Analyses of hematopoietic stem cell division patterns in biomimetic culture conditions and optimization of culture condition for hematopoietic stem cells
| Project/Area Number |
25893234
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Research Institute, International Medical Center of Japan (2014)
Keio University (2013) |
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Principal Investigator |
IKUSHIMA Yoshiko 独立行政法人国立国際医療研究センター, その他部局等, 上級研究員 (00571366)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Fumio 九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 教授 (90365403)
HOSOKAWA Kentaro 九州大学, 応用幹細胞医科学部門・幹細胞再生修復医学分野, 助教 (90569584)
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| Research Collaborator |
SUDA Toshio
MACARTHUR Ben D.
STUMPF Patrich S
LUTOLF Matthias P.
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 造血幹細胞 / 娘幹細胞 / 単一細胞 / 幹細胞維持 / ニッチ分子 / 造血幹細胞分裂 / 国際情報交換 / イギリス・スイス / ニッチ |
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| Outline of Final Research Achievements |
We investigated hematopoietic stem cell (HSC) division patterns at the single cell level based on gene expression profiles of paired daughter cells (PDCs) derived from highly purified long-term HSCs. Also we analyzed the frequency of daughter stem cells with long-term bone marrow (BM) reconstitution activity in PDCs by limiting dilution competitive BM transplantation assays. We revealed that HSCs cultured on fibronectin-coated PEG hydrogel microwells with Angpt1 produce more daughter stem cells compared to HSCs cultured on fibronectin-coated normal cell culture dish without Angpt1. Our data indicated that biomimetic cell culture condition could maintain HSCs in vitro.
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Report
(3 results)
Research Products
(3 results)
