Development about photochemical detection of nuceic acids by 19F-NMR

• Project/Area Number: 26288077
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: Fujimoto Kenzo 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (90293894)
• Co-Investigator(Kenkyū-buntansha): 坂本 隆 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (80423078)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: １９FーNMR / 核酸検出 / ケミカルシフト / 光架橋反応 / 19F-NMRプローブ / 光クロスリンク / １９F / 核酸検出プローブ / 19F NMR / １９F NMR

Outline of Final Research Achievements

We demonstrated an approach for the chemical shift-based detection of nucleic acids based on CNVK (cyanovinylcarobazole) mediated DNA/RNA photo-cross-linking. The 19F NMR signal of TFT(trifluoromethyl thymidine) in a DNA duplex structure was dramatically shifted from -63.2 ppm to -71.2 ppm induced by photo-cross-linking. This larger chemical shift change induced by CNVK mediated DNA photo-cross-linking enables DNA and RNA detection without confounding effects due to the influence of neighboring bases. We have also successfully demonstrated that 10 nM miRNA can be detected in a sequence-specific manner based on miRNA amplification and chemical shift caused by the photo-cross-linking of CNVK and TFT in DNA and RNA.

Research Products

• [Journal Article] Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using fluorine-modified bisbenzimide derivative (2016)
  • Author(s): Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto
  • Journal Title: Analyst Volume: 141 Issue: 4 Pages: 1214-1217
  • DOI: 10.1039/c5an02389k
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RNA fluorescence in situ hybridization using 3-cyanovinylcarbazole modified oligodeoxyribonucleotides as photo-cross-linkable probes (2016)
  • Author(s): Kenzo Fujimoto, Kei Toyosato, Shigetaka Nakamura, Takashi Sakamoto
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 26 Issue: 21 Pages: 5312-5314
  • DOI: 10.1016/j.bmcl.2016.09.035
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sequence-specific DNA photo-splitting of 3-cyanovinylcarbazole using DNA strand displacement (2016)
  • Author(s): Shigetaka Nakamura, Hayato Kawabata, Kenzo Fujimoto
  • Journal Title: ChemBioChem Volume: 17 Issue: 16 Pages: 1499-1503
  • DOI: 10.1002/cbic.201600236
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of 5-substitution of uracil base in DNA photo-cross-linking using 3-cyanovinylcarbazole (2016)
  • Author(s): Shigetaka Nakamura, Hayato Kawabata, Hodaka Muramatsu, Kenzo Fujimoto
  • Journal Title: Chem. Lett. Volume: 45 Issue: 8 Pages: 887-889
  • DOI: 10.1246/cl.160382
  • NAID: 130005254533
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] UVA Responsive Anticancer Prodrugs Based on Photoinduced Electron Injection into Oligonucleotide Having 5-Halouracils (2016)
  • Author(s): Kenzo Fujimoto, Mirei Furusawa, Shigetaka Nakamura, Takashi Sakamoto
  • Journal Title: Chem. Lett. Volume: 45 Issue: 9 Pages: 1078-1080
  • DOI: 10.1246/cl.160492
  • NAID: 130005549101
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A versatile puromycin-linker using cnvK for high-throughput in vitro selection by cDNA display (2015)
  • Author(s): Yuki Mochizuki, Takeru Suzuki, Kenzo Fujimoto, Naoto Nemoto
  • Journal Title: Journal of Biotechnology Volume: 212 Pages: 174-180
  • DOI: 10.1016/j.jbiotec.2015.08.020
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Critical Effect of Base Pairing of Target Pyrimidine on the Inter-strand Photo-cross-linking of DNA via 3-Cyanovinylcarbazole Nucleoside (2015)
  • Author(s): Takashi Sakamoto, Minako Ooe, Kenzo Fujimoto
  • Journal Title: Bioconjugate Chemistry Volume: 26 Issue: 8 Pages: 1475-1478
  • DOI: 10.1021/acs.bioconjchem.5b00352
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Photo-cross-linking using trifluorothymidine and 3-cyanovinylcarbazole induced large shifted 19F MR signal (2015)
  • Author(s): Shigetaka Nakamura, Kenzo Fujimoto
  • Journal Title: Chemical Communications Volume: 51 Issue: 59 Pages: 11765-11768
  • DOI: 10.1039/c5cc02972d
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fluorine-modified bisbenzimide derivative as a molecular probe for bimodal and simultaneous detection of DNAs by 19F NMR and fluorescence (2015)
  • Author(s): Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto
  • Journal Title: Chemical Communications Volume: 51 Issue: 42 Pages: 8749-8752
  • DOI: 10.1039/c5cc01995h
  • Peer Reviewed
• [Journal Article] DNA Photo-cross-linking using 3-Cyanovinylcarbazole Modified Oligonucleotide with Threoninol Linker (2015)
  • Author(s): Takashi Sakamoto; Yuya Tanaka; Kenzo Fujimoto
  • Journal Title: Organic Letters Volume: 17 Issue: 4 Pages: 936-939
  • DOI: 10.1021/acs.orglett.5b00035
  • Peer Reviewed / Open Access
• [Journal Article] Short oligonucleotide prodrug having 5-fluoro and 5-iodouracil inhibits the proliferation of cancer cells in a photo-responsive manner (2014)
  • Author(s): Kenzo Fujimoto, Yu-ki Takematsu, Atsuo Shigeno, Mirei Furusawa, Takashi Sakamoto
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 24 Issue: 16 Pages: 3736-3738
  • DOI: 10.1016/j.bmcl.2014.07.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Photo-regulation of constitutive gene expression in living cells by using ultrafast photo-cross-linking oligonucleotides (2014)
  • Author(s): Takashi Sakamoto, Atsuo Shigeno, Yuichi Ohtaki, Kenzo Fujimoto
  • Journal Title: Biomaterials Science Volume: 2 Issue: 9 Pages: 1154-1157
  • DOI: 10.1039/c4bm00117f
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Creation of DNA Array Structure Equipped with heat resistance by Ultrafast Photocrosslinking (2014)
  • Author(s): Shigetaka Nakamura, Kenzo Fujimoto
  • Journal Title: Journal of Chemical Technology & Biotechnology Volume: 89 Issue: 7 Pages: 1086-1090
  • DOI: 10.1002/jctb.4205
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Development of novel photo-splitting for photo-cross-linked dsDNA using DNA strand displacement (2017)
  • Author(s): Shigetaka Nakamura, Hayato Kawabata, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-19
• [Presentation] 3-Vinylcarbazole derivatives accelerated photochemical C-U transition in DNA duplex (2017)
  • Author(s): Yasuharu Takashima, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Development of RNA FISH using photoresponsive molecular beacon probe (2017)
  • Author(s): Chinami Kano, Kei Toyosato, Takashi Sakamoto, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Development of novel DNA photo-cross-linker for photochemical DNA manipulation with 400 nm irradiation (2017)
  • Author(s): Shinobu Sasago, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Construction of photoinduced DNA signal release system dependent on ATP concentration (2017)
  • Author(s): Hitoshi Matsuno, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Development of photochemical DNA manipulation toward for the repair of thymine dimer in DNA (2017)
  • Author(s): Ryosuke Jinbo, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Detection of 5-Methylcytosine and cytosine using vinyl-carbazole derivatives mediated DNA photo-cross-linking (2017)
  • Author(s): Ryo Nakajima, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] The effect of counterpart base of target cytosine in 3-cyanovinylcarbazole mediated C → U transition in DNA strand (2017)
  • Author(s): Nozomi Honda, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] 19F chemical shift detection of DNA B-Z transition using fluorine labeled pyrimidine (2017)
  • Author(s): Hui Yang, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Photoinduced double duplex formation mediated by ultrafast DNA photo-cross-linking for antigene method (2017)
  • Author(s): Yang Hung, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Development of novel photoresponsive DNA by postmodification method (2017)
  • Author(s): Li Xue, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-17
• [Presentation] Biomolecule simultaneous analysis by using 19F NMR (2017)
  • Author(s): Qiu Zhiyong, Takashi Sakamoto, Kenzo Fujimoto
  • Organizer: 日本化学会第97春季年会
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] Development of photochemical DNA and RNA manipulation toward for nucleic acid-based drug (2016)
  • Author(s): Kenzo Fujimoto
  • Organizer: The 39th Annual Meeting of Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama（神奈川県横浜市）
  • Year and Date: 2016-12-01
  • Invited
• [Presentation] 光化学的RNA架橋反応を用いた新規FISH法の開発 (2016)
  • Author(s): 中村 重孝、豊里 慧、狩野 千波、藤本 健造
  • Organizer: The 39th Annual Meeting of Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] アンチジーン法に向けた光誘起型Double duplex構造の形成 (2016)
  • Author(s): 中村 重孝、藤本 健造
  • Organizer: 日本核酸医薬学会 第２回年会
  • Place of Presentation: 東京理科大学葛飾キャンパス（ 東京都葛飾区）
  • Year and Date: 2016-11-16
• [Presentation] 高速遺伝子検出へ向けたDNA鎖交換反応の高速化 (2016)
  • Author(s): 高嶋 康晴、中村 重孝、藤本 健造
  • Organizer: 日本核酸医薬学会 第２回年会
  • Place of Presentation: 東京理科大学葛飾キャンパス（ 東京都葛飾区）
  • Year and Date: 2016-11-15
• [Presentation] The effect of 5-substitution in pyrimidine base via DNA photo-cross-linking using 3-cyanovinylcabrazole (2016)
  • Author(s): Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: CBI Annual Meething 2016
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-10-25
• [Presentation] Photochemical RNA editing suign 3-vinylcarbazole analog containing oligodeoxynucleotide (2016)
  • Author(s): Yasuharu Takashima, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: CBI Annual Meething 2016
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-10-25
• [Presentation] The effect of 5-substitution of pyrimidine base in DNA photo-cross-linking using 3-cyanovinylcarbazole (2016)
  • Author(s): Shigetaka Nakamura, Hayato Kawabata, Hodaka Muramatsu, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Detection of DNA B-Z transition by 19F NMR based on 19F chemical shift change (2016)
  • Author(s): Hui Yang, Chihiro Hirata, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] RNA FISH using 3-cyanovinylcarbazole-modified ODNs as photo-cross-linking probe (2016)
  • Author(s): Chinami Kano, Kei Toyosato, Shigetaka Nakamura, Takashi Sakamoto, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Disassembly-driven signal turn-on probe for multimodal detection of DNAs using 19F NMR and Fluorescence (2016)
  • Author(s): Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] C- U transition in DNA duplex using 3-vinylcarbazole analog mediated by reversible photo-cross-linking (2016)
  • Author(s): Yasuharu Takashima, Hirata, Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Effects of hydrogen bonding on the cytosine deamination in photo-cross-linked DNA duplex (2016)
  • Author(s): Siddhant Sethi, Takashi Sakamoto, Kenzo Fujimoto
  • Organizer: The 43rd International Symposium on Nucleic Acids
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] 光化学的な部位特異的核酸塩基編集のためのビニルカルバゾール誘導体の合成 (2016)
  • Author(s): 高嶋 康晴、中村 重孝、藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-08
• [Presentation] シアノビニルカルバゾールを用いたDNA光架橋反応におけるピリミジン5位の置換基効果 (2016)
  • Author(s): 中村 重孝、藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-08
• [Presentation] 改変型 OPA 反応を利用したアミノ酸分析法の開発 (2016)
  • Author(s): QIU Zhiyong、坂本 隆、藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-08
• [Presentation] 高速光架橋能を有する蛍光プローブを用いた RNA FISH (2016)
  • Author(s): 狩野 千波、豊里 慧、中村 重孝、坂本 隆・藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-08
• [Presentation] シアノビニルカルバゾールによって光架橋された DNA 上シトシンの脱アミノ化における水素結合の影響 (2016)
  • Author(s): SETHI Siddhant・SAKAMOTO Takashi・FUJIMOTO Kenzo
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-08
• [Presentation] フッ素ラベル化したピリミジン塩基を用いたDNA B-Z構造転移解析 (2016)
  • Author(s): 楊 卉、平田 千紘、中村 重孝、藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-07
• [Presentation] 光照射をトリガーとしたDNAナノ構造構築 (2016)
  • Author(s): 長谷川 貴司、中村 重孝、藤本 健造
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-07
• [Presentation] フッ素標識ヘキストプローブを用いた19F NMRによるDNA検出 (2016)
  • Author(s): 坂本 隆、長谷川 大策、藤本 健造
  • Organizer: 第27回バイオ高分子シンポジウム
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-07-29
• [Presentation] 光応答性核酸を用いた DNA 鎖交換速度制御とその機構 (2016)
  • Author(s): 中村 重孝、藤本 健造
  • Organizer: 第27回バイオ高分子シンポジウム
  • Place of Presentation: 東京工業大学（東京都目黒区）
  • Year and Date: 2016-07-28
• [Presentation] 高速光架橋能を有するオリゴDNAプローブを用いた新規 FISH 法の開発 (2016)
  • Author(s): 中村重孝、豊里慧、坂本隆、藤本健造
  • Organizer: 第１１回ケミカルバイオロジー学会年会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2016-07-16
• [Presentation] 核酸医薬を指向した光化学的RNA編集法の開発 (2016)
  • Author(s): 藤本健造
  • Organizer: 第55回日本生体医工学会大会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-04-26
  • Invited
• [Presentation] New insight of Engineering Expression System based on ultrafast DNA and RNA photo-cross-linking (2015)
  • Author(s): Kenzo Fujimoto, Shigetaka Nakamura
  • Organizer: PEGS Europe 2015
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2015-11-03
  • Int'l Joint Research
• [Presentation] 核酸医薬を志向した光化学的な核酸類操作法の開発 (2015)
  • Author(s): 藤本健造
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県、名古屋市）
  • Year and Date: 2015-10-08
  • Invited
• [Presentation] Development about 19F chemical shift imaging of DNA conformation change (2015)
  • Author(s): Kenzo Fujimoto, Shigetaka Nakamura
  • Organizer: World molecular Imaging Conference 2015
  • Place of Presentation: Hawaii, U.S.A
  • Year and Date: 2015-09-03
  • Int'l Joint Research
• [Presentation] 光を用いた核酸類操作法の開発 (2015)
  • Author(s): 藤本健造
  • Organizer: バイオアカデミックフォーラム
  • Place of Presentation: 東京ビッグサイト（東京都、江東区）
  • Year and Date: 2015-05-04
• [Presentation] Photochemical regulation of DNA strand exchange using DNA photocrosslinking (2014)
  • Author(s): Shigetaka Nakamura, Hirokazu Hashimoto, Kenzo Fujimoto
  • Organizer: 20th International Conference on DNA Computing and Molecular Programming (DNA20)
  • Place of Presentation: Kyoto Univ., Kyoto, Japan
  • Year and Date: 2014-09-22
• [Presentation] Improvement of thermal stability of DNA structure by photocrosslinking reaction using 3-cyanovinylcarbazole (2014)
  • Author(s): Shigetaka Nakamura, Kenzo Fujimoto
  • Organizer: Challenges in Nanoscience ISACS 15
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-08-18
• [Presentation] Creation of DNA array structure equipped with heat resistance by ultrafast photocrosslinking using 3-cyanovintlcarbazole (2014)
  • Author(s): Kenzo Fujimoto
  • Organizer: XXVth IUPAC ON PHOTOCHEMISTRY
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2014-07-05
• [Book] Chemistry and Applications in Mofified Nucleic Acids (2016)
  • Author(s): Takashi Sakamoto, Kenzo Fujimoto
  • Total Pages: 14
  • Publisher: Springer
• [Book] Mofified Nucleic Acids (2016)
  • Author(s): Takashi Sakamoto; Kenzo Fujimoto
  • Total Pages: 14
  • Publisher: Springer
• [Patent(Industrial Property Rights)] アンチジーン法用光応答性人工核酸プローブ (2016)
  • Inventor(s): 藤本健造、中村重孝
  • Industrial Property Rights Holder: 国立大学法人北陸先端科学技術大学院大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-105622
  • Filing Date: 2016-05-26
• [Patent(Industrial Property Rights)] 含フッ素化合物、それを用いる核酸検出方法 (2015)
  • Inventor(s): 坂本隆, 藤本健造
  • Industrial Property Rights Holder: 国立大学法人 北陸先端科学技術大学院大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-044611
  • Filing Date: 2015-03-06
• [Patent(Industrial Property Rights)] 光架橋核酸二重鎖の光架橋を光開裂させる方法 (2014)
  • Inventor(s): 藤本健造, 中村重孝
  • Industrial Property Rights Holder: 国立大学法人 北陸先端科学技術大学院大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-224574
  • Filing Date: 2014-11-04