Development of a novel treatment for infected wound based on photo-irradiation of polyphenols

• Project/Area Number: 25893019
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: SHIRATO Midori 東北大学, 大学病院, 医員 (60708501)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ポリフェノール / 過酸化水素光分解殺菌 / ヒドロキシルラジカル / プロアントシアニジン / 過酸化水素

Outline of Final Research Achievements

In the present study, an effect of proanthocyanidin (PA), one of polyphenols, on proliferation of human gingival fibroblast was investigated. It was found that PA accelerated the cell proliferation depending on the concentration. Under the harsh conditions, such as exposure to osmotic stress, starvation stress and serum deprivation stress, the short-time pretreatment with polyphenols exerted cytoprotective effect by means of suppression of yield of reactive oxygen species produced in the cells. These findings suggest that PA which exerts not only facilitatory effect of cell proliferation but also cytoprotective effect may be applied to treatment for infected wound.

Research Products

• [Journal Article] Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions. (2014)
  • Author(s): Kurauchi M, Niwano Y, Shirato M, Kanno T, Nakamura K, Egusa H, Sasaki K.
  • Journal Title: PLoS One Volume: 9 Issue: 11 Pages: e113403-e113403
  • DOI: 10.1371/journal.pone.0113403
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Kanno, T., Nakamura, K., Shirato, M., Sasaki, K., Niwano, Y
  • Journal Title: PLoS ONE Volume: 8(11) Issue: 11 Pages: e81316-e81316
  • DOI: 10.1371/journal.pone.0081316
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H2O2 (2013)
  • Author(s): HIROYO IKAI, KEISUKE NAKAMURA, TARO KANNO, MIDORI SHIRATO, LUIZ MEIRELLES, KEIICHI SASAKI, YOSHIMI NIWANO
  • Journal Title: Biocontrol Science Volume: 18 Pages: 137-141
  • NAID: 10031202904
  • Peer Reviewed
• [Presentation] プロアントシアニジンの短時間処理によるヒト繊維芽細胞に対する細胞保護作用 (2014)
  • Author(s): 倉内 美智子, 中村 圭祐, 菅野 太郎, 白土 翠, 佐々木 啓一, 庭野 吉己
  • Organizer: 新規素材探究研究会第13回セミナー
  • Place of Presentation: 横浜市
  • Year and Date: 2014-06-06
• [Presentation] Acceleration of proliferative response of 3T3-L1 mouse fibroblasts and human gingival fibroblasts by short-term exposure to proanthocyanidin (2014)
  • Author(s): Michiko Kurauchi, Keisuke Nakamura, Taro Kanno, Midori Shirato, Keiichi Sasaki,Yoshimi Niwano
  • Organizer: The 5th international symposium for interface oral science
  • Place of Presentation: Sendai,Miyagi
• [Presentation] Cytoprotective effect of short-time treatment with proanthocyanidin on human gingival fibroblasts (2014)
  • Author(s): Michiko Kurauchi, Keisuke Nakamura, Taro Kanno, Midori Shirato, Keiichi Sasaki, Yoshimi Niwano
  • Organizer: 第27回国際ポリフェノール会議・第8回タンニン会議
  • Place of Presentation: 名古屋大学（名古屋市）