajihara M, Ogawa H, Maeda K, Sakoda Y, Yoshida R, Takada A
    • Organizer
      XVI International Congress of Virology
    • Place of Presentation
      Montreal (Canada)
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] A polymorphism in TIM‐1 is associated with cell susceptibility to filovirus infection2014

    • Author(s)
      Kuroda M, Fujikura D, Noyori O, Nakayama E, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
    • Organizer
      XVI International Congress of Virology
    • Place of Presentation
      Montreal (Canada)
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Different pathogenicity of H5N1 highly pathogenic avian influenza viruses determined by a single amino acid in the M1 protein2014

    • Author(s)
      Nao N, Kajihara M, Maruyama J, Kimura T, Manzoor R, Muramatsu M, Yoshida R, Okamatsu M, Kida H, Takada A
    • Organizer
      XVI International Congress of Virology
    • Place of Presentation
      Montreal (Canada)
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] フィロウイルス感受性に及ぼすTIM-1多型の影響2013

    • Author(s)
      黒田誠、藤倉大輔、野依修、中山絵里、梶原将大、丸山隼輝、宮本洋子、吉田玲子、高田礼人
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場 (神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 2010年に稚内で分離された2株の高病原性鳥インフルエンザウイルスの病原性比較2013

    • Author(s)
      直亨則、梶原将大、丸山隼輝、木村享史、Manzoor Rashid、村松美笑子、岡松正敏、宮本洋子、吉田玲子、迫田義博、喜田宏、高田礼人
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場 (神戸市)
    • Related Report
      2013 Annual Research Report
  • [Book] 生体の科学2015

    • Author(s)
      梶原将大、髙田礼人
    • Publisher
      医学書院
    • Related Report
      2014 Annual Research Report
  • [Book] 実験医学2015

    • Author(s)
      梶原将大
    • Total Pages
      3
    • Publisher
      羊土社
    • Related Report
      2014 Annual Research Report
  • [Book] 実験医学 第31巻 第19号2013

    • Author(s)
      梶原将大、小川寛人、高田礼人
    • Publisher
      羊土社
    • Related Report
      2013 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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