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Methods for the analysis and control of biomolecules in living cells based on molecular imaging

Research Project

Project/Area Number 26220805
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Ozawa Takeaki  東京大学, 大学院理学系研究科(理学部), 教授 (40302806)

Co-Investigator(Kenkyū-buntansha) 尾崎 倫孝  北海道大学, 保健科学研究院, 教授 (80256510)
山口 博之  北海道大学, 保健科学研究院, 教授 (40221650)
芳賀 早苗  北海道大学, 保健科学研究院, 博士研究員 (60706505)
Project Period (FY) 2014-05-30 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥195,260,000 (Direct Cost: ¥150,200,000、Indirect Cost: ¥45,060,000)
Fiscal Year 2018: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2017: ¥32,240,000 (Direct Cost: ¥24,800,000、Indirect Cost: ¥7,440,000)
Fiscal Year 2016: ¥32,240,000 (Direct Cost: ¥24,800,000、Indirect Cost: ¥7,440,000)
Fiscal Year 2015: ¥32,240,000 (Direct Cost: ¥24,800,000、Indirect Cost: ¥7,440,000)
Fiscal Year 2014: ¥69,290,000 (Direct Cost: ¥53,300,000、Indirect Cost: ¥15,990,000)
Keywordsバイオ分析 / イメージング / 光操作 / 蛍光 / 発光 / RNA / GPCR / スクリーニング / RNA / GPCR / 蛍光イメージング / アポトーシス / 生体材料 / 可視化
Outline of Final Research Achievements

In order to understand the processes of molecules in living cells as a network, we have established a new analytical method for visualizing and manipulating biomolecules in living cells. We have developed 1) a method for visualizing and quantifying a small number of biomolecules, 2) a method for controlling enzyme activity by external light, and 3) a photomanipulation method for controlling G protein-coupled receptor activity. The developed method will be a basic technology for wide fields of life science and an innovative technology in the fields of medicine, agriculture and drug discovery.

Academic Significance and Societal Importance of the Research Achievements

本研究では,個々の分子反応の素過程を追究する分子科学と,要素還元的に現象の解明を試みる生命科学との中核に位置し,分野横断的な研究領域を開拓する新規分析法を創出した.成果は国内外に情報発信を行い高い反響を得てきた.実際,国内外の異分野の研究者と共同研究が進んでおり,これまでに海外共同研究の成果として18件のoriginal papersを報告している.またプローブや光操作ツールのmaterial供与を国内外の研究者から依頼され,これまでに40件以上の研究者に遺伝子を供与してきた.さらに国内外での技術指導を積極的に展開することにより,開発した技術は分野の垣根を越えて大きな波及効果を生み出している.

Assessment Rating
Verification Result (Rating)

A+

Assessment Rating
Result (Rating)

A+: Progress in the research exceeds the initial goal. More than expected research results are expected.

Report

(10 results)
  • 2020 Research Progress Assessment (Verification Result) ( PDF )
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Abstract ( PDF )   Annual Research Report
  • Research Products

    (248 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (18 results) Journal Article (61 results) (of which Int'l Joint Research: 18 results,  Peer Reviewed: 61 results,  Open Access: 30 results,  Acknowledgement Compliant: 9 results) Presentation (163 results) (of which Int'l Joint Research: 72 results,  Invited: 53 results) Book (3 results) Remarks (3 results)

  • [Int'l Joint Research] 南洋理工大学(シンガポール)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Liverpool University(英国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Regensburg University(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 南洋理工大学(シンガポール)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Liverpool University/Manchester University(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Regensburg University(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] EPFL(スイス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Regensburg/Hannover Medical School(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Manchester University/Liverpool University(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Nanyang Technological University(シンガポール)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] EPFL(スイス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Regensburg/Hannover Medical School(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of California(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] KNAW/Leiden University/Vrije Universiteit Amsterdam(Netherlands)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] CNRS-University(France)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] Hannover Medical School/University of Regensburg(Germany)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] University of California(米国)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] EPFL(スイス)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] A Detection Method for GLUT4 Exocytosis Based on Spontaneous Split Luciferase Complementation2019

    • Author(s)
      ENDO Mizuki、MIYASAKI Masashi、LI Qiaojing、KAWAMURA Genki、OZAWA Takeaki
    • Journal Title

      Analytical Sciences

      Volume: 35 Issue: 8 Pages: 835-838

    • DOI

      10.2116/analsci.19C003

    • NAID

      130007689396

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2019-08-10
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Activation of caspase-3 during Chlamydia trachomatis-induced apoptosis at a late stage2019

    • Author(s)
      Matsuo Junji、Haga Sanae、Hashimoto Kent、Okubo Torahiko、Ozawa Takeaki、Ozaki Michitaka、Yamaguchi Hiroyuki
    • Journal Title

      Canadian Journal of Microbiology

      Volume: 65 Issue: 2 Pages: 135-143

    • DOI

      10.1139/cjm-2018-0408

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Using redox-sensitive mitochondrial cytochrome Raman bands for label-free detection of mitochondrial dysfunction.2019

    • Author(s)
      Morimoto T, Chiu LD, Kanda H, Kawagoe H, Ozawa T, Nakamura M, Nishida K, Fujita K, Fujikado T
    • Journal Title

      Analyst

      Volume: 144 Issue: 8 Pages: 25312540-25312540

    • DOI

      10.1039/c8an02213e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phenotype Profiling for Forensic Purposes: Determining Donor Sex Based on Fourier Transform Infrared Spectroscopy of Urine Traces2019

    • Author(s)
      Takamura Ayari、Halamkova Lenka、Ozawa Takeaki、Lednev Igor K.
    • Journal Title

      Analytical Chemistry

      Volume: 91 Issue: 9 Pages: 6288-6295

    • DOI

      10.1021/acs.analchem.9b01058

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Parallelized shifted‐excitation Raman difference spectroscopy for fluorescence rejection in a temporary varying system2019

    • Author(s)
      Shimada Rintaro、Nakamura Takashi、Ozawa Takeaki
    • Journal Title

      Journal of Biophotonics

      Volume: 12 Issue: 12

    • DOI

      10.1002/jbio.201960028

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] [3H]UR-DEBa176: A 2,4-Diaminopyrimidine-Type Radioligand Enabling Binding Studies at the Human, Mouse, and Rat Histamine H4 Receptors2019

    • Author(s)
      Bartole Edith、Littmann Timo、Tanaka Miho、Ozawa Takeaki、Buschauer Armin、Bernhardt G?nther
    • Journal Title

      Journal of Medicinal Chemistry

      Volume: 62 Issue: 17 Pages: 8338-8356

    • DOI

      10.1021/acs.jmedchem.9b01342

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comprehensive modeling of bloodstain aging by multivariate Raman spectral resolution with kinetics2019

    • Author(s)
      Takamura Ayari、Watanabe Daisuke、Shimada Rintaro、Ozawa Takeaki
    • Journal Title

      Communications Chemistry

      Volume: 2 Issue: 1 Pages: 115-115

    • DOI

      10.1038/s42004-019-0217-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photocleavable Cadherin Inhibits Cell-to-Cell Mechanotransduction by Light2019

    • Author(s)
      Endo Mizuki、Iwawaki Takumi、Yoshimura Hideaki、Ozawa Takeaki
    • Journal Title

      ACS Chemical Biology

      Volume: 14 Pages: 2206-2214

    • DOI

      10.1021/acschembio.9b00460

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2)Optical control of G protein-coupled receptor activities in living cells2019

    • Author(s)
      H. Yoshimura and T. Ozawa
    • Journal Title

      Progress in Photon Science

      Volume: 119 Pages: 129-138

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique Roles of β-Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers2018

    • Author(s)
      Takenouchi Osamu、Yoshimura Hideaki、Ozawa Takeaki
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-017-19130-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishing a split-luciferase-assay for PKG interaction studies2018

    • Author(s)
      A. Schramm, P.Mueller -Thuemen, T. Littmann, M. Harloff, T. Ozawa, J. Schlossmann,
    • Journal Title

      Int. J. Med. Sci.

      Volume: 19 Issue: 4 Pages: 1180-1180

    • DOI

      10.3390/ijms19041180

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Soft and Robust Identification of Body Fluid Using Fourier Transform Infrared Spectroscopy and Chemometric Strategies for Forensic Analysis2018

    • Author(s)
      Takamura Ayari、Watanabe Ken、Akutsu Tomoko、Ozawa Takeaki
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 8459-8459

    • DOI

      10.1038/s41598-018-26873-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B2018

    • Author(s)
      Natsumi Noda and Takeaki Ozawa
    • Journal Title

      Photochemistry and Photobiology

      Volume: 94 Issue: 5 Pages: 1071-1076

    • DOI

      10.1111/php.12955

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A robust split-luciferase-based cell fusion screening for discovering myogenesis-promoting molecules2018

    • Author(s)
      Li, Q., Yoshimura, H., Komiya, M., Tajiri, K., Uesugi, M., Hata, Y., Ozawa, T.
    • Journal Title

      The Analyst

      Volume: 143 Issue: 14 Pages: 3472-3480

    • DOI

      10.1039/c8an00285a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A split luciferase-based probe for quantitative proximal determination of Gαq signalling in live cells2018

    • Author(s)
      Littmann Timo、Ozawa Takeaki、Hoffmann Carsten、Buschauer Armin、Bernhardt Gunther
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 17179-17179

    • DOI

      10.1038/s41598-018-35615-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cooperative interaction among BMAL1, HSF1, and p53 protects mammalian cells from UV stress2018

    • Author(s)
      Kawamura Genki、Hattori Mitsuru、Takamatsu Ken、Tsukada Teruyo、Ninomiya Yasuharu、Benjamin Ivor、Sassone-Corsi Paolo、Ozawa Takeaki、Tamaru Teruya
    • Journal Title

      Communications Biology

      Volume: 1 Issue: 1 Pages: 204-204

    • DOI

      10.1038/s42003-018-0209-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Real-time fluorescence imaging of single-molecule endogenous non-coding RNA in living cells.2018

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Journal Title

      Methods Mol. Biol.

      Volume: 1649 Pages: 337-347

    • DOI

      10.1007/978-1-4939-7213-5_22

    • ISBN
      9781493972128, 9781493972135
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nano-Materials for Bioimaging2018

    • Author(s)
      OZAWA Takeaki
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 2 Pages: 125-126

    • DOI

      10.2116/analsci.34.125

    • NAID

      130006341949

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of necroptosis in ligand-mediated and hypoxia-induced injury of hepatocytes using a novel optic probe detecting receptor-interacting protein (RIP)1/RIP3 binding2018

    • Author(s)
      S. Haga, A. Kanno, T. Ozawa, N. Morita, M. Asano, M. Ozaki
    • Journal Title

      Oncology Research

      Volume: 26 Issue: 3 Pages: 503-513

    • DOI

      10.3727/096504017x15005102445191

    • NAID

      120006473611

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photo-Activatable Akt Probe: A New Tool to Study the Akt-Dependent Physiopathology of Cancer Cells2018

    • Author(s)
      Haga Sanae、Ozawa Takeaki、Morita Naoki、Asano Mami、Jin Shigeki、Yimin、Ozaki Michitaka
    • Journal Title

      Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics

      Volume: 26 Issue: 3 Pages: 467-472

    • DOI

      10.3727/096504017x15040166233313

    • NAID

      120006473610

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid in vivo lipid/carbohydrate quantification of single microalgal cell by Raman spectral imaging to reveal salinity-induced starch-to-lipid shift2017

    • Author(s)
      Liang-da Chiu, Shih-Hsin Ho, Rintaro Shimada, Nan-Qi Ren, Takeaki Ozawa
    • Journal Title

      Biotechnology for Biofuels

      Volume: 10 Issue: 1 Pages: 9-9

    • DOI

      10.1186/s13068-016-0691-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae2017

    • Author(s)
      Y. Fukutani, J. Ishii, A. Kondo, T. Ozawa, H. Matsunami, M. Yohda
    • Journal Title

      Biotechnol. Bioeng.

      Volume: 114 Issue: 6 Pages: 1354-1361

    • DOI

      10.1002/bit.26255

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Single-shot phase-shifting incoherent digital holography2017

    • Author(s)
      Tatsuki Tahara, Takeya Kanno, Yasuhiko Arai and Takeaki Ozawa
    • Journal Title

      Journal of Optics

      Volume: 19 Issue: 6 Pages: 065705-065705

    • DOI

      10.1088/2040-8986/aa6e82

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optogenetic interrogation reveals separable G-protein-dependent and -independent signalling linking G-protein-coupled receptors to the circadian oscillator2017

    • Author(s)
      Bailes Helena J.、Milosavljevic Nina、Zhuang Ling-Yu、Gerrard Elliot J.、Nishiguchi Tomoki、Ozawa Takeaki、Lucas Robert J.
    • Journal Title

      BMC Biology

      Volume: 15 Issue: 1 Pages: 40-40

    • DOI

      10.1186/s12915-017-0380-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In Search of NPY Y4R Antagonists: Incorporation of Carbamoylated Arginine, Aza-Amino Acids, or d-Amino Acids into Oligopeptides Derived from the C-Termini of the Endogenous Agonists2017

    • Author(s)
      Kuhn Kilian K.、Littmann Timo、Dukorn Stefanie、Tanaka Miho、Keller Max、Ozawa Takeaki、Bernhardt G?nther、Buschauer Armin
    • Journal Title

      ACS Omega

      Volume: 2 Issue: 7 Pages: 3616-3631

    • DOI

      10.1021/acsomega.7b00451

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spectral Mining for Discriminating Blood Origins in the Presence of Substrate Interference via Attenuated Total Reflection Fourier Transform Infrared Spectroscopy: Postmortem or Antemortem Blood?2017

    • Author(s)
      Takamura Ayari、Watanabe Ken、Akutsu Tomoko、Ikegaya Hiroshi、Ozawa Takeaki
    • Journal Title

      Analytical Chemistry

      Volume: 89 Issue: 18 Pages: 9797-9804

    • DOI

      10.1021/acs.analchem.7b01756

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic Monitoring of p53 Translocation to Mitochondria for the Analysis of Specific Inhibitors Using Luciferase-fragment Complementation.2017

    • Author(s)
      Natsumi Noda, Raheela Awais, Robert Sutton, Muhammad Awais and Takeaki Ozawa
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 114 Issue: 12 Pages: 2818-2827

    • DOI

      10.1002/bit.26407

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A genetic screen to discover SUMOylated proteins in living mammalian cells2017

    • Author(s)
      Komiya M, Ito A, Endo M, Hiruma D, Hattori M, Saitoh H, Yoshida M, Ozawa T.
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 17443-17443

    • DOI

      10.1038/s41598-017-17450-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Strategies for development of optogenetic systems and their applications2017

    • Author(s)
      Endo M.、Ozawa T.
    • Journal Title

      Journal of Photochemistry and Photobiology C: Photochemistry Reviews

      Volume: 30 Pages: 10-23

    • DOI

      10.1016/j.jphotochemrev.2016.10.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Luminescent sensors for single cell analysis.2017

    • Author(s)
      Ozawa T.
    • Journal Title

      Progress Photon Sci

      Volume: 115 Pages: 97-108

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 光誘導性二量体・多量体形成システムを用いた受容体タンパク質活性の制御2017

    • Author(s)
      遠藤瑞己,小澤岳昌
    • Journal Title

      生体の科学

      Volume: 68 Pages: 97-108

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein expression guided chemical profiling of living cells by hybrid fluorescence-Raman microscopy2017

    • Author(s)
      Liang-da Chiu , Taro Ichimura , Takumasa Sekiya , Hiroaki Machiyama , Tomonobu Watanabe , Hideaki Fujita , Takeaki Ozawa, Katsumasa Fujita
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 43569-43569

    • DOI

      10.1038/srep43569

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Situ Characterization of Bak Clusters Responsible for Cell Death Using Single Molecule Localization Microscopy2016

    • Author(s)
      Y. Nasu, A. Benke, S. Arakawa, G. Yoshida, G. Kawamura, S. Manley, S. Shimizu, T. Ozawa
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 27505-27505

    • DOI

      10.1038/srep27505

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres.2016

    • Author(s)
      Yamada, T., Yoshimura, H., Shimada, R., Hattori, M., Eguchi, T., Fujiwara, K., Kusumi, A. and Ozawa, T.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 38910-38910

    • DOI

      10.1038/srep38910

    • NAID

      120005997833

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Confocal Bioluminescence Imaging for Living Tissues with a Caged Substrate of Luciferin.2016

    • Author(s)
      Hattori, M., Kawamura, G., Kojima, R., Kamiya, M., Urano, Y. and Ozawa, T.
    • Journal Title

      Anal. Chem.

      Volume: 88(12) Issue: 12 Pages: 6231-6238

    • DOI

      10.1021/acs.analchem.5b04142

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Label-free versus conventional cellular assays: functional investigations on the human histamine H1 receptor2016

    • Author(s)
      S. Lieb, T. Littmann, N. Plank, J. Felixberger, M. Tanaka, T. Schafer, S. Krief, S. Elz, K. Friedland, G. Bernhardt, J. Wegener, T. Ozawa and A. Buschauer,
    • Journal Title

      Pharmacol. Res.

      Volume: 114 Pages: 13-26

    • DOI

      10.1016/j.phrs.2016.10.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Bioluminescent Indicator for Highly Sensitive Analysis of Estrogenic Activity in a Cell-based Format.2016

    • Author(s)
      Takenochi, O., Kanno, A., Takakura, H., Hattori, M. and Ozawa, T.
    • Journal Title

      Bioconju.Chem.

      Volume: 7 Issue: 11 Pages: 2689-2694

    • DOI

      10.1021/acs.bioconjchem.6b00466

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Attenuation of Chemokine Receptor Function and Surface Expression as an Immunomodulatory Strategy Employed by HCMV is Linked to vGPCR US282016

    • Author(s)
      T. Frank, A. Reichel, O. Larsen, A.-C. Stilp, M. M. Rosenkilde, T. Stamminger, T. Ozawa and N. Tschammer,
    • Journal Title

      Cell Commun. Signal

      Volume: 14 Issue: 1 Pages: 31-31

    • DOI

      10.1186/s12964-016-0154-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of RNA dynamics in living cells using PUM-HD and fluorescent protein reconstitution technique.2016

    • Author(s)
      H. Yoshimura and T. Ozawa
    • Journal Title

      Methods Enzymol.

      Volume: 572 Pages: 65-85

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-Coupled Receptor for High-Throughput Screening and Analysis2016

    • Author(s)
      H. Mitsuru、T. Ozawa
    • Journal Title

      Methods Mol. Biol.

      Volume: 1461 Pages: 195-202

    • DOI

      10.1007/978-1-4939-3813-1_16

    • ISBN
      9781493938117, 9781493938131
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genetically encoded bioluminescent indicator for illuminating proinflammatory cytokines2016

    • Author(s)
      S. B. Kim, T. Ozawa and Y. Umezawa
    • Journal Title

      MethodsX

      Volume: 3 Pages: 483-489

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation.2016

    • Author(s)
      Yusuke Nasu, Yoichi Asaoka, Misako Namae, Hiroshi Nishina, Hideaki Yoshimura and Takeaki Ozawa
    • Journal Title

      Anal Chem

      Volume: 88 Issue: 1 Pages: 838-844

    • DOI

      10.1021/acs.analchem.5b03367

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An optogenetic system for interrogating the temporal dynamics of Akt.2015

    • Author(s)
      Y. Katsura, H. Kubota, K. Kunida, A. Kanno, S. Kuroda, T. Ozawa
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 14589-14589

    • DOI

      10.1038/srep14589

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CRY drives cyclic CK2-mediated BMAL1 phosphorylation to control the mammalian circadian clock2015

    • Author(s)
      1)Tamaru T, Hattori M, Honda K, Nakahata Y, Sassone-Corsi P, van der Horst GTJ, Ozawa T, Takamatsu K
    • Journal Title

      PLoS Biology

      Volume: 13 Issue: 11 Pages: e1002293-e1002293

    • DOI

      10.1371/journal.pbio.1002293

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Recruitment of β-Arrestin 1 and 2 to the β2-Adrenoceptor: Analysis of 65 Ligands.2015

    • Author(s)
      T. Littmann, M. Gottle, M.T. Reinartz, S. Kalble, I. W. Wainer, T. Ozawa, R. Seifert.
    • Journal Title

      J Pharmacol Exp Ther.

      Volume: 355 Issue: 2 Pages: 183-90

    • DOI

      10.1124/jpet.115.227959

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans2015

    • Author(s)
      Tomoki Nishiguchi, Toshimichi Yamada, Yusuke Nasu, Mashiho Ito, Hideaki Yoshimura, Takeaki Ozawa
    • Journal Title

      Journal of Biomedical Optics

      Volume: 20 Issue: 10 Pages: 101205-101205

    • DOI

      10.1117/1.jbo.20.10.101205

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells2015

    • Author(s)
      Kodaka, M.; Yang, Z.; Nakagawa, K.; Maruyama, J.; Xu, X.; Sarkar, A.; Ichimura, A.; Nasu, Y.; Ozawa, T.; Iwasa, H.; Ishigami-Yuasa, M.; Ito, S.; Kagechika, H.; Hata, Y.
    • Journal Title

      Exp. Cell. Res.

      Volume: 336 Issue: 2 Pages: 171-181

    • DOI

      10.1016/j.yexcr.2015.06.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source2015

    • Author(s)
      Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc, Takeaki Ozawa and Hideaki Kano
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 4 Pages: 299-305

    • DOI

      10.2116/analsci.31.299

    • NAID

      130005061528

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-throughput Live Cell Imaging and Analysis for Temporal Reaction of G Protein-coupled Receptor Based on Split Luciferase Fragment Complementation2015

    • Author(s)
      Hattori, M. and Ozawa, T.
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 4 Pages: 327-330

    • DOI

      10.2116/analsci.31.327

    • NAID

      130005061535

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] “Molecular Imaging for Bioanalysis”2015

    • Author(s)
      T. ozawa
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 4 Pages: 243-243

    • DOI

      10.2116/analsci.31.243

    • NAID

      130005061523

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell-Based Assays and Animal Models for GPCR Drug Screening2015

    • Author(s)
      H. Takakura, M. Hattori, M. Tanaka and T. Ozawa
    • Journal Title

      Methods Mol. Biol.

      Volume: 1272 Pages: 257-270

    • DOI

      10.1007/978-1-4939-2336-6_18

    • ISBN
      9781493923359, 9781493923366
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioluminescent tools for the analysis of G-protein-coupled receptor and arrestin interactions2015

    • Author(s)
      Hattori, M. and Ozawa, T.
    • Journal Title

      RSC Adv.

      Volume: 5 Issue: 17 Pages: 12655-12663

    • DOI

      10.1039/c4ra14979c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous time-lamination imaging of protein association using a split fluorescent timer protein2015

    • Author(s)
      A. Takamura, M. Hattori, H. Yoshimura and T. Ozawa
    • Journal Title

      Anal. Chem.

      Volume: 87 Issue: 6 Pages: 3366-3372

    • DOI

      10.1021/ac504583t

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Multimodal and multiplex spectral imaging of rat cornea ex vivo using a white-light laser source2015

    • Author(s)
      Hiroki Segawa, Yuichi Kaji, Philippe Leproux, Vincent Couderc, Takeaki Ozawa, Tetsuro Oshika and Hideaki Kano
    • Journal Title

      Journal of Biophotonics

      Volume: 8 Issue: 9 Pages: 1-4

    • DOI

      10.1002/jbio.201400059

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure-bias relationships for fenoterol stereoisomers in six molecular and cellular assays at the β2-adrenoceptor.2015

    • Author(s)
      M.T. Reinartz, S. Kalble, T. Littmann, T. Ozawa, S. Dove, V. Kaever, I.W. Wainer, R. Seifert
    • Journal Title

      Naunyn Schmiedebergs Arch Pharmacol

      Volume: 388 Issue: 1 Pages: 51-65

    • DOI

      10.1007/s00210-014-1054-5

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] p62/SQSTM1 Plays a Protective Role in Oxidative Injury of Steatotic Liver in a Mouse Hepatectomy Model.2014

    • Author(s)
      Haga S, Ozawa T, Yamada Y, Morita N, Nagashima I, Inoue H, Inaba Y, Noda N, Abe R, Umezawa K, Ozaki M.
    • Journal Title

      Antioxid Redox Signal.

      Volume: 21 Issue: 18 Pages: 2515-2530

    • DOI

      10.1089/ars.2013.5391

    • NAID

      120005677747

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developing a potentially immunologically-inert tetracycline-regulatable viral vector for gene therapy in the peripheral nerve2014

    • Author(s)
      S. Hoyng, S. Gnavi, F. Winter, R. Eggers, T. Ozawa, A. Zaldumbide, R. Hoeben, M. Malessy, and J. Verhaagen
    • Journal Title

      Gene Therapy

      Volume: 21 Issue: 6 Pages: 549-557

    • DOI

      10.1038/gt.2014.22

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli2014

    • Author(s)
      Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
    • Journal Title

      Molecular cell

      Volume: 53 Issue: 3 Pages: 393-406

    • DOI

      10.1016/j.molcel.2014.01.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assay methods for small ubiquitin-like modifier (SUMO)-SUMO-interacting motif (SIM) interactions in vivo and in vitro using a split-luciferase complementation system2014

    • Author(s)
      Hirohama M, Voet AR, Ozawa T, Saitoh H, Nakao Y, Zhang KY, Ito A, Yoshida M.
    • Journal Title

      Anal Biochem.

      Volume: 448 Pages: 92-4

    • DOI

      10.1016/j.ab.2013.12.009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Split Luciferase Complementation for Analysis of Intracellular Signaling2014

    • Author(s)
      M. Hattori and T. Ozawa
    • Journal Title

      Anal. Sci

      Volume: 30 Pages: 539-543

    • NAID

      130004442285

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Methods of split-reporter reconstitution for the analysis of biomolecules.2014

    • Author(s)
      H. Yoshimura and T. Ozawa
    • Journal Title

      Chem. Record

      Volume: 14 Issue: 3 Pages: 492-501

    • DOI

      10.1002/tcr.201402001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Luminescent Sensors and Optical Switches for Single Cell Analysis.2019

    • Author(s)
      T. Ozawa
    • Organizer
      Seminar at Kansas State University (KSU seminar)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 生命の神秘を光で探る―オプトバイオアナリシス―2019

    • Author(s)
      T. Ozawa
    • Organizer
      東京大学理学部公開講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Drug efficacy estimation based on dynamic ligand-receptor-G protein interactions2019

    • Author(s)
      Tomoki Nishiguchi, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dynamic ligand-receptor-effector protein interactions detected with single molecule fluorescence microscopy2019

    • Author(s)
      Tomoki Nishiguchi, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A bioluminescence method for sensing GLUT4 on plasma membrane using spontaneous split luciferase complementation2019

    • Author(s)
      Masashi Miyasaki, Genki Kawamura, Mizuki Endo, Takeaki Ozawa
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Novel optical techniques to explore biological function in single cells2019

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      The 4th STEPS Symposium on Photon Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein-based luminescent sensors for single cell analysis.2018

    • Author(s)
      T. Ozawa
    • Organizer
      JSPS-NTS Bilateral Symposium on Functional Materials Chemistry
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光で生体分子を操作し観察する新たな細胞解析技術2018

    • Author(s)
      小澤 岳昌
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Opto-bioanalysis: Imaging and controlling GPCR activities in living cells.2018

    • Author(s)
      T. Ozawa
    • Organizer
      IUBMB
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生細胞内GPCR動態の可視化と制御2018

    • Author(s)
      小澤 岳昌
    • Organizer
      生理研研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 光でタンパク質を操作し観察する細胞解析技術―オプトバイオアナリシス―2018

    • Author(s)
      小澤 岳昌
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Imaging and Controlling Protein Activities in Living Cells: Opto-bioanalysis2018

    • Author(s)
      T. Ozawa
    • Organizer
      6th UT-UDS Joint Symposium on “Symmetry and Asymmetry in Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and Manipulating Intracellular signals in Single Live Cells with External Light.2018

    • Author(s)
      T. Ozawa
    • Organizer
      the 10th International Forum on Post-Genomic Technology (IFPT’10) and the 11th International Workshop on Approaches to Single-Cell Analysis (IWSC’11)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Design of Luminescent Sensors and Optical Switches for Single Cell Analysis.2018

    • Author(s)
      T. Ozawa
    • Organizer
      4th International Symposium on Molecular Imaging and Nanomedicine
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Optical Techniques to Explore the Functions of G Protein-coupled Receptors (GPCRs).2018

    • Author(s)
      T. Ozawa
    • Organizer
      5th International Conference on Innovative Biology Medicine and Engineering (ICIBME)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel design of luminescent sensors and optical switches for cellular analysis-Opto-bioanalysis-2018

    • Author(s)
      T. Ozawa
    • Organizer
      Hwasun Optical & Molecular Imaging Workshop and Symposium (HOWS)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and Manipulating GPCRs in Live Cells with External Light.2018

    • Author(s)
      T. Ozawa
    • Organizer
      10th Singapore International Chemistry Conference (SICC10)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single molecule detection of transient formation and dissociation of GPCR-G protein complex2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      14th Asian Conference on Analytical Sciences (ASIANALYSIS XIV)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 近赤外ラマン分光と反応速度論を考慮した多変量スペクトル分解による血液試料の法科学的陳旧度推定2018

    • Author(s)
      髙村彩里、渡邉大助、島田林太郎、窪田聡、阿久津智子、小澤岳昌
    • Organizer
      平成30年度日本分光学会年次講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生細胞内におけるGPCR活性化状態と多量体形成状態の同時評価法の開発2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      第78回分析化学討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A TECHNOLOGY FOR RNA LABELING IN LIVING CELLS TO MONITOR SINGLE-MOLECULE DYNAMICS UNDER FLUORESCENCE MICROSCOPY2018

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      Focus on Microscopy (FOM) 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid confocal Raman Hyperspectral Imaging by a Mechanical Streak Camera2018

    • Author(s)
      Rintaro Shimada, Liang-da Chiu, and Takeaki Ozawa
    • Organizer
      ICORS(International Conference On Raman Spectroscopy) 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a molecular tool to manipulate membrane proteins trafficking in live cells2018

    • Author(s)
      Yang Li, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      Fifth Asian Chemical Biology Conference (ACBC-5)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of GPCRs by live-cell single-molecule imaging2018

    • Author(s)
      Hiroaki Toyota, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      RSC Tokyo International Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a molecular tool to manipulate membrane proteins trafficking in live cells2018

    • Author(s)
      Yang Li, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      RSC Tokyo International Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A detection method of GLUT4 exocytosis based on spontaneous split luciferase complementation2018

    • Author(s)
      Masashi Miyasaki, Genki Kawamura, Takeaki Ozawa
    • Organizer
      RSC Tokyo International Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Assay system for individual signaling activity of G-protein coupled receptor using single molecule imaging2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分割型ルシフェラーゼの自発的再構成を用いた糖輸送タンパク質GLUT4の細胞膜移行検出法の開発2018

    • Author(s)
      宮崎将司、河村玄気、小澤岳昌
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生細胞内RNA可視化プローブを用いた機能性RNAの1分子追跡2018

    • Author(s)
      吉村英哲、山田俊理、島田林太郎、小澤岳昌
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞内1分子計測によるGタンパク質共役型受容体の機能解析2018

    • Author(s)
      豊田宏明、吉村英哲、小澤岳昌
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 機能解析を志向した生細胞内RNAの1分子標識・動態分析法2018

    • Author(s)
      吉村英哲、山田俊理、江口正敏、島田林太郎、小澤岳昌
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gタンパク質共役型受容体の薬剤依存的な活性化状態変化の一分子測定2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メカニカルストリークカメラを用いた顕微ラマン分光系の開発と生細胞への応用2018

    • Author(s)
      島田 林太郎,邱 亮達, 小澤 岳昌
    • Organizer
      分光学会生細胞分光部会 合同研究会「Medical Optics & Spectroscopy 2018」
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of real-time motion of signal transduction molecule Akt in living cells to reveal its functioning mechanism2018

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A cell fusion assay based on split luciferase complementation for screening myogenesis-promoting compounds2018

    • Author(s)
      Qiaojing Li, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      The Ninth International Forum on Chemistry of Functional Organic Chemicals (IFOC-9)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Optogenetics approach for multi-omics analysis of Akt kinase regulated metabolic pathways2018

    • Author(s)
      Genki Kawamura, Takeaki Ozawa
    • Organizer
      The Ninth International Forum on Chemistry of Functional Organic Chemicals (IFOC-9)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Live-cell single molecule detection of ligand-induced protein-protein interactions using fluorescence microscopy2018

    • Author(s)
      Tomoki Nishiguchi, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      The Ninth International Forum on Chemistry of Functional Organic Chemicals (IFOC-9)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Kinetic Multivariate Deconvolution of Raman Spectra for Forensic Modeling of Blood Aging2018

    • Author(s)
      Ayari Takamura, Daisuke Watanabe, Rintaro Shimada, Takeaki Ozawa
    • Organizer
      The Ninth International Forum on Chemistry of Functional Organic Chemicals (IFOC-9)
    • Related Report
      2018 Annual Research Report
  • [Presentation] CREB活性化を検出する生物発光プローブを用いた1細胞イメージング2018

    • Author(s)
      野田なつみ、小澤岳昌
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Circadian clock dependent activation of stress responsive pathways protects cell from ultraviolet irradiation2018

    • Author(s)
      河村玄気、田丸輝也、高松研、小澤岳昌
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A single molecule imaging approach to understand signal transduction on the plasma membrane in living cells2018

    • Author(s)
      Hideaki Yoshimura
    • Organizer
      International Symposium on Nano Medicine 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分子動態解析による細胞内分子作動機構の解明-生細胞1分子イメージングによるアプローチ-2018

    • Author(s)
      吉村英哲
    • Organizer
      第95回創薬科学セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Development of the methods for imaging and optical control of biomolecules based on protein chemistry2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      The 98th CSJ Annual Meeting
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Opto-bioanalysis: Imaging and controlling protein activities in living cells.2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      3rd STEPS Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Monitoring GPCR-β-arrestin interactions and cell fusion events for chemical library screening by split luciferase reconstitution.2018

    • Author(s)
      Takeaki Ozawa
    • Organizer
      PITTCON2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 1分子解析によりGPCRシグナル伝達の多様性を解明する.2018

    • Author(s)
      豊田宏明, 吉村英哲, 小澤岳昌
    • Organizer
      第20回生命化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 内在性EGFRの光による活性制御を可能とする光二量体化抗体の開発2018

    • Author(s)
      太田椿・遠藤瑞己・吉村英哲・小澤岳昌
    • Organizer
      第20回生命化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分割型ルシフェラーゼの自発的再構成を用いたGLUT4の細胞膜存在量発光測定法の開発2018

    • Author(s)
      宮崎将司・河村玄気・小澤岳昌
    • Organizer
      第20回生命化学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Simultaneous detection of the GPCR stoichiometry and its activity with single molecule imaging2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      Keystone symosia Conference (B8: GPCR Structure and Function: Taking GPCR Drug Development and Discovery to the Next Level)
    • Related Report
      2017 Annual Research Report
  • [Presentation] A TECHNOLOGY FOR RNA LABELING IN LIVING CELLS TO MONITOR SINGLE-MOLECULE DYNAMICS UNDER FLUORESCENCE MICROSCOPY2018

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      Focus on Microscopy 2018
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dual color single molecule imaging to track the GPCR-G protein interactions2018

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      Focus on Microscopy 2018
    • Related Report
      2017 Annual Research Report
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      Asia and Oceania Conference of Photobiology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光で操作し観察する新たな細胞解析技術―OPTO-BIOANALYSIS―2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      第2回Molecular Devices Seminar
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Protein-based luminescent sensors for library screening2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      Asian-CHIP 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors and optical switches for intracellular signaling analysis.2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      CBC Seminar
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analytical Probes for In Vivo Optical Functional Imaging.2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      第2回発光ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生体分子の時空間制御2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      イメージングブートキャンプ
    • Related Report
      2017 Annual Research Report
  • [Presentation] Opto-bioanalysis of receptor activation and signaling using genetically-encoded probes.2017

    • Author(s)
      Takeaki Ozawa
    • Organizer
      Chemical Biology Seminar
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Telomeric-repeat containing RNA captures hnRNPA1 around telomeres in living cells: a single molecule imaging study.2017

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Rintaro Simada, Takeaki Ozawa
    • Organizer
      内藤コンファレンス
    • Related Report
      2017 Annual Research Report
  • [Presentation] Hybrid Fluorescence-Raman Imaging to Correlate Biochemical Information to Protein Expression2017

    • Author(s)
      Liang-da Chiu, Taro Ichimura, Hideaki Fujita, Takeaki Ozawa, Katsumasa Fujita
    • Organizer
      The 24th Congress of the International Comission for Optics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a bioluminescent probe to monitor p53 translocation into mitochondria using luciferase-fragment complementation assays.2017

    • Author(s)
      野田なつみ・Raheela Awais・ Robert Sutton・Muhammad Awais・小澤岳昌
    • Organizer
      第32回生体機能関連化学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Application of reconstitution of split fluorescent protein fragments to identify mammalian SUMOylated proteins.2017

    • Author(s)
      小宮麻希・比留間大祐・服部満・小澤岳昌
    • Organizer
      RSC Tokyo International Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A cell fusion evaluation method based on split luciferase reconstitution and its application to screening of myogenesis promoting compounds2017

    • Author(s)
      Qiaojing Li・Hideaki Yoshimura・Takeaki Ozawa
    • Organizer
      RSC Tokyo International Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of the single molecule imaging to evaluate signal transduction efficacy of GPCR in living cells2017

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      RSC Tokyo International Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA結合タンパク質PUM-HDを用いた生細胞内RNA可視化分析法2017

    • Author(s)
      吉村 英哲、山田 俊理、児島 友哉、島田 林太郎、小澤 岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 多スペクトル並列検出を用いた単焦点共焦点ラマン顕微分光計によるハイパースペクトルイメージング高速化の試み2017

    • Author(s)
      島田 林太郎・邱 亮達・小澤 岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 二分割蛍光タンパク質の再構成に基づいた遺伝的解析によるSUMO化タンパク質の探索.2017

    • Author(s)
      小宮麻希・比留間大祐・服部満・小澤岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] An assay method for evaluating cell fusion events using protein trans splicing-based split luciferase reconstitution2017

    • Author(s)
      Qiaojing Li・Hideaki Yoshimura・Takeaki Ozawa
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] βアレスチンを介した膜受容体の細胞内輸送を解析する光学的分析法の開発2017

    • Author(s)
      竹之内 修, 吉村 英哲, 小澤 岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 一分子観察に基づく細胞内におけるGタンパク質共役型受容体の活性化状態の測定2017

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Single-cell bioluminescent imaging analysis of circadian response to UV irradiation for elucidation of the synchronization mechanism.2017

    • Author(s)
      河村玄気・田丸輝也・小澤岳昌
    • Organizer
      日本分析化学会第66年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dynamics and localization of a non-coding RNA TERRA in living cells revealed by single molecule imaging2017

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Rintaro Simada, Takeaki Ozawa
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evaluation of G-protein coupled receptor (GPCR) signaling activity based on dual color single molecule imaging of GPCR and G-protein2017

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Ultra-violet irradiation triggers circadian clock dependent activation of stress responsive transcription factors to express adaptive responses.2017

    • Author(s)
      河村玄気・田丸輝也・服部満・高松研・小澤岳昌
    • Organizer
      第24回日本時間生物学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Parallelised Raman detection methods for biomedical studies2017

    • Author(s)
      Liang-da Chiu, Takeaki Ozawa
    • Organizer
      第15回医用分光額研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Sustained monitoring of p53 translocation into mitochondria using luciferase-fragment complementation.2017

    • Author(s)
      野田なつみ・Raheela Awais・ Robert Sutton・Muhammad Awais・小澤岳昌
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リガンド刺激によるGPCR-Gタンパク質複合体形成の一分子解析2017

    • Author(s)
      西口知輝、吉村英哲、小澤岳昌
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Circadian time dependent activation of stress responsive transcription factors regulates adaptive response to ultra-violet irradiation2017

    • Author(s)
      河村玄気・田丸輝也・服部満・高松研・小澤岳昌
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Luminescent Sensors and Imaging Technologies for Drug Discovery.2017

    • Author(s)
      小澤岳昌
    • Organizer
      PITTCON2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Opto-bioanalysis of receptor activation and signaling using genetically-encoded probes.2017

    • Author(s)
      小澤岳昌
    • Organizer
      Innovation Mixers (London, Imperial College London)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein-based luminescent sensors for single cell analysis2017

    • Author(s)
      小澤岳昌
    • Organizer
      NTU-SNU-UTokyo Chemistry Symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of repebody for clathrin coated pit2016

    • Author(s)
      江藤和馬・小澤岳昌・吉村英哲・福田一貴
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of a Bioluminescent Indicator for Highly Sensitive and Rapid Detection of Estrogenic Activity in Living Cells2016

    • Author(s)
      竹之内修・菅野憲・高倉栄男・服部満・小澤岳昌
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of red-shifted mutant luciferases derived from Pyrearinus termitilluminans2016

    • Author(s)
      西口知輝・山田俊理・伊藤真志保・那須雄介・吉村英哲・小澤岳昌
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Development of bioluminescent probes to analyze integral role of heat-shock factor 1 in the synchronization of circadian clock.2016

    • Author(s)
      河村玄気・服部満・田丸輝也・小澤岳昌
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] The search for novel SUMOylated proteins based on reconstitution of split fluorescence proteins2016

    • Author(s)
      小宮麻希・比留間大祐・服部満・小澤岳昌
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生物発光イメージングシステムを利用した生細胞ハイスループット解析法の開発2016

    • Author(s)
      服部満・小澤岳昌
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of single molecule dynamic to reveal the functional mechanism of telomeric repeat-containing RNA2016

    • Author(s)
      YOSHIMURA, Hideaki; YAMADA, Toshimichi; OZAWA, Takeaki
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Hybrid Fluorescence-Raman Microscopy for the Visualisation of Protein-Metabolome Interaction2016

    • Author(s)
      Liang-da Chiu, Taro Ichimura, Hideaki Fujita, Takeaki Ozawa, Katsumasa Fujita
    • Organizer
      Focus on Microscopy 2016
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SINGLE-MOLECULE LIVE-CELL IMAGING OF A NON-CODING RNA2016

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Rintaro Shimada, Takeaki Ozawa
    • Organizer
      Focus on Microscopy 2016
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Protein-based luminescent sensors for single cell analysis2016

    • Author(s)
      小澤岳昌
    • Organizer
      Asianalysis XIII Chiang-Mai (Thailand)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Opto-bioanalysis of receptor activation and signaling using genetically-encoded probes2016

    • Author(s)
      小澤岳昌
    • Organizer
      Japan-Taiwan Medical Spectroscopy International Symposium (JTMSIS)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2016

    • Author(s)
      小澤岳昌
    • Organizer
      ETHZ-UTokyo Joint Symposium of Frontier Chemistry
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cry2を用いた細胞内シグナルの光制御法2016

    • Author(s)
      小澤岳昌
    • Organizer
      第8回光操作研究会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Split luciferase detection of receptor activation and signaling2016

    • Author(s)
      小澤岳昌
    • Organizer
      Bayliss Starling Symposium, The Physiological Society (UK)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2016

    • Author(s)
      小澤岳昌
    • Organizer
      Third Biennial FB3 Conference, Tianjin University
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生体分子の時空間制御とライブイメージング2016

    • Author(s)
      小澤岳昌
    • Organizer
      イメージングブートキャンプ
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 生体分子の可視化と光操作法から展望する未来の光分析技術2016

    • Author(s)
      小澤岳昌
    • Organizer
      第76回分析化学討論会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 生体分子を観る・操作する光分析科学2016

    • Author(s)
      小澤岳昌
    • Organizer
      分子研シンポジウム
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Development of a protein module for controlling the spatiotemporal activation of TNFR1 with external light2016

    • Author(s)
      児玉祐来,遠藤瑞己,吉村英哲,小澤岳昌
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Quantitative single molecular analysis to evaluate the signal transduction efficiencies of a G protein coupled receptor2016

    • Author(s)
      西口知輝,吉村英哲,小澤岳昌
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of a bioluminescence-driven cellular signaling manipulation method using an optogenetic tool.2016

    • Author(s)
      河村玄気,桂嘉宏,小澤岳昌
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Optical manipulation of neurite outgrowth in vivo using light-dependent protein oligomerizaion2016

    • Author(s)
      遠藤瑞己,小澤岳昌
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Hybrid fluorescence-Raman imaging to correlate biochemical information to protein expression2016

    • Author(s)
      邱亮達,市村垂生、藤田英明、小澤岳昌、藤田克昌
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Analysis and manipulation of cellular signalling via a luciferase-driven optogenetics.2016

    • Author(s)
      河村玄気,桂嘉宏,小澤岳昌
    • Organizer
      ASIANALYSIS XIII
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis on time-dependent molecular responses during mammalian circadian clock synchronization by ultra-violet irradiation.2016

    • Author(s)
      河村玄気,田丸輝也,服部満,小澤岳昌
    • Organizer
      第39回日本分子生物学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Fluorescent protein guided chemical analysis of living cells by hybrid fluorescence-Raman microscopy2016

    • Author(s)
      邱亮達,市村垂生、藤田英明、小澤岳昌、藤田克昌
    • Organizer
      第39回日本分子生物学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Single molecule imaging in living cells to reveal the relationship between motions and functions of biological molecules2016

    • Author(s)
      吉村英哲,小澤岳昌
    • Organizer
      The 10th International Symposium on Nanomedicine (ISNM2016)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of cellular signaling manipulation method utilizing a blue light bioluminescence2016

    • Author(s)
      河村玄気,桂嘉宏,小澤岳昌
    • Organizer
      The 10th International Symposium on Nanomedicine (ISNM2016)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of photo-activatable TNFR12016

    • Author(s)
      児玉祐来,遠藤瑞己,吉村英哲,小澤岳昌
    • Organizer
      The 10th International Symposium on Nanomedicine (ISNM2016)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of UV-C evoked circadian clock synchronization mechanism by a single-cell bioluminescence imaging2016

    • Author(s)
      河村玄気,服部満,田丸輝也,小澤岳昌
    • Organizer
      International Conference on Single Cell Research 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of a novel SUMOylated protein using split fluorescence protein fragments2016

    • Author(s)
      小宮麻希,比留間大祐,服部満,小澤岳昌
    • Organizer
      International Conference on Single Cell Research 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic Control of Endocytosis and Trafficking of Ligand-activatable GPCR2016

    • Author(s)
      竹之内修,吉村英哲,小澤岳昌
    • Organizer
      International Conference on Single Cell Research 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞膜上受容体 TNFR1 の光制御モジュール開発2016

    • Author(s)
      児玉祐来,遠藤瑞己,吉村英哲,小澤岳昌
    • Organizer
      第6回 CSJ化学フェスタ2016
    • Related Report
      2016 Annual Research Report
  • [Presentation] Optogenetic Control of GPCR Endocytosis and Trafficking2016

    • Author(s)
      竹之内修,吉村英哲,小澤岳昌
    • Organizer
      8th Summer School Medicinal Chemistry in Regensburg
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic Control of Ligand-activating GPCR Endocytosis and Trafficking2016

    • Author(s)
      竹之内修,吉村英哲,小澤岳昌
    • Organizer
      RSC Tokyo International Conference 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of real-time motion of signal transduction molecule Akt in living cells to reveal its functioning mechanism2016

    • Author(s)
      吉村英哲,山田俊理,小澤岳昌
    • Organizer
      RSC Tokyo International Conference 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a Bioluminescent Probe Based on Split Luciferase Complementation Assay for in Vivo Optical Imaging2016

    • Author(s)
      Li Qiaojing, 小澤岳昌
    • Organizer
      FB3 2016 (Fluorescent Biomolecules and Their Building Blocks)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光依存的タンパク質間相互作用を用いた生体内における軸索誘導技術の開発2016

    • Author(s)
      遠藤瑞己,服部満,小澤岳昌
    • Organizer
      日本ケミカルバイオロジー学会第11回年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 化学物質のエストロゲン活性を高感度かつ迅速に評価可能な発光インジケー タの開発2016

    • Author(s)
      竹之内 修, 菅野 憲, 高倉 栄男, 小澤 岳昌
    • Organizer
      日本ケミカルバイオロジー学会第11回年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      PACIFICHEM2015
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      Asian-CHIP2015
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Optical control of extending neurite direction with light-induced protein oligomerizing system2015

    • Author(s)
      遠藤瑞己・服部満・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Imaging system for monitoring of intracellular acidification in living tissues by photo-controllable luciferase2015

    • Author(s)
      服部満・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualising stem cell differentiation status by Raman micro-spectroscopy2015

    • Author(s)
      Liang-da Chiu, Taro Ichimura, Fujita Katsumasa, Takeaki Ozawa, Hideaki Fujita
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of molecular mechanisms underlying ultraviolet-C induced circadian clock synchronization using luciferase probes.2015

    • Author(s)
      河村玄気・服部満・田丸輝也・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bioluminescence analysis in living cells using NanoLuc luciferase based probes2015

    • Author(s)
      服部満・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Separation of Raman and Fluorescence Signals by Wavelength Modulation Raman Spectroscopy with Parallel Accumulation2015

    • Author(s)
      Rintaro Shimada, Takashi Nakamura, Takeaki Ozawa
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a genetically-encoded probe to visualize β-actin mRNA in living cells based on a reconstituted GFP and an RNA binding domain PUM-HD2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Takeaki Ozawa
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of red-shifted luciferase mutants derived from Brazilian click beetle Pyrearinus termitilluminans2015

    • Author(s)
      西口知輝・山田俊理・伊藤真志保・那須雄介・吉村英哲・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Live cell imaging and in vivo analysis for temporal reaction of G protein-coupled receptor using split luciferase complementation2015

    • Author(s)
      服部満・小澤岳昌
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A fluorescent probe for single-molecule live-cell imaging of telomeric-repeat containing RNA using fluorescent protein reconstitution and an RNA-binding domain PUM-HD2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Takeaki Ozawa
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii(USA)
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single molecule imaging of telomeric-repeat containing RNA in living cells2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Takeaki Ozawa
    • Organizer
      9th International Symposium on Nanomedicine
    • Place of Presentation
      三重大学(三重県津市)
    • Year and Date
      2015-12-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Integral roles of heat-shock response pathway in UV-C irradiation-evoked circadian clock synchronization process.2015

    • Author(s)
      河村玄気・田丸輝也・服部満・高松研・小澤岳昌
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際展示場,兵庫県
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] タンパク質光多量化反応を用いた生体内における軸索伸長制御法の開発2015

    • Author(s)
      遠藤瑞己・服部満・小澤岳昌
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生きたまま分子機能を捉え・操るーオプトバイオアナリシス2015

    • Author(s)
      小澤岳昌
    • Organizer
      第246回生理学東京談話会
    • Place of Presentation
      東邦大学医学部(東京都大田区)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] CRYが駆動する哺乳類時計蛋白質キナーゼの日周活性振動2015

    • Author(s)
      田丸輝也・服部満・中畑泰和・小澤岳昌・高松研
    • Organizer
      第22回日本時間生物学会学術大会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2015-09-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生きた細胞内の分子を観る・操作する新たな光技術2015

    • Author(s)
      小澤岳昌、吉村英哲、山田俊理
    • Organizer
      高分子学会バイオ・高分子研究会
    • Place of Presentation
      仙台秋保岩沼屋(宮城県仙台市)
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Single molecule imaging of telomeric repeat containing RNA in living cells2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Hiroki Segawa, Takeaki ozawa
    • Organizer
      第53回生物物理学会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生細胞発光イメージングを利用したハイスループット解析システムの開発2015

    • Author(s)
      服部満・小澤岳昌
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] タンパク質光多量化モジュールを用いた光による軸索伸長制御2015

    • Author(s)
      遠藤瑞己・服部満・小澤岳昌
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] テロメアRNA生細胞内1分子動態の定量評価法2015

    • Author(s)
      吉村英哲、山田俊理、瀬川尋貴、小澤岳昌
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Investigation of a bioluminescent probe to quantify the activity of photoreceptors2015

    • Author(s)
      西口知輝・小澤岳昌
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of luciferase probes for the elucidation of synchronization mechanism of circadian clock by ultraviolet-C irradiation.2015

    • Author(s)
      河村玄気・服部満・田丸輝也・小澤岳昌
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of a novel SUMOylated protein and analysis of the SUMOylation function2015

    • Author(s)
      小宮麻希・比留間大祐・服部満・小澤岳昌
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a specific binding scaffold repebody targeting clathrin2015

    • Author(s)
      Kazuki Fukuda, Kazuma Eto, Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of dynamics on a non-coding RNA in living cells using a single molecule tracking approach2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Hiroki Segawa, Takeaki Ozawa
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2015-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      OIIB Summer School 2015
    • Place of Presentation
      岡崎統合バイオサイエンスセンター(愛知県岡崎市)
    • Year and Date
      2015-08-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生体分子の時空間制御とライブイメージング2015

    • Author(s)
      小澤岳昌
    • Organizer
      イメージングブートキャンプ
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2015-06-17
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 高速励起波長変調によるラマン散乱と蛍光の分離の試み2015

    • Author(s)
      中村昂・島田林太郎・小澤岳昌
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨大学(山梨県甲府市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 細胞内1分子動態分析に基づくテロメアRNAの作動機構解析法2015

    • Author(s)
      吉村英哲、山田俊理、瀬川尋貴、小澤岳昌
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨大学(山梨県甲府市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Protein-based luminescent sensors for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      The 3rd international symposium on molecular imaging and nanomedicine
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2015-04-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and analysis of biomolecules in living cells2015

    • Author(s)
      T. Ozawa,
    • Organizer
      PITTCON 2015
    • Place of Presentation
      New Orleans (USA)
    • Year and Date
      2015-03-10
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors for single cell analysis2015

    • Author(s)
      T. Ozawa
    • Organizer
      International Symposium on Multi-dimensional Fluorescence Live Imaging of Cellular Functions and Molecular Activities
    • Place of Presentation
      京都国際会館(京都・京都)
    • Year and Date
      2015-01-28
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors for single cell analysis2014

    • Author(s)
      T. Ozawa
    • Organizer
      Biomedical Molecular Imaging 2014
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2014-11-08
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 二分割ルシフェラーゼ再構成法を用いた生理機能解析法2014

    • Author(s)
      小澤岳昌
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都・京都)
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Luminescent sensors and optical switches for single cell analysis2014

    • Author(s)
      T. Ozawa,
    • Organizer
      Shino-Japan Workshop on Chemical Biology
    • Place of Presentation
      Beijing (China)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Luminescent sensors and optical switches for single cell analyses2014

    • Author(s)
      T. Ozawa
    • Organizer
      Swiss-Japan Chemical Biology Symposium 2014
    • Place of Presentation
      Bern (Swiss)
    • Year and Date
      2014-10-03
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生きた細胞内で機能する内在性RNAイメージング法2014

    • Author(s)
      小澤岳昌、吉村英哲、山田俊理
    • Organizer
      分子研研究会(細胞核内反応の分子科学)
    • Place of Presentation
      岡崎コンファレンスセンター(愛知・岡崎)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Visualising and Manipulating Cell Stimulation2014

    • Author(s)
      T. Ozawa
    • Organizer
      8th Pituitary Meeting
    • Place of Presentation
      Edinburgh (UK)
    • Year and Date
      2014-09-22
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging and Analysis of GPCRs using Protein Complementation Analysis2014

    • Author(s)
      T. Ozawa,
    • Organizer
      7th Summer School Medicinal Chemistry in Regensburg
    • Place of Presentation
      Regensburg (Germany)
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生細胞内の生理機能を探る光分析技術2014

    • Author(s)
      小澤岳昌
    • Organizer
      第31回東北大学無機・分析化学コロキウム
    • Place of Presentation
      東北大学(宮城・仙台)
    • Year and Date
      2014-05-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] 発光イメージング実験ガイド2019

    • Author(s)
      永井健治,小澤岳昌
    • Total Pages
      222
    • Publisher
      羊土社
    • ISBN
      9784758122405
    • Related Report
      2018 Annual Research Report
  • [Book] Optogenetics2018

    • Author(s)
      Sophie Vriz and Takeaki Ozawa
    • Total Pages
      225
    • Publisher
      oyal Society of Chemistry
    • ISBN
      9781788012379
    • Related Report
      2018 Annual Research Report
  • [Book] スクーグ分析化学2018

    • Author(s)
      小澤岳昌
    • Total Pages
      414
    • Publisher
      東京化学同人
    • ISBN
      9784807908707
    • Related Report
      2018 Annual Research Report
  • [Remarks] プレス発表

    • URL

      http://www.chem.s.u-tokyo.ac.jp/users/analyt/pickup.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 受賞関連

    • URL

      http://www.chem.s.u-tokyo.ac.jp/users/analyt/award.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] セミナー(アウトリーチ活動)

    • URL

      http://www.chem.s.u-tokyo.ac.jp/users/analyt/seminar.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2014-06-05   Modified: 2023-03-23  

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