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Analyses of hematopoietic stem cell division patterns in biomimetic culture conditions and optimization of culture condition for hematopoietic stem cells

Research Project

Project/Area Number 25893234
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionResearch Institute, International Medical Center of Japan (2014)
Keio University (2013)

Principal Investigator

IKUSHIMA Yoshiko  独立行政法人国立国際医療研究センター, その他部局等, 上級研究員 (00571366)

Co-Investigator(Renkei-kenkyūsha) ARAI Fumio  九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 教授 (90365403)
HOSOKAWA Kentaro  九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 助教 (90569584)
Research Collaborator SUDA Toshio  
MACARTHUR Ben D.  
STUMPF Patrich S  
LUTOLF Matthias P.  
Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords造血幹細胞 / 娘幹細胞 / 単一細胞 / 幹細胞維持 / ニッチ分子 / 造血幹細胞分裂 / 国際情報交換 / イギリス・スイス / ニッチ
Outline of Final Research Achievements

We investigated hematopoietic stem cell (HSC) division patterns at the single cell level based on gene expression profiles of paired daughter cells (PDCs) derived from highly purified long-term HSCs. Also we analyzed the frequency of daughter stem cells with long-term bone marrow (BM) reconstitution activity in PDCs by limiting dilution competitive BM transplantation assays. We revealed that HSCs cultured on fibronectin-coated PEG hydrogel microwells with Angpt1 produce more daughter stem cells compared to HSCs cultured on fibronectin-coated normal cell culture dish without Angpt1. Our data indicated that biomimetic cell culture condition could maintain HSCs in vitro.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (3 results)

All 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Nucleostemin is indispensable for the maintenance and genetic stability of hematopoietic stem cells.2013

    • Author(s)
      Yamashita M, Nitta E, Nagamatsu G, Ikushima YM, Hosokawa K, Arai F, Suda T
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 441 Issue: 1 Pages: 196-201

    • DOI

      10.1016/j.bbrc.2013.10.032

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 単一細胞レベルでの娘細胞解析に基づくAngpt1の造血幹細胞分裂パターン決定における作用について2014

    • Author(s)
      生島芳子, Ben D MacArthur, Patrick S Stumph, 細川健太郎, Alain Roch, Matthias P Lutolf, 須田年生, 新井文用
    • Organizer
      第76回日本血液学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Single cell-based analyses of cell division pattern of hematopoietic stem cells

    • Author(s)
      Ikushima YM, MacArthur BD, Hosokawa K, Lutolf MP, Suda T, Arai F
    • Organizer
      75th Annual Meeting of the Japanese Society of Hematology
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2013 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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