Elucidating the mechanisms of colorectal cancer metastasis using mouse models
| Project/Area Number |
26290045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
Aoki Masahiro 愛知県がんセンター(研究所), 分子病態学部, 部長 (60362464)
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| Co-Investigator(Kenkyū-buntansha) |
梶野 リエ 愛知県がんセンター(研究所), 分子病態学部｀, 研究員 (20633184)
小島 康 愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (30464217)
藤下 晃章 愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (50511870)
佐久間 圭一朗 愛知県がんセンター(研究所), 分子病態学部, 室長 (90402891)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEDA Junji 大阪大学, 医学研究科, 教授 (50163407)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 大腸がん / 転移 / マウスモデル / スプライシング / RNA結合タンパク / トランスポゾン / 遺伝子改変マウス / 動物実験 / 上皮間葉転換 |
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| Outline of Final Research Achievements |
We identified HNRNPLL, an RNA binding protein, as a novel suppressor of colorectal cancer metastasis through a functional screen in mice. Reduced expression of HNRNPLL in colorectal cancer cells conferred increased their invasion ability in vitro and metastatic ability in vivo. HNRNPLL was shown to suppress invasion of colorectal cancer cells at least partly via controlling the alternative splicing of CD44 pre-mRNA. HNRNPLL expression was downregulated during epithelial-mesenchymal transition (EMT) of colorectal cancer cells, and immunohistochemical analysis of colorectal cancer clinical samples further suggested the link between the HNRNPLL level and EMT. HNRNPLL was also shown to stabilize mRNAs encoding the DNA replication factors, and to enhance proliferation of colorectal cancer cells.
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Report
(5 results)
Research Products
(43 results)
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[Journal Article] Global metabolic reprogramming of colorectal cancer occurs at adenoma stage and is induced by MYC2017
Author(s)
Satoh K, Yachida S, Sugimoto M, Oshima M, Nakagawa T, Akamoto S, Tabata S, Saitoh K, Kato K, Sato S, Igarashi K, Aizawa Y, Kajino-Sakamoto R, Kojima Y, Fujishita T, Enomoto A, Hirayama A, Ishikawa T, Taketo MM, Kushida Y, Haba R, Okano K, Tomita M, Suzuki Y, Fukuda S, Aoki M, Soga T.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 114
Issue: 37
DOI
Related Report
Peer Reviewed
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[Journal Article] Association between and polymorphisms and adverse drug reactions to regorafenib: A preliminary study2017
Author(s)
Maeda A, Ando H, Ura T, Komori A, Hasegawa A, Taniguchi H, Kadowaki S, Muro K, Tajika M, Kobara M, Matsuzaki M, Hashimoto N, Maeda M, Kojima Y, Aoki M, Kondo E, Mizutani A, Fujimura A.
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Journal Title
Int. J. Clin. Pharmacol. Ther
Volume: epub ahead of print
Issue: 05
Pages: 409-415
DOI
Related Report
Peer Reviewed
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[Journal Article] Genomic loss of DUSP4 contributes to the progression of intraepithelial neoplasm of pancreas to invasive carcinoma.2016
Author(s)
Hijiya N, Tsukamoto Y, Nakada C, Tung NL, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.
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Journal Title
Cancer Res.
Volume: 76
Issue: 9
Pages: 2612-2625
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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