| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
We have developed a device and computer programs to measure and analyze ODMR spectra of NV centers in diamond based on an inverted fluorescence microscope, and succeeded in determining the precise orientation of each diamond nanoparticle by applying external magnetic fields sequentially in four inequivalent directions. Diamond nanoparticles were specifically labeled on the EGF receptor via anti-EGF receptor antibody in the cell membrane of A431 cells. By measuring the ODMR spectrum every few minutes and determining the orientation of the diamond nanoparticles, it has become possible to detect the movement of the EGF receptor, that is, the fluctuation motion of the cell membrane. From now on, we will develop this technology to measure various biomolecular dynamics.
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