Development about photochemical detection of nuceic acids by 19F-NMR
Project/Area Number |
26288077
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
|
Research Institution | Japan Advanced Institute of Science and Technology |
Principal Investigator |
Fujimoto Kenzo 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (90293894)
|
Co-Investigator(Kenkyū-buntansha) |
坂本 隆 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 助教 (80423078)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
Keywords | 19FーNMR / 核酸検出 / ケミカルシフト / 光架橋反応 / 19F-NMRプローブ / 光クロスリンク / 19F / 核酸検出プローブ / 19F NMR / 19F NMR |
Outline of Final Research Achievements |
We demonstrated an approach for the chemical shift-based detection of nucleic acids based on CNVK (cyanovinylcarobazole) mediated DNA/RNA photo-cross-linking. The 19F NMR signal of TFT(trifluoromethyl thymidine) in a DNA duplex structure was dramatically shifted from -63.2 ppm to -71.2 ppm induced by photo-cross-linking. This larger chemical shift change induced by CNVK mediated DNA photo-cross-linking enables DNA and RNA detection without confounding effects due to the influence of neighboring bases. We have also successfully demonstrated that 10 nM miRNA can be detected in a sequence-specific manner based on miRNA amplification and chemical shift caused by the photo-cross-linking of CNVK and TFT in DNA and RNA.
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Report
(4 results)
Research Products
(60 results)