Preparation of biocatalyst-entrapped macroporous gel using a suspension-gelation method

• Project/Area Number: 26289284
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Properties in chemical engineering process/Transfer operation/Unit operation
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Tokuyama Hideaki 東京農工大学, 工学(系)研究科(研究院), 准教授 (10363029)
• Co-Investigator(Kenkyū-buntansha): 寺田 昭彦 東京農工大学, 工学(系)研究科(研究院), 准教授 (30434327)
• Co-Investigator(Renkei-kenkyūsha): NAGATSU Yuichiro 東京農工大学, 大学院工学研究院, 准教授 (60372538)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 化学工学 / 反応・分離工学 / 酵素 / 微生物 / 高分子合成

Outline of Final Research Achievements

We proposed and developed the suspension-gelation method to prepare macroporous gels for immobilization of biocatalyst such as enzyme and bacteria. Using this technique, in situ entrapment of biocatalyst within macropores in the gels is achieved without requiring contact between the reactive chemical species and biocatalyst during polymerization. The performance and feasibility of biocatalyst-entrapped macroporous gel were demonstrated for the biocatalyzed reaction such as biodiesel production and ammonia oxidation.

Research Products

• [Journal Article] Fabrication of enzyme-entrapped composite and macroporous gel beads by suspension gelation combined with sedimentation polymerization (2016)
  • Author(s): Ryuichi Sato, Hideaki Tokuyama
  • Journal Title: Biochemical Engineering Journal Volume: 113 Pages: 152-157
  • DOI: 10.1016/j.bej.2016.06.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preparation of macroporous poly(N-isopropylacrylamide) hydrogels using a suspension-gelation method (2015)
  • Author(s): Ryuichi Sato, Ryoko Noma, Hideaki Tokuyama
  • Journal Title: European Polymer Journal Volume: 66 Pages: 91-97
  • DOI: 10.1016/j.eurpolymj.2015.01.051
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fabrication of amphiphilic copolymeric gels with enhanced activity of immobilized enzymes in organic media (2015)
  • Author(s): Ryuichi Sato, Ayumi Sato, Hideaki Tokuyama
  • Journal Title: Journal of Applied Polymer Science Volume: 132 Issue: 17
  • DOI: 10.1002/app.41905
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Fabrication of lipase-entrapped amphiphilic copolymeric gel beads and their use for transesterification reaction in organic media (2016)
  • Author(s): Ayaka Naito, Hideaki Tokuyama
  • Organizer: 12th Japan-Korea Symposium on Materials & Interfaces
  • Place of Presentation: 御殿場高原時之栖(静岡県御殿場市)
  • Int'l Joint Research
• [Presentation] サスペンションゲル化法を用いた酵素固定化多孔質ゲル粒子の開発および酵素反応特性 (2016)
  • Author(s): 徳山英昭, 佐藤龍一
  • Organizer: 第65回高分子学会年次大会
  • Place of Presentation: 神戸国際会議場・展示場(兵庫県神戸市)
• [Presentation] 酵素固定化高分子ゲルの繰り返し利用性能に関する研究 (2016)
  • Author(s): 内藤絢香, 徳山英昭
  • Organizer: 分離技術会年会2016
  • Place of Presentation: 日本大学津田沼キャンパス(千葉県習志野市)
• [Presentation] Ammonium oxidation reaction catalyzed by bacteria entrapped within macroporous polymeric hydrogels using a suspension-gelation method (2015)
  • Author(s): Ryuichi Sato, Ryoko Noma, Hideaki Tokuyama
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: ホノルル市（アメリカ合衆国）
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] Esterification in organic media using lipase immobilized within amphiphilic NIPA-co-PEGMEA gels (2015)
  • Author(s): Hideaki Tokuyama, Ryuichi Sato, Ayumi Sato
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)
  • Place of Presentation: ホノルル市（アメリカ合衆国）
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] サスペンションゲル化法を用いた酵素固定化多孔質ゲルの開発 (2015)
  • Author(s): 佐藤龍一, 徳山英昭
  • Organizer: 化学工学会第47回秋季大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-09-10
• [Presentation] 酵素固定化両親媒性ゲルの開発と油性媒体中でのエステル化反応の特性 (2015)
  • Author(s): 佐藤龍一, 佐藤あゆみ, 徳山英昭
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-05-27
• [Presentation] サスペンションゲル化法による感温性多孔質semi-IPNゲルの創製 (2015)
  • Author(s): 徳山英昭, 野間涼子, 佐藤龍一
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-05-27
• [Presentation] Preparation of Macroporous Hydrogels for The Immobilization of Bacteria Using A Suspension-gelation Method (2014)
  • Author(s): Ryuichi Sato, Ryoko Noma, Hideaki Tokuyama
  • Organizer: The Materials Research Society of Indonesia (MRS-Id) Meeting 2014
  • Place of Presentation: Denpasar（Indonesia）
  • Year and Date: 2014-09-28
• [Presentation] 油性媒体中で高活性を発現する酵素固定化高分子ゲルの創製 (2014)
  • Author(s): 佐藤あゆみ, 佐藤龍一, 徳山英昭
  • Organizer: 化学工学会第46回秋季大会
  • Place of Presentation: 九州大学（福岡県）
  • Year and Date: 2014-09-18
• [Presentation] サスペンションゲル化法による感温性多孔質ゲルの創製 (2014)
  • Author(s): 野間涼子, 佐藤龍一, 徳山英昭
  • Organizer: 化学工学会第46回秋季大会
  • Place of Presentation: 九州大学（福岡県）
  • Year and Date: 2014-09-18
• [Remarks] 徳山研究室ホームページ
  • URL: http://web.tuat.ac.jp/~tokuyama/
• [Remarks] 徳山研究室ホームページ
  • URL: http://www.tuat.ac.jp/~tokuyama/