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New interaction of oxygen binding protein mediating mitochondrial function in skeletal muscle

Research Project

Project/Area Number 26282183
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Sports science
Research InstitutionKanazawa University

Principal Investigator

Masuda Kazumi  金沢大学, 人間科学系, 教授 (50323283)

Co-Investigator(Renkei-kenkyūsha) TAKAKURA HISASHI  同志社大学, スポーツ健康科学部, 助教 (20631914)
IWANAKA NOBUMASA  立命館大学, グローバルイノベーション研究機構, 研究員 (80584002)
HASHIMOTO TAKESHI  立命館大学, スポーツ健康科学部, 准教授 (70511608)
HAYANO TOSHIYA  立命館大学, 生命科学部, 教授 (90332303)
KATO YUKIO  金沢大学, 薬学系, 教授 (30251440)
HANAI YOSHITERU  名古屋工業大学, 工学研究科, 准教授 (50360730)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords筋細胞 / 代謝 / ミトコンドリア / ミオグロビン / 運動
Outline of Final Research Achievements

The present study focused on physiological interaction of myoglobin (Mb) with mitochondria in myocyte. In order to identify the hypothesis, we established Mb overexpressing cultured cell model. The Mb-overexpressing myocytes consumed oxygen faster and showed higher enzymatic activity especially in activity of complex-IV. The Mb-siRNAs transfection canceled these positive effects observed in Mb-overexpressed myocytes. We further tested about Mb localization by mitochondrial fractionation assay involved serial centrifuges. The Mb was, then, observed in the mitochondrial intermembrane space. These evidences suggest that Mb would be one of the factors underlying the mechanisms to regulate muscle mitochondrial respiration through an interaction with mitochondrial complex.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Remarks (3 results)

  • [Int'l Joint Research] University of California Davis(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Intracellular oxygen tension limits muscle contraction- induced change in muscle oxygen consumption under hypoxic conditions during Hb-free perfusion.2017

    • Author(s)
      214.Takakura H, Ojino M, Jue T, Yamada T, Furuichi Y, Hashimoto T, Iwase S, Masuda K:
    • Journal Title

      Physiological Reports

      Volume: 5 Issue: 2 Pages: 1-10

    • DOI

      10.14814/phy2.13112

    • NAID

      120006226796

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 運動を模擬した薬理刺激による骨格筋培養細胞のミトコンドリア生合成とミオグロビンの発現変化2017

    • Author(s)
      町野綾香, 石澤里枝, 芝口翼, 増田和実
    • Journal Title

      北陸体育学会紀

      Volume: 53

    • NAID

      40021217895

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Myoglobin and the Regulation of Mitochondrial Respiratory Chain Complex IV2016

    • Author(s)
      Yamada T, Takakura H, Jue T, Hashimoto T, Ishizawa R, Furuichi Y, Kato Y, Iwanaka N, Masuda K
    • Journal Title

      The Journal of Physiology

      Volume: 594 Issue: 2 Pages: 483-495

    • DOI

      10.1113/jp270824

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Curcumin treatment enhances the effect of exercise on mitochondrial biogenesis in skeletal muscle by increasing cAMP levels2015

    • Author(s)
      Hamidie DRR, Yamada T, Ishizawa R, Saito Y and Masuda K
    • Journal Title

      Metabolism

      Volume: 64 Issue: 10 Pages: 1334-1347

    • DOI

      10.1016/j.metabol.2015.07.010

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Endurance training facilitates myoglobin desaturation during muscle contraction in rat skeletal muscle.2015

    • Author(s)
      Takakura H., Furuichi Y., Yamada T., Jue T., Ojino M., Hashimoto T., Iwase S., Hojo T., Izawa T. and Masuda K
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 9403-9403

    • DOI

      10.1038/srep09403

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 脂肪酸による骨格筋のミトコンドリア生合成機構2015

    • Author(s)
      石澤里枝, 山田達也, Hamidie DR Ronald, 増田和実
    • Journal Title

      北陸体育学会紀要

      Volume: 51 Pages: 41-54

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Mitochondrial biogenesis induced by exercise and nutrients: implication for performance and health benefits2016

    • Author(s)
      Masuda K
    • Organizer
      The International Conference on Sport Science, Health, and Physical Education 2016
    • Place of Presentation
      Bandung, West Java, Indonesia
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 骨格筋のミトコンドリア呼吸機能とミオグロビン2016

    • Author(s)
      増田和実
    • Organizer
      第24回日本運動生理学会大会(シンポジウム講演)
    • Place of Presentation
      熊本大学, 熊本
    • Year and Date
      2016-07-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Myoglobin regulates mitochondrial respiration through its interaction with complex IV2016

    • Author(s)
      Masuda K, Yamada T, Takakura H, Hashimoto T, Ishizawa R, Furuichi Y, Kato Y, Iwanaka N and Jue T
    • Organizer
      European College of Sport Science 21st Annual Congress
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-07-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Curcumin decreases phosphorylation phosphodiesterase (PDE) to regulated mitochondrial biogenesis in rat skeletal muscle2015

    • Author(s)
      Hamidie DRR, Yamada T, Ishizawa R, Saito Y and Masuda K
    • Organizer
      第70回日本体力医学会大会
    • Place of Presentation
      和歌山県民文化会館, 和歌山
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミトコンドリア呼吸におけるミオグロビン分子の役割2015

    • Author(s)
      増田和実, 山田達也, Hamidie DRR, 石澤里枝
    • Organizer
      第70回日本体力医学会大会
    • Place of Presentation
      和歌山県民文化会館, 和歌山
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ミトコンドリアに対するミオグロビンの機能的相互作用2015

    • Author(s)
      増田和実, 山田達也, Hamidie DRR, 石澤里枝, 齊藤陽子
    • Organizer
      第19回酸素ダイナミクス研究会
    • Place of Presentation
      東京女子医科大学, 東京
    • Year and Date
      2015-09-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] ケトン体による骨格筋のミトコンドリア関連タンパク質増加作用2015

    • Author(s)
      石澤里枝, 山田達也, Hamidie DR Ronald, 増田和実
    • Organizer
      日本スポーツ栄養学会第2回大会
    • Place of Presentation
      立命館大学, 草津
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Endurance training with curcumin treatment regulated mitochondrial biogenesis in skeletal muscle through SIRT1-mediated signaling pathway2015

    • Author(s)
      Hamidie DRR, Ishizawa R, Yamada T and Masuda K
    • Organizer
      American College of Sports Medicine 62nd Annual Meeting
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New insights for the factor regulating muscle mitochondrial respiration2014

    • Author(s)
      Yamada T, Thomas Jue T and Masuda K
    • Organizer
      Cell Symposia System approach to Metabolic Diseases
    • Place of Presentation
      Chicago, IL, USA
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pgc-1αの発現抑制がミオグロビン発現量に及ぼす影響について2014

    • Author(s)
      高倉久志, 田中誠智, 田中剛貴, 加藤久詞, 柴原拓哉, 増田慎也, 岩中伸壮, 北條達也, 稗田睦子, 井澤鉄也, 増田和実
    • Organizer
      第69回日本体力医学会大会
    • Place of Presentation
      長崎大学, 長崎
    • Year and Date
      2014-09-19 – 2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨格筋ミトコンドリアの呼吸調節機序に関わる新規知見2014

    • Author(s)
      山田達也, 石澤里枝, Hamidie DR Ronald, 増田和実
    • Organizer
      第69回日本体力医学会大会
    • Place of Presentation
      長崎大学, 長崎
    • Year and Date
      2014-09-19 – 2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Caffeine treatment stimulates myoglobin synthesis via cAMP signaling in L6 skeletal muscle cells2014

    • Author(s)
      Iwanaka N, Yokokawa T, Fujita T, Masuda K and Hashimoto T
    • Organizer
      ACSM Conference on Integrative Physiology of Exercise
    • Place of Presentation
      Miami Beach, FL, USA
    • Year and Date
      2014-09-17 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Curcumin treatment augment exercise effect on mitochondria biogenesis in skeletal muscle and myocardium2014

    • Author(s)
      Hamidie DRR, Ishizawa R, Yamada T, Nitta S and Masuda K
    • Organizer
      American College of Sports Medicine 61st Annual Meeting
    • Place of Presentation
      Orland, FL, USA
    • Year and Date
      2014-05-27 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enhanced respiration with myoglobin interaction in mitochondria2014

    • Author(s)
      Masuda K, Yamada T and Jue T
    • Organizer
      American College of Sports Medicine 61st Annual Meeting
    • Place of Presentation
      Orland, FL, USA
    • Year and Date
      2014-05-27 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] PGC-1α expression is not essential for endurance training induced increase in myoglobin expression in skeletal muscle2014

    • Author(s)
      Takakura H, Tanaka G, Tanaka S, Kato H, Shibahara T, Masuda S, Iwanaka N, Hata T, Izawa T, Jue T and Masuda K
    • Organizer
      American College of Sports Medicine 61st Annual Meeting
    • Place of Presentation
      Orland, FL, USA
    • Year and Date
      2014-05-27 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Remarks] 金沢大学運動生理学研究室Website

    • URL

      http://exercisephysiol.com

    • Related Report
      2016 Annual Research Report
  • [Remarks] 金沢大学運動生理学・生化学研究室HP(増田研究室)

    • URL

      http://exercisephysiol.com

    • Related Report
      2015 Annual Research Report
  • [Remarks] 金沢大学運動生理学・生化学研究室HP(増田研究室)

    • URL

      http://exercisephysiol.com

    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2022-01-27  

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