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Analysis of sphingolipid changes in the aging of bone marrow stromal cells in a mouse hematopoietic system

Research Project

Project/Area Number 25893269
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKanazawa Medical University

Principal Investigator

OGISO Hideo  金沢医科大学, 医学部, 特定講師 (30466734)

Project Period (FY) 2013-08-30 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞膜 / 脂質ラフト / リピドミクス / マイクロドメイン / 骨髄間質細胞 / 線維芽細胞 / スフィンゴ脂質 / グリセロリン脂質 / ジアシルグリセロール / 質量分析
Outline of Final Research Achievements

We developed an approach for the comprehensive analysis of sphingolipids, glycerophospholipids and diacylglycerol molecular species in a biological sample, using LC-MS. In addition, we applied a cationic colloidal silica-bead method to analyze plasma membrane lipids from monolayer cells cultured in a 10-cm dish. The detergent-resistant fraction from the silica-bead coated membrane was analyzed to evaluate the microdomain lipids. Stromal cells from mouse bone marrow have an ability to maintain hematopoietic stem cells, but this ability decreases by aging in a process differentiating to fat cells. In this study, we analyzed some changes in lipids of membrane microdomains caused during differentiation of PA6 cells, one of the stromal cell lines, to fat cells. Result showed that ceramide level in the microdomain was elevated during the differentiation. This approach will facilitate future comprehensive analyses of membrane microdomain-associated responses in a hematopoietic system.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • Research Products

    (3 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Comparative analysis of biological sphingolipids with glycerophospholipids and diacylglycerol by LC-MS/MS2014

    • Author(s)
      Hideo Ogiso, Makoto Taniguchi, Sinichi Araya, Shinya Aoki, Lusi Oka Wardhani, Yuka Yamashita, Yoshibumi Ueda, Toshiro Okazaki
    • Journal Title

      Metabolites

      Volume: 4 Issue: 1 Pages: 98-114

    • DOI

      10.3390/metabo4010098

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] スフィンゴミエリン合成酵素欠損MEFにおける細胞膜マイクロドメイン脂質の定量的解析2014

    • Author(s)
      小木曽英夫
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] スフィンゴ脂質を中心とした膜脂質のLC-MSによる定量的解析2014

    • Author(s)
      小木曽英夫
    • Organizer
      第9回スフィンゴテラピー研究会
    • Place of Presentation
      河鹿荘ロイヤルホテル(石川県加賀市)
    • Year and Date
      2014-07-18
    • Related Report
      2014 Annual Research Report

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Published: 2013-09-12   Modified: 2019-07-29  

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