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Establishment of KFRS4 rats as a model of atopic dermatitis

Research Project

Project/Area Number 26290031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionKyoto University

Principal Investigator

Kuramoto Takashi  京都大学, 医学(系)研究科(研究院), 准教授 (20311409)

Co-Investigator(Kenkyū-buntansha) 椛島 健治  京都大学, 医学(系)研究科(研究院), 教授 (00362484)
吉見 一人  国立遺伝学研究所, 大学共同利用機関等の部局等, 助教 (50709813)
Co-Investigator(Renkei-kenkyūsha) HIAI Hiroshi  京都大学, 名誉教授 (10073131)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsアトピー性皮膚炎 / ラット / 疾患モデル / IgE / 引掻き行動 / 好酸球 / マスト細胞 / ステロイド / ハプテン / アレルギー・ぜんそく / 免疫学 / 薬理学 / 応用動物 / タクロリムス / 引っかき行動
Outline of Final Research Achievements

Rats showing spontaneous atopic dermatitis (AD)-like skin lesions were observed in the Kyoto fancy rat stock 4 (KFRS4) rats. To establish the KFRS4 rat as a model of AD, clinical symptom, scratching behavior, and transepidermal water loss (TEWL) were examined. Dermatitis initially appeared around 4 months of age and rapidly worsened from 6 to 8 months of age. The skin lesions accompanied scratching behavior and were predominantly observed in females. The increased TEWL indicated skin barrier dysfunction. Extensive infiltration of eosinophils, mast cells and lymphocytes was observed in the skin lesions. The plasma IgE level increased in accord with increasing severity of dermatitis. The betamethasone improved the symptoms of dermatitis. These findings demonstrated that dermatitis in the KFRS4 rats closely resembled that seen in human AD, which suggested that female KFRS4 rats have the potential to serve as an animal model of human AD.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (12 results)

All 2016 2015 2014 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains2016

    • Author(s)
      Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M
    • Journal Title

      Genomic Data

      Volume: 10 Pages: 155-157

    • DOI

      10.1016/j.gdata.2016.11.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Design and application of a target capture sequencing of exons and conserved non-coding sequences for the rat2016

    • Author(s)
      Yoshihara M, Saito D, Sato T, Ohara O, Kuramoto T, Suyama M
    • Journal Title

      BMC Genomics

      Volume: 17 Issue: 1 Pages: 593-593

    • DOI

      10.1186/s12864-016-2975-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A distal 594bp ECR specifies Hmx1 expression in pinna and lateral facial morphogenesis and is regulated by Hox-Pbx-Meis2016

    • Author(s)
      Rosin JM, Li W, Cox LL, Rolfe SM, Latorre V, Akiyama JA, Visel A, Kuramoto T, Bobola N, Turner EE, Cox TC
    • Journal Title

      Development

      Volume: 143 Pages: 2582-2592

    • DOI

      10.1242/dev.133736

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Atopic dermatitis-like skin lesions with IgE hyperproduction and pruritus in KFRS4/Kyo rats2015

    • Author(s)
      Kuramoto T, Yokoe M, Tanaka D, Yuri A, Nishitani A, Higuchi Y, Yoshimi K, Tanaka M, Kuwamura M, Hiai H, Kabashima K, Serikawa T
    • Journal Title

      Journal of Dermatological Sciences

      Volume: 80 Issue: 2 Pages: 116-123

    • DOI

      10.1016/j.jdermsci.2015.09.005

    • NAID

      120005707937

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] アトピー性皮膚炎モデルラットの治療試験2016

    • Author(s)
      庫本高志、横江繭子、西谷あい、日合 弘、椛島健治
    • Organizer
      第63回日本実験動物学会
    • Place of Presentation
      ミューザ川崎シンフォニーホール(神奈川県川崎市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] アトピー性皮膚炎モデルラットの治療試験2016

    • Author(s)
      庫本高志、横江繭子、西谷あい、日合 弘、椛島健治
    • Organizer
      第63回日本実験動物学会
    • Place of Presentation
      ミューザ川崎シンフォニーホール(神奈川県・川崎市)
    • Year and Date
      2016-05-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Atopic dermatitis-like skin lesions with IgE hyperproduction and pruritus in KFRS4/Kyo rats2015

    • Author(s)
      Kuramoto T, Yokoe M, Tanaka D, Yuri A, Nishitani A, Higuchi Y, Yoshimi K, Tanaka M, Kuwamura M, Hiai H, Kabashima K, Serikawa T
    • Organizer
      Rat Genomics & Models
    • Place of Presentation
      Cold Spring Harbor Laboratory(アメリカ合衆国)
    • Year and Date
      2015-12-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アトピー性皮膚炎モデルラットの治療試験2015

    • Author(s)
      庫本高志、横江繭子、西谷あい、由利 梓、日合 弘、椛島健治
    • Organizer
      第128回関西実験動物研究会
    • Place of Presentation
      京都大学楽友会館(京都府・京都市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新たなアトピー性皮膚炎モデルの開発2015

    • Author(s)
      庫本高志、横江繭子、由利 梓、西谷あい、田中大資、日合 弘、芹川忠夫
    • Organizer
      第62回日本実験動物学会
    • Place of Presentation
      京都テルサ(京都府・京都市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新たなアトピー性皮膚炎モデルの開発2014

    • Author(s)
      樋口裕城、横江繭子、由利 梓、田中大資、日合 弘、芹川忠夫、庫本高志
    • Organizer
      第124回関西実験動物研究会
    • Place of Presentation
      京都大学楽友会館
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Remarks] 新たなアトピー性皮膚炎モデルラットを開発

    • URL

      http://www.anim.med.kyoto-u.ac.jp/Kuramoto/contents/KFRS4Atopy.htm

    • Related Report
      2016 Annual Research Report
  • [Remarks] 庫本高志のホームページ

    • URL

      http://www.anim.med.kyoto-u.ac.jp/Kuramoto/home.htm

    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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