• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of the mode of action of fungicides by whole genome analysis

Research Project

Project/Area Number 25892024
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Plant protection science
Research InstitutionThe University of Shiga Prefecture

Principal Investigator

IZUMITSU Kosuke  滋賀県立大学, 環境科学部, 助教 (20579263)

Research Collaborator MIYAGAWA Hisashi  京都大学, 農学研究科, 教授 (10219735)
TANAKA Chihiro  京都大学, 農学研究科, 教授 (60263133)
SUZUKI Kazumi  滋賀県立大学, 環境科学部, 教授 (90390880)
Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords殺菌剤 / 薬剤耐性 / 作用機構 / プレニル化 / 耐性化機構 / ゲラニルゲラニルトランスフェラーゼ / 全ゲノムシークエンス / 植物病原菌
Outline of Final Research Achievements

Tolnifanide is an antifungal agent with sulfonamide structure, which inhibits mycelial growth of Pleosporales fungi including Bipolaris spp. For understanding its mode of the action, we developed tolnifanide-resistant mutants of Bipolaris maydis by chemical mutagenesis and characterized them, genetically. By crossings, two mutant genes (Tfr1, Tfr2) were identified. Comparisons of whole genomes from the wild type and the resistant offsprings revealed TFR1 and TFR2 encode geranylgeranyl transferase and farnesyl diphosphate farnesyl transferase, respectively. We also revealed that polyoxin-resistant genes POL2 and POL4 encode ferrochelatase and porphobilinogen deaminase, respectively.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (13 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (10 results)

  • [Journal Article] MAPKK-encoding gene Ste7 in Bipolaris maydis is required for development and morphogenesis.2015

    • Author(s)
      Kitade Y., T. Sumita, K. Izumitsu, C. Tanaka
    • Journal Title

      Mycoscience

      Volume: 56 Issue: 2 Pages: 150-158

    • DOI

      10.1016/j.myc.2014.05.003

    • NAID

      40020402954

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of calmodulin on expressionof lignin-modifyingenzymes in Pleurotus ostreatus2014

    • Author(s)
      Suetomi, T., Sakamoto, T., Tokunaga, Y., Kameyama, T.,Honda, Y., Kamitsuji, H., Kameshita, I., Izumitsu, K., Suzuki, K., Irie, T.
    • Journal Title

      Curr. Genet.

      Volume: 14 Issue: 2 Pages: 1-14

    • DOI

      10.1007/s00294-014-0460-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The White-Rot Fungus <i>Pleurotus ostreatus</i> Transformant Overproduced Intracellular cAMP and Laccase2013

    • Author(s)
      Yao Y., T. Sakamoto, Y. Honda, Y. Kagotani, K. Izumitsu, K. Suzuki, T. Irie
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 11 Pages: 2309-2311

    • DOI

      10.1271/bbb.130470

    • NAID

      130003381926

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Tolnifanide 耐性遺伝子の解析2015

    • Author(s)
      田中千尋,吉田裕史,八木貴史,泉津弘佑,宮川恒
    • Organizer
      日本農薬学会第40回大会
    • Place of Presentation
      町田市
    • Year and Date
      2015-03-18 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 殺菌剤ポリオキシン耐性遺伝子の同定2014

    • Author(s)
      升本 宙,泉津弘佑,北出雄生,田中千尋
    • Organizer
      第14回糸状菌分子生物学コンファレンス
    • Place of Presentation
      仙台市
    • Year and Date
      2014-11-15 – 2014-11-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 全ゲノム手法を用いた殺菌剤耐性点の網羅的同定2014

    • Author(s)
      泉津弘佑,宮川恒,田中千尋
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] ウリ類炭疽病菌におけるホメオボックス転写因子CoHox1 の機能解析2014

    • Author(s)
      横山綾,泉津弘佑,谷口拓矢,多々良康香,小玉紗代,入江俊一,鈴木一実
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] トウモロコシごま葉枯病菌の2 種類の低分子量GTPase, Cdc42 およびRac1は正常な病原性発現や分生子形成に必須である2014

    • Author(s)
      北出雄生,泉津弘佑,住田卓也,湯谷智,田中千尋
    • Organizer
      平成26年度日本植物病理学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-06-02 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 全ゲノム手法を用いた殺菌剤 tolnifanide 耐性点の同定2014

    • Author(s)
      泉津弘佑,宮川恒,田中千尋
    • Organizer
      日本農薬学会第39回大会
    • Place of Presentation
      京都市
    • Related Report
      2013 Annual Research Report
  • [Presentation] ミトコンドリア局在型の Enoyl-CoA hydratase はウリ類炭疽病菌の病原性発現に必須である2013

    • Author(s)
      泉津弘佑,多々良康香,小松香織,横山 綾,谷口拓矢,丸山麻美,入江俊一,鈴木一実
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 灰色かび病菌のオートファジー関連遺伝子 BcAtg1 の機能解析2013

    • Author(s)
      住田卓也,泉津弘佑,田中千尋
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report
  • [Presentation] トウモロコシごま葉枯病菌の ChSte20 および ChCla4 遺伝子の機能解析2013

    • Author(s)
      北出雄生,泉津弘佑,住田卓也,湯谷 智,田中千尋
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report
  • [Presentation] トウモロコシごま葉枯病菌における cAMP シグナル伝達経路関連遺伝子の機能解析2013

    • Author(s)
      湯谷 智,住田卓也, 北出雄生,泉津弘佑,田中千尋
    • Organizer
      第13回糸状菌分子生物学コンファレンス
    • Place of Presentation
      つくば市
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-09-12   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi