| Project/Area Number |
26281024
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KODAMA Seiji 大阪府立大学, 理学(系)研究科(研究院), 教授 (00195744)
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| Co-Investigator(Kenkyū-buntansha) |
杉本 憲治 大阪府立大学, 生命環境科学研究科(系), 教授 (40196746)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAISHI Kazunori 大阪府立大学, 大学院理学系研究科, 助教 (40347513)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 染色体不安定化 / 放射線 / 小核形成 / ライブセルイメージング / DNA2本鎖切断 / 生物影響 / 小核 / DNA２本鎖切断 / クロモスリプシス |
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| Outline of Final Research Achievements |
We examined the induction of micronuclei by X-irradiation and their fate during several cell divisions post-irradiation using live cell imaging, which traced living cell lineages. Radiation promoted the induction of a whole chromosome-derived micronucleus(MN) that was mediated by chromosome segregation errors. Further, a whole chromosome-derived MN accumulated DNA double strand breaks (DSBs) during the S phase of main nuclei, and some of them were reincorporated into the main nuclei. The present study indicates the possibility that the chromosome-derived MN, which accumulates DSBs induced by radiation, is incidentally reincorporated into the main nuclei and this process causes the complicated chromosome rearrangements.
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