A mechanism for chromosome instability mediated by micronuclei induced with radiation
| Project/Area Number |
26281024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KODAMA Seiji 大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)
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| Co-Investigator(Kenkyū-buntansha) |
杉本 憲治 大阪府立大学, 生命環境科学研究科(系), 教授 (40196746)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAISHI Kazunori 大阪府立大学, 大学院理学系研究科, 助教 (40347513)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 染色体不安定化 / 放射線 / 小核形成 / ライブセルイメージング / DNA2本鎖切断 / 生物影響 / 小核 / DNA２本鎖切断 / クロモスリプシス |
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| Outline of Final Research Achievements |
We examined the induction of micronuclei by X-irradiation and their fate during several cell divisions post-irradiation using live cell imaging, which traced living cell lineages. Radiation promoted the induction of a whole chromosome-derived micronucleus(MN) that was mediated by chromosome segregation errors. Further, a whole chromosome-derived MN accumulated DNA double strand breaks (DSBs) during the S phase of main nuclei, and some of them were reincorporated into the main nuclei. The present study indicates the possibility that the chromosome-derived MN, which accumulates DSBs induced by radiation, is incidentally reincorporated into the main nuclei and this process causes the complicated chromosome rearrangements.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Cyclized Helix-Loop-Helix Peptide as a Molecular Scaffold for the Design of Intracellular Protein-Protein Interaction Inhibitors by Epitope and Arginine Grafting2016
Author(s)
Daisuke Fujiwara, Hidekazu Kitada, Masahiro Oguri, Toshio Nishihara, Masataka Michigami, Kazunori Shiraishi, Eiji Yuba, Ikuhiko Nakase, Haeri Im, Sunhee Cho, Jong Young Joung, Seiji Kodama, Kenji Kono, Sihyun Ham*, and Ikuo Fujii*
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Journal Title
Angewandte Chemie International Edition
Volume: 55
Issue: 36
Pages: 10612-10615
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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