• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of rice mutant collections using lack of DNA repair mechanisms

Research Project

Project/Area Number 25892005
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Science in genetics and breeding
Research InstitutionYamagata University

Principal Investigator

HOSHINO Tomoki  山形大学, 農学部, 准教授 (20530174)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords逆遺伝学的解析 / TILLING / 突然変異体 / DNA修復機構 / リソース / イネリソース / 逆遺伝学
Outline of Final Research Achievements

DNA double strand breaks (DSBs) by irradiation can be rejoined precisely via the classical non-homologou end-joining (cNHEJ) pathway which depends on Ku70/Ku80 and DNA ligase 4 (Lig4), while, imprecise repair often leads to the introduction of mutations including deletion, insertion and substitution at repair sites.
In this study, we examined in detail the phenotypes of ku70 and ku80 rice mutants in the early growth and heading stages. Furthermore, we observed that irradiation can lead to the effective gene modifications and small deletion at DSBs site in ku70 and ku80 rice mutants using targeting induced local lesions in genomes (TILLING). These results suggest that Ku70 and Ku80 play an important role in cNHEJ pathway in rice plants, and that the high-efficiency irradiated mutagenesis can be established using Ku70 and Ku80 rice mutants.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (6 results)

All 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] A novel <i>GmFAD3-2a</i> mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil2014

    • Author(s)
      Tomoki Hoshino, Satoshi Watanabe, Yutaka Takagi, Toyoaki Anai
    • Journal Title

      Breeding Science

      Volume: 64 Issue: 4 Pages: 371-377

    • DOI

      10.1270/jsbbs.64.371

    • NAID

      130004888870

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 高変異率を有するイネ突然変異集団の作出を目指したDNA修復欠損突然変異体の利用2014

    • Author(s)
      阿部勝磨、杉本和彦、星野友紀
    • Organizer
      東北植物学会第4回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2014-12-13 – 2014-12-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 欠失および塩基置換特異的な切断活性を有するCELIの抽出と切断活性の最適化2014

    • Author(s)
      石川衿子、阿部勝磨、杉本和彦、星野友紀
    • Organizer
      東北植物学会第4回大会
    • Place of Presentation
      山形大学
    • Year and Date
      2014-12-13 – 2014-12-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 塩基置換および欠失特異的な切断活性を有するCELIの抽出と反応条件の検討2014

    • Author(s)
      石川衿子、阿部勝磨、杉本和彦、星野友紀
    • Organizer
      第9回東北育種研究集会
    • Place of Presentation
      岩手大学
    • Year and Date
      2014-11-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA修復欠損突然変異体を利用したイネ高変異率突然変異リソース開発への試み2013

    • Author(s)
      星野友紀、杉本和彦
    • Organizer
      日本育種学会(第8回東北育種研究集会)
    • Place of Presentation
      弘前大学
    • Related Report
      2013 Annual Research Report
  • [Remarks] 山形大学農学部 星野友紀研究室 ホームページ

    • URL

      http://www.tr.yamagata-u.ac.jp/~thoshino/research.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2013-09-12   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi