Establishment of the mechanism analysis method of bioactive compounds based on the chemical genetics

• Project/Area Number: 25893142
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Natural medicines
• Research Institution: Hiroshima University
• Principal Investigator: YAMANO YOSHI 広島大学, 医歯薬保健学研究院(薬), 助教 (70650597)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 線虫 / C. elegans / 抗寄生虫 / 生物活性物質 / 感染症 / 活性天然物 / Magnolol / Auraptenol

Outline of Final Research Achievements

we examined antiparasitic substance from plant extracts and tried to establish a new method for elucidating the action mechanism of active compounds. In this experiment, we used C. elegans as a model of parasite and isolated 14 compounds from 3 kinds of plants. Then the action mechanism of isolated compounds examined by using deletion mutant and drug-resistant mutant strain. The result showed that 4-hydroxyphenylpyruvate dioxygenase (HPD-1) is candidate of target molecule of proctorione C.

Research Products

• [Presentation] Hepatoprotective activity of isolated compounds from Lumnitzera racemosa (2015)
  • Author(s): Ahmed DARWISH
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: デザイン・クリエイティブセンター神戸（兵庫）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] ツボクサ Centella asiatica の活性成分 (2015)
  • Author(s): 太田 涼介
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 兵庫医療大学（兵庫）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] ケナガエサカキ由来の新規ジテルペン配糖体 (2015)
  • Author(s): 阪井 史歩
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 沖縄産ヒルギカズラ葉部の光毒性抑制成分の研究 (2015)
  • Author(s): 寺道 望美
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] ツルリンドウTripterospermum japonicum地上部の成分研究 (2014)
  • Author(s): 脇本恵
  • Organizer: 日本生薬学会第61年会
  • Place of Presentation: 福岡大学薬学部（福岡）
  • Year and Date: 2014-09-13 – 2014-09-14
• [Presentation] New Flavonoid Glycosides from Linaria japonica. (2014)
  • Author(s): Retno Widyowati
  • Organizer: The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy
  • Place of Presentation: Fukuoka University（Fukuoka）
  • Year and Date: 2014-09-13