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Ecogenomics studies for the genetic basis of adaptive speciation

Research Project

Project/Area Number 26251044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionKyoto University

Principal Investigator

Sota Teiji  京都大学, 理学研究科, 教授 (00192625)

Co-Investigator(Renkei-kenkyūsha) NIIMI Teruyuki  基礎生物学研究所, 進化発生研究部門, 教授 (00293712)
Research Collaborator OKUZAKI Yutaka  
FUJISAWA Tomochika  
KOMURAI Ryohei  
SUGAWARA Hisashi  
NOMURA Shota  
Project Period (FY) 2014-06-27 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Keywords種分化 / 生殖隔離 / 適応進化 / 体サイズ / 交尾器形態 / ゲノム解読 / QTL解析 / オサムシ / 交尾器 / 遺伝的基盤 / 適応的形態 / ゲノム配列 / 適応的種分化 / 生態ゲノミクス / 遺伝的連鎖地図
Outline of Final Research Achievements

Revealing the genetic basis of traits for reproductive isolation based on the analysis of the whole genome is an important subject in evolutionary ecology. In Carabus (Ohomopterus) beetles, differences in genital morphology and body size contribute to pre-zygotic reproductive isolation, and diversification in these traits contributes to species the richness and formation of multi-species assembly. We sequenced the whole genome of representative species and searched for QTLs and candidate genes for species-specific genital morphology and body size differences. For genital morphology, we investigated QTLs for species-specific genital dimensions between C. iwawakianus and C. maiyasanus, referring to the whole genome sequence of C. uenoi. For body size, we investigated QTLs for body size dimensions using a crossing between two populations in C. japonicus. For both traits, we found a few QTLs with large phenotypic effects and revealed candidate genes located in the QTL regions.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (11 results)

All 2018 2017 2016 2015 2014

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (4 results)

  • [Journal Article] Predator size divergence depends on community context2018

    • Author(s)
      Okuzaki, Y., Sota, T.
    • Journal Title

      Ecology Letters

      Volume: in press Issue: 7 Pages: 1097-1107

    • DOI

      10.1111/ele.12976

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic regions and genes related to inter-population differences in body size in the ground beetle Carabus japonicus2017

    • Author(s)
      Komurai, R., Fujisawa, T., Okuzaki, Y., Sota, T.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 7773-7773

    • DOI

      10.1038/s41598-017-08362-7

    • NAID

      120006817598

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Factors related to altitudinal body size variation in the earthworm-eating ground beetle Carabus japonicus2017

    • Author(s)
      Okuzaki, Y., Sota, T.
    • Journal Title

      Zoological Science

      Volume: 34 Issue: 3 Pages: 229-234

    • DOI

      10.2108/zs160182

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Factors Related to Altitudinal Body Size Variation in the Earthworm-eating Ground Beetle Carabus japonicus2017

    • Author(s)
      Okuzaki, Y. and Sota, T.
    • Journal Title

      Zoological Science

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Body size evolution under character release in the ground beetle Carabus japonicus2015

    • Author(s)
      Okuzaki Y, Sugawara H, Sota T
    • Journal Title

      Journal of Biogeography

      Volume: 42 Issue: 11 Pages: 2145-2158

    • DOI

      10.1111/jbi.12575

    • NAID

      120005859702

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Comparative transcriptomic analysis of two closely related ground beetle species with marked genital divergence using pyrosequencing2014

    • Author(s)
      Fujimaki, K., T. Fujisawa, S. Yazawa, O. Nishimura & T. Sota
    • Journal Title

      Zoological Science

      Volume: 31 Issue: 9 Pages: 587-592

    • DOI

      10.2108/zs140081

    • NAID

      40020183276

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Application of RAD-based phylogenetics to complex relationships among variously related taxa in a species flock2014

    • Author(s)
      Takahashi, T., N. Nagata & T. Sota
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 80 Pages: 137-144

    • DOI

      10.1016/j.ympev.2014.07.016

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 群集構造に依存した捕食者の体サイズ分化:気候・餌サイズ・近縁種の相対的重要性2018

    • Author(s)
      奥崎穰、曽田貞滋
    • Organizer
      日本生態学会第65回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 種特異的交尾器形態をもつオオオサムシ亜属における遺伝子発現比較2017

    • Author(s)
      野村翔太,曽田貞滋
    • Organizer
      日本進化学会第19回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 餌サイズと繁殖干渉を淘汰圧としたヒメオサムシの体サイズ分化2016

    • Author(s)
      奥崎穣,曽田貞滋
    • Organizer
      第63回生日本態学会大会
    • Place of Presentation
      仙台市 仙台国際センター
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Distance decay of similarity in fungivorous insect communities: assessing dispersal limitation using genetic data2015

    • Author(s)
      曽田貞滋
    • Organizer
      日本昆虫学会第75回大会
    • Place of Presentation
      福岡市 九州大学
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report

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Published: 2014-11-20   Modified: 2019-03-29  

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