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Analysis of the imprinted regions based on the entire transcriptome

Research Project

Project/Area Number 26290063
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Genome biology
Research InstitutionKochi University

Principal Investigator

KIYOSAWA Hidenori  高知大学, 医学部, 特任准教授 (30295422)

Co-Investigator(Kenkyū-buntansha) 近藤 伸二  大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, 准教授 (30415161)
加藤 英政  埼玉医科大学, 医学部, 准教授 (50292123)
栄徳 勝光  高知大学, 教育研究部医療学系連携医学部門, 助教 (50552733)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsゲノム刷り込み / トランスクリプトーム / エピジェネティクス / 幹細胞 / 神経分化 / ゲノム / エピゲノム
Outline of Final Research Achievements

We attempted to identify novel neuron-specific imprinted region with the samples utilizing in vitro neuronal differentiation system and RNA-seq. We performed transcriptome analysis with those data, and identified novel imprinted regions, including the regions producing non-coding RNAs. We also performed a similar analysis with iPS cells, and identified difference from the ES cell data. We obtained the ChIP-seq data with H3K36 and TSS-seq data, and analyzed the transcriptional landscape of long non-coding RNAs.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Comparison of two types of non-adherent plate for neuronal differentiation of mouse embryonic stem cells2016

    • Author(s)
      Eitoku M, Suganuma N, Kiyosawa H
    • Journal Title

      Cytotechnology

      Volume: - Issue: 6 Pages: 2761-2768

    • DOI

      10.1007/s10616-016-9968-z

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] デフォルト神経分化過程での亜種特異的モノアレル発現2016

    • Author(s)
      栄徳勝光、近藤伸二、鈴木穣、高田豊行、加藤英政、城石俊彦、菅沼成文、清澤秀孔
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Subspecies-specific monoallelic expression during neural differentiation2016

    • Author(s)
      Eitoku M, Kondo S, Suzuki Y, Takada T, Kato H, Shiroishi T, Suganuma N, Kiyosawa H
    • Organizer
      The 21st Annual Meeting of The RNA Society
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] デフォルト神経分化による神経特異的ゲノム刷り込み領域における縦断的エピジェネティック解析2015

    • Author(s)
      栄徳勝光、近藤伸二、鈴木穣、高田豊行、加藤英政、城石俊彦、菅沼成文、清澤秀孔
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド 兵庫県
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 10週間に亘るマウス胚幹(ES)細胞の神経分化過程におけるF1ハイブリッドマウス対4アレルのゲノム上のポリA鎖付加を伴う且つ伴わない転写活動の動的変化の比較解析2015

    • Author(s)
      近藤伸二、加藤英政、鈴木穣、高田豊行、城石俊彦、菅沼成文、清澤秀孔
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド 兵庫県
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] in vitro神経細胞分化系を用いた網羅的な組織特異的ゲノム刷り込み領域の解析2014

    • Author(s)
      近藤伸二、加藤英政、鈴木穣、高田豊行、城石俊彦、菅沼成文、清澤秀孔
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 亜種間雑種ES細胞を用いた組織特異的ゲノム刷り込み解析2014

    • Author(s)
      近藤伸二、加藤英政、鈴木穣、高田豊行、城石俊彦、菅沼成文、清澤秀孔
    • Organizer
      第16回日本RNA学会年会
    • Place of Presentation
      ウィンクあいち(愛知県)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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