Structural basis for membrane protein integration
Project/Area Number |
26291023
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Tsukazaki Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
|
Co-Investigator(Renkei-kenkyūsha) |
TANAKA Yoshiki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10632333)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 蛋白質 / Sec / 膜タンパク質 / タンパク輸送 / SecYEG / X線結晶構造解析 / 膜組込み / 構造生命科学 / タンパク質輸送 / X線結晶構造解析 / 蛋白質の膜組込み / YidC / トランスロコン / 膜蛋白質 / 組込み / 蛋白質輸送 |
Outline of Final Research Achievements |
Crystal structures of full-length and peptide-bound SecYEG were determined. The cytoplasmic loop of SecG covers the protein-conducting channel. The cytoplasmic loop of SecG blocks protein translocation. Crystal structures of SecDF in I forms are described. The structures provide insight into an energy-coupling mechanism for Sec machinery. The structures suggest a substrate binding site and a pathway for protons.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Tunnel formation inferred from the I form structures of the proton-driven protein secretion motor SecDF2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
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Journal Title
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein.2015
Author(s)
Shimokawa-Chiba, N., Kumazaki, K., Tsukazaki, T., Nureki, O., Ito, K. and Chiba, S.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 112
Issue: 16
Pages: 5063-5068
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] 7.Crystal Structures of SecYEG in Lipidic Cubic Phase Elucidate a Precise Resting and a Peptide-Bound State2015
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki, Tomoya Tsukazaki
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Journal Title
Cell report
Volume: 13
Issue: 8
Pages: 1561-1568
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Structural basis for Sec-independent membrane protein insertion by YidC.2014
Author(s)
Kumazaki, K., Chiba, S., Takemoto, M., Furukawa, A., Nishiyama, K., Sugano, Y., Mori, T., Dohmae, N., Hirata, K., Nakada-Nakura, Y., Maturana, A.D., Tanaka, Y., Mori, H., Sugita, Y., Arisaka, F., Ito K., Ishitani, R., Tsukazaki, T. and Nureki, O.
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Journal Title
Nature
Volume: 509
Issue: 7501
Pages: 516-520
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans.2014
Author(s)
Kumazaki, K., Tsukazaki, T., Nishizawa, T., Tanaka, Y., Kato, H.E., Nakada-Nakura, Y., Hirata, K., Mori, Y., Suga, H., Dohmae, N., Ishitani, R. and Nureki, O.
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Journal Title
Acta Crystallographica Section F Structural Biology Communications
Volume: 70
Issue: 8
Pages: 1056-1060
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase2014
Author(s)
Kumazaki, K., Kishimoto, T., Furukawa, A., Mori, H., Tanaka, Y., Dohmae, N., Ishitani, R., Tsukazaki, T., and Nureki, O.
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Journal Title
Scientific reports
Volume: 4
Issue: 1
Pages: 7299-7299
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 膜輸送装置の構造と機能2017
Author(s)
田中 良樹, 古川 新, 森 貴治, 森 博幸, 森本 雄祐, 菅野 泰功, 岩木 薫大, 南野 徹, 杉田 有治, 塚﨑 智也
Organizer
第90回 日本細菌学会総会
Place of Presentation
仙台国際センター(宮城県・仙台市)
Year and Date
2017-03-19
Related Report
Invited
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[Presentation] Snapshots of the proton-driven protein translocation motor2017
Author(s)
Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
Organizer
EMBO conference | Protein translocation and cellular homeostasis
Place of Presentation
Hotel Valamar Lacroma (クロアチア・ドブロブニク)
Year and Date
2017-03-18
Related Report
Int'l Joint Research / Invited
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[Presentation] 大腸菌のペリプラズムプロテアーゼBepAは、TPR domainを介した タンパク質間相互作用により外膜機能を維持する2017
Author(s)
大門 康志, 舛井 千草, 宮﨑 亮次, 櫻田 洋人, 田中 良樹, 鈴木 健裕, 堂前 直, 森 博幸, 塚崎 智也, 成田 新一郎, 秋山 芳展
Organizer
日本農芸化学会2017年度大会
Place of Presentation
ウェスティン都ホテル(京都府・京都市)
Year and Date
2017-03-17
Related Report
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[Presentation] タンパク質膜透過を駆動するモータータンパク質のスナップショット2017
Author(s)
古川 新, 吉海江 国仁, 森 貴治, 森 博幸, 森本 雄祐, 菅野 泰功, 岩木 薫大, 南野 徹, 杉田 有治, 田中 良樹, 塚崎 智也
Organizer
2017年 生体運動研究合同班会議
Place of Presentation
神戸国際会議場(兵庫県・神戸市)
Year and Date
2017-01-06
Related Report
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[Presentation] Snapshots of a protein translocation motor2016
Author(s)
古川 新, 吉海江 国仁, 森 貴治, 森 博幸, 森本 雄祐, 菅野 泰功, 岩木 薫大, 南野 徹, 杉田 有治, 田中 良樹, 塚崎 智也
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県・つくば市)
Year and Date
2016-11-25
Related Report
Int'l Joint Research / Invited
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[Presentation] Snapshots of a proton-driven protein translocation motor2016
Author(s)
Tomoya Tsukazaki, Kunihito Yoshikaie, Arata Furukawa, Yasunori Sugano, Yoshiki Tanaka
Organizer
The 42nd Naito Conference on In the Vanguard of Structural Biology: Revolutionizing Life Sciences
Place of Presentation
シャトレーゼ ガトーキングダム サッポロ(北海道・札幌市)
Year and Date
2016-10-04
Related Report
Int'l Joint Research / Invited
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[Presentation] Complete and Peptide-bound Structures of the Sec translocon2016
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki, Tomoya Tsukazaki
Organizer
Nascent Chain Biology Meeting 2016
Place of Presentation
富士レークホテル(山梨県・富士河口湖町)
Year and Date
2016-09-01
Related Report
Int'l Joint Research
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[Presentation] 新生鎖を膜透過させるSec蛋白質のX線結晶構造解析2016
Author(s)
田中 良樹, 菅野 泰功, 武本 瑞貴, 森 貴治, 古川 新, 吉海江 国仁, 草木迫 司, 熊崎 薫, 鹿島 絢子, 石谷 隆一郎, 杉田 有治, 濡木 理, 塚崎 智也
Organizer
第16回日本蛋白質科学会 年会
Place of Presentation
福岡国際会議場(福岡県・福岡市)
Year and Date
2016-06-06
Related Report
Invited
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[Presentation] Complete and peptide-bound structures of the Sec translocon2015
Author(s)
Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki, Tomoya Tsukazaki
Organizer
新学術領域研究「動的秩序と機能」第4回公開国際シンポジウム
Place of Presentation
九州大学(福岡県・福岡市)
Year and Date
2015-11-22
Related Report
Int'l Joint Research
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[Presentation] Structures of Membrane Protein Insertase YidC2015
Author(s)
Tomoya Tsukazaki, Kaoru Kumazaki, Shinobu Chiba, Mizuki Takemoto, Arata Furukawa, Toshiki Kishimoto, Yasunori Sugano, Takaharu Mori, Yoshiki Tanaka, Yuji Sugita, Koreaki Ito, Ryuichiro Ishitani, Osamu Nureki
Organizer
Structural Biological Dynamics: From Molecules to Life with 60 trillion Cells
Place of Presentation
東京大学(東京都・文京区)
Year and Date
2015-11-05
Related Report
Int'l Joint Research
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[Presentation] Structure and dynamics of Sec protein-conducting channel2015
Author(s)
Yasunori Sugano, Yoshiki Tanaka, Mizuki Takemoto, Takaharu Mori, Takamitsu Haruyama, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Hiroki Konno, Yuji Sugita, Osamu Nureki, Tomoya Tsukazaki
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県・金沢市)
Year and Date
2015-09-13
Related Report
Int'l Joint Research
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[Presentation] YidCによるタンパク質膜組込機構の解明2014
Author(s)
千葉志信,熊崎薫,塚崎智也,濡木理,伊藤維昭
Organizer
第37回 日本分子生物学会年会
Place of Presentation
パシフィコ横浜(神奈川県・横浜市)
Year and Date
2014-11-25 – 2014-11-27
Related Report
Invited
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