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Molecular approach using multiplex PCR to diagnose intravascular infection

Research Project

Project/Area Number 25893096
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Cardiovascular medicine
Research InstitutionMie University

Principal Investigator

TANABE MASAKI  三重大学, 医学部附属病院, 准教授 (50456737)

Research Collaborator NAKAMURA Akiko  三重大学, 医学部附属病院, 臨床検査技師
Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細菌遺伝子診断 / PCR / 感染性心内膜炎 / 血管内感染症 / 培養陰性
Outline of Final Research Achievements

We evaluated utility of molecular approach using polymerase chain reaction (PCR) performed on whole blood to diagnose intravascular infection such as infective endocarditis (IE). Multiplex PCR composed of (1) broad-range PCR targeting all bacteria species, (2) bacteria specific PCR targeting 7 bacteria species, and (3) oxacillin-resistant gene were applied 10 patients with IE and 10 patients of fever of unknown origin. Bacterial genes were detected in patients with IE during phases of negative bacterial culture. Molecular approach using PCR on whole blood can be useful tool to assist diagnose and select of antibiotics in patients with culture negative vascular infection.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (3 results)

All 2015

All Presentation (3 results)

  • [Presentation] 妊娠中に発生した感染性心内膜炎の1例2015

    • Author(s)
      田辺正樹
    • Organizer
      第63回日本化学療法学会総会
    • Place of Presentation
      京王プラザホテル新宿(東京都新宿区)
    • Year and Date
      2015-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 全身性多発膿瘍を合併したPVL毒素産生MSSAによる感染性心内膜炎の1例2015

    • Author(s)
      田辺正樹
    • Organizer
      第89回日本感染症学会学術講演会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2015-04-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Panton-Valentine Leukocidin 産生黄色ブドウ球菌による感染性心内膜炎の一例2015

    • Author(s)
      志田幸太
    • Organizer
      第112回日本内科学会総会・講演会 サテライトシンポジウム
    • Place of Presentation
      みやこめっせ(京都府京都市)
    • Year and Date
      2015-04-11
    • Related Report
      2014 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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