New interaction of oxygen binding protein mediating mitochondrial function in skeletal muscle
| Project/Area Number |
26282183
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Kanazawa University |
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Principal Investigator |
Masuda Kazumi 金沢大学, 人間科学系, 教授 (50323283)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAKURA HISASHI 同志社大学, スポーツ健康科学部, 助教 (20631914)
IWANAKA NOBUMASA 立命館大学, グローバルイノベーション研究機構, 研究員 (80584002)
HASHIMOTO TAKESHI 立命館大学, スポーツ健康科学部, 准教授 (70511608)
HAYANO TOSHIYA 立命館大学, 生命科学部, 教授 (90332303)
KATO YUKIO 金沢大学, 薬学系, 教授 (30251440)
HANAI YOSHITERU 名古屋工業大学, 工学研究科, 准教授 (50360730)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 筋細胞 / 代謝 / ミトコンドリア / ミオグロビン / 運動 |
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| Outline of Final Research Achievements |
The present study focused on physiological interaction of myoglobin (Mb) with mitochondria in myocyte. In order to identify the hypothesis, we established Mb overexpressing cultured cell model. The Mb-overexpressing myocytes consumed oxygen faster and showed higher enzymatic activity especially in activity of complex-IV. The Mb-siRNAs transfection canceled these positive effects observed in Mb-overexpressed myocytes. We further tested about Mb localization by mitochondrial fractionation assay involved serial centrifuges. The Mb was, then, observed in the mitochondrial intermembrane space. These evidences suggest that Mb would be one of the factors underlying the mechanisms to regulate muscle mitochondrial respiration through an interaction with mitochondrial complex.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] Pgc-1αの発現抑制がミオグロビン発現量に及ぼす影響について2014
Author(s)
高倉久志, 田中誠智, 田中剛貴, 加藤久詞, 柴原拓哉, 増田慎也, 岩中伸壮, 北條達也, 稗田睦子, 井澤鉄也, 増田和実
Organizer
第69回日本体力医学会大会
Place of Presentation
長崎大学, 長崎
Year and Date
2014-09-19 – 2014-09-21
Related Report
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[Presentation] PGC-1α expression is not essential for endurance training induced increase in myoglobin expression in skeletal muscle2014
Author(s)
Takakura H, Tanaka G, Tanaka S, Kato H, Shibahara T, Masuda S, Iwanaka N, Hata T, Izawa T, Jue T and Masuda K
Organizer
American College of Sports Medicine 61st Annual Meeting
Place of Presentation
Orland, FL, USA
Year and Date
2014-05-27 – 2014-05-31
Related Report
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