Functional analysis of the novel RING-finger domain containing Fanconi anemia protein
| Project/Area Number |
26281021
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Risk sciences of radiation and chemicals
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Ishiai Masamichi 京都大学, 放射線生物研究センター, 准教授 (90298844)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
|
|---|
| Keywords | DNA修復 / ユビキチン / ファンコニ貧血 / 相同組換え / RPA / RAD51 / RFWD3 / FANCW / 環境 / 放射線 |
|---|
| Outline of Final Research Achievements |
Fanconi anemia (FA) is a hereditary disorder defective in DNA interstrand crosslinks (ICL) and characterized by developmental anomalies, progressive bone marrow failure, leukemia, and solid tumors.
RFWD3 is a recently identified FA protein FANCW whose ubiquitin E3 ligase activity toward RPA is essential in homologous recombination (HR) repair. However, how RPA ubiquitination promotes HR remained unknown. We identified RAD51, the central HR protein, as another target of RFWD3. We show that RFWD3 polyubiquitinates both RPA and RAD51, in vitro and in vivo. From further analysis, our data reveal a mechanism that facilitates timely removal of RPA and RAD51 from DNA damage sites, which is crucial for progression to the late-phase HR.
|
Report
(4 results)
Research Products
(20 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Novel Fanconi anemia E3 ligase RFWD3 promotes removal of both RPA and RAD51 from DNA damage sites during ICL repair.2016
Author(s)
Shojiro Inano, Koichi Sato, Kerstin Knies, Yoko Katsuki, Shinichiro Nakada, Akifumi Takaori-Kondo, Masamicih Ishiai, Detlev Schindler, Hitoshi Kurumizaka, Minoru Takata
Organizer
8th Annual Fanconi Anemia research frund Scientific Symposium
Place of Presentation
Bellevue, WA USA
Year and Date
2016-09-15
Related Report
Int'l Joint Research
-
[Presentation] Novel Fanconi anemia E3 ligase RFWD3 regulates RPA and RAD51 degradation to facilitate homologous recombination and ICL repair.2016
Author(s)
Shojiro Inano, Koichi Sato, Yoko Katsuki, Shinichiro Nakada, Akifumi-Takaori0Kondo, Masamichi Ishiai, Detlev Schindler, Hitoshi Kurumizaka, Minoru Takata
Organizer
DNA metablism, Genomic stability, & Diseases. The 2016 Cold Spring Harbor Asia Conference
Place of Presentation
Suzhou, China
Year and Date
2016-06-13
Related Report
Int'l Joint Research
-
-
[Presentation] The Roles of FANCD2, a central player of the Fanconi anemia pathway, in DNA repair2015
Author(s)
Ishiai, M., Sato, K., Kurumizaka, H., and Takata, M.
Organizer
15th International Congress of Radiation Research (ICRR2015)
Place of Presentation
Kyoto, JAPAN
Year and Date
2015-05-25
Related Report
Int'l Joint Research
-
-
-
-
