| Budget Amount *help |
¥39,000,000 (Direct Cost: ¥30,000,000、Indirect Cost: ¥9,000,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2014: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Outline of Final Research Achievements |
We assumed that the interaction of chromatin remodeling factors with nucleosomes would involve local detachments of the nucleosomal DNA from histones during DNA transaction. Therefore, we studied the interaction of chromatin remodeling factors with nicked nucleosomes by biochemical and structural analyses. As a result, the introduction of a double-strand break (DSB) into the nucleosomal DNA allows a histone chaperone FACT to efficiently form a complex with nucleosomes. It is thus indicated that DNA cleavages on nucleosomes would cause local unwrapping of the nucleosomal DNA in the vicinity of the cleavage sites upon contacting FACT, leading to subsequent reactions for chromatin remodeling.
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