Search for innovative diagnostic and therapeutic seeds based on next generation OMICS analysis using new disease models

• Project/Area Number: 26253078
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: Ogawa Osamu 京都大学, 医学研究科, 教授 (90260611)
• Co-Investigator(Kenkyū-buntansha): 井上 貴博 京都大学, 医学(系)研究科(研究院), 助教 (80511881) ; 根来 宏光 京都大学, 医学研究科, 助教 (80708595) ; 神波 大己 京都大学, 医学研究科, 准教授 (20402836) ; 松井 喜之 京都大学, 医学(系)研究科(研究院), 助教 (00582107)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥39,000,000 (Direct Cost: ¥30,000,000、Indirect Cost: ¥9,000,000); Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2014: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
• Keywords: omics / 質量分析 / 前立腺癌 / 腎癌 / xenograft / 癌 / プロテオミクス / 治療抵抗性 / バイオマーカー

Outline of Final Research Achievements

We have established patient-derived tumor tissue (PDTT) xenograft models of urological cancer. Those models show diversity in response to treatment like clinical patients. We have analyzed tumor tissues and body fluid of the PDTT xenograft models by next generation high sensitive mass spectrometry, and identified new molecules related to cancer malignancy and treatment resistance. We are planning to evaluate whether those molecules could be biomarkers or therapeutic targets in clinical setting.

Research Products

• [Journal Article] An original patient-derived xenograft of prostate cancer with cyst formation. (2016)
  • Author(s): Takeshi Yoshikawa
  • Journal Title: The Prostate Volume: 76 Issue: 11 Pages: 994-1003
  • DOI: 10.1002/pros.23188
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma. (2015)
  • Author(s): 柴崎 昇
  • Journal Title: Plos One Volume: 10 Issue: 6 Pages: 1-20
  • DOI: 10.1371/journal.pone.0130980
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Up-regulation of miR-582-5p regulates cellular proliferation of prostate cancer cells under androgen-deprived conditions. (2014)
  • Author(s): 前野淳
  • Journal Title: Prostate Volume: 74 Issue: 16 Pages: 1604-12
  • DOI: 10.1002/pros.22877
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] LCMSを用いた前立腺癌細胞株のリン脂質発現解析 (2018)
  • Author(s): 岡所広祐 小川修 ら
  • Organizer: 第27回泌尿器科分子細胞研究会
• [Presentation] 淡明型腎細胞癌におけるホスファチジン酸の検討 (2018)
  • Author(s): 宇都宮紀明 小川修 ら
  • Organizer: 第27回泌尿器科分子細胞研究会
• [Presentation] An original patient-derived xenograft model for castration-resistant prostate cancer expressing AR-V7 (2017)
  • Author(s): 牧野雄樹 小川修 ら
  • Organizer: 第105回日本泌尿器科学会総会
• [Presentation] An analysis of phosphatidic acid for clear cell renal cell carcinoma (2017)
  • Author(s): 宇都宮紀明 小川修 ら
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] An analysis of renal cell carcinoma (RCC) xenograft models developing resistance to axitinib (2016)
  • Author(s): 宇都宮紀明
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] An analysis of renal cell carcinoma (RCC) xenograft models developing resistance to axitinib (2016)
  • Author(s): 宇都宮紀明
  • Organizer: 第14回アジア泌尿器科学会
  • Place of Presentation: シンガポール
  • Year and Date: 2016-07-19
  • Int'l Joint Research
• [Presentation] Primary xenograftを用いた腎細胞癌アキシチニブ耐性獲得機序の解明 (2016)
  • Author(s): 宇都宮紀明
  • Organizer: 第104回日本泌尿器科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-04-22
• [Presentation] Primary Xenograftを用いた腎細胞癌アキシチニブ耐性獲得機序の解明 (2016)
  • Author(s): 宇都宮 紀明
  • Organizer: 第25回泌尿器科分子・細胞研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-02-26
• [Presentation] マイクロRNA miR-582-5pはアンドロゲン除去下での前立腺癌細胞増殖を制御する (2015)
  • Author(s): 前野 淳
  • Organizer: 第103回日本泌尿器科学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2015-04-18
• [Presentation] IL13RA2 mediates Resistance to Sunitinib in Primary Xenograft Models of Clear Cell Renal Cell Carcinoma (2014)
  • Author(s): 柴崎 昇
  • Organizer: The 31st Japan-Korea Urological Congress
  • Place of Presentation: 東京
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Investigation of CCL2 as a potential therapeutic target in clear cell renal cell carcinoma (2014)
  • Author(s): 新垣隆一郎
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] ヒト前立腺小細胞癌由来のゼノグラフトマウスモデルの樹立 (2014)
  • Author(s): 植垣正幸
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] スニチニブ感受性に関係する腫瘍および間質由来遺伝子変化の探索 (2014)
  • Author(s): 柴崎 昇
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2014-04-24 – 2014-04-27
• [Presentation] 腎細胞癌に対する新規治療ターゲットとしてのCCL2の検討 (2014)
  • Author(s): 新垣隆一郎
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2014-04-24 – 2014-04-27