Role of regions responsible for the interaction between subunits of a voltage-gated calcium channel of non-excitable cells

• Project/Area Number: 26291026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo Gakugei University
• Principal Investigator: IIDA Hidetoshi 東京学芸大学, 教育学部, 名誉教授 (70124435)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 電位作動性カルシウムチャネル / サブユニット / タンパク質間相互作用 / 出芽酵母 / 電位依存性カルシウムチャネル / 電位作動性Ca2+チャネル / 電位依存性Ca2+チャネル / シグナル伝達

Outline of Final Research Achievements

We have studied the structure and function of Cch1 and Mid1, two major subunits of a voltage-gated calcium channel homologue of non-excitable yeast cells. We have determined amino acid residues in each subunit, which are essential for the interaction between the two subunits. In addition, we have found that N-terminal 209 amino acid residues of Mid1 are not required for the function of Mid1, which is composed of 548 amino acid residues, although Mid1 has the N-terminal signal peptide composed of 20 amino acid residues.

Research Products

• [Journal Article] Ca2+-permeable mechanosensitive channels MCA1 and MCA2 mediate cold-induced cytosolic Ca2+ increase and cold tolerance in Arabidopsis (2018)
  • Author(s): Mori Kendo、Renhu Na、Naito Maho、Nakamura Aki、Shiba Hayato、Yamamoto Tsuyoshi、Suzaki Takuya、Iida Hidetoshi、Miura Kenji
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 550-550
  • DOI: 10.1038/s41598-017-17483-y
  • NAID: 120007134505
  • Peer Reviewed / Open Access
• [Journal Article] Post-translational processing and membrane translocation of the yeast regulatory Mid1 subunit of the Cch1/VGCC/NALCN cation channel family (2017)
  • Author(s): Iida Kazuko、Teng Jinfeng、Cho Toshihiko、Yoshikawa-Kimura Sato、Iida Hidetoshi
  • Journal Title: Journal of Biological Chemistry Volume: 292 Issue: 50 Pages: 20570-20582
  • DOI: 10.1074/jbc.m117.810283
  • Peer Reviewed
• [Journal Article] Sensors Make Sense of Signaling (2017)
  • Author(s): A.R. Webb Alex、Kuchitsu Kazuyuki、Kwak June、Pei Zhen-Ming、Iida Hidetoshi
  • Journal Title: Plant and Cell Physiology Volume: 58 Issue: 7 Pages: 1121-1125
  • DOI: 10.1093/pcp/pcx085
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic analysis of the regulation of the voltage-gated calcium channel homolog Cch1 by the γ subunit homolog Ecm7 and cortical ER protein Scs2 in yeast (2017)
  • Author(s): Kato Takafumi、Kubo Aya、Nagayama Tatsuya、Kume Shinichiro、Tanaka Chikara、Nakayama Yoshitaka、Iida Kazuko、Iida Hidetoshi
  • Journal Title: PLOS ONE Volume: 12 Issue: 7 Pages: 1-17
  • DOI: 10.1371/journal.pone.0181436
  • Peer Reviewed / Open Access
• [Journal Article] Coupling of a voltage-gated Ca2+ channel homologue with a plasma membrane H+-ATPase in yeast (2017)
  • Author(s): Cho T, Ishii-Kato A, Fukata Y, Nakayama Y, Iida K, Fukata M, Iida H
  • Journal Title: Genes Cells Volume: 22 Issue: 1 Pages: 94-104
  • DOI: 10.1111/gtc.12458
  • Peer Reviewed / Open Access
• [Journal Article] Transmembrane topologies of Ca^2+-permeable mechanosensitive channels MCA1 and MCA2 in <i>Arabidopsis thaliana</i>. (2015)
  • Author(s): Kamano, S., Kume, S., Iida, K., Lei, K.-J., Nakano, M., Nakayama, Y., and Iida, H.
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 52 Pages: 30901-9
  • DOI: 10.1074/jbc.m115.692574
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of Ca2+ in vacuole degradation caused by a rapid temperature decrease in Saintpaulia palisade cells: A case of gene expression analysis in a specialized small tissue. (2015)
  • Author(s): Ohnishi M., Kadohama N., Suzuki Y., Kajiyama T., Shichijo C., Ishizaki K., Fukaki H., Iida H., Kambara H., Mimura T.
  • Journal Title: Plant & Cell Physiology Volume: 印刷中 Issue: 7 Pages: 1297-1035
  • DOI: 10.1093/pcp/pcv048
  • Peer Reviewed / Open Access
• [Journal Article] Organellar mechanosensitive channels involved in hypo-osmoregulation in fission yeast. (2014)
  • Author(s): Nakayama, Y., and Iida, H.
  • Journal Title: Cell Calcium, Volume: 56 Pages: 467-471
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mechanosensitive channels Msy1 and Msy2 are required for maintaining organelle integrity upon hypoosmotic shock in Schizosaccharomyces pombe. (2014)
  • Author(s): Nakayama, Y., Hirata, A., and Iida, H.
  • Journal Title: FEMS Yeast Res. Volume: 14 Issue: 6 Pages: 992-994
  • DOI: 10.1111/1567-1364.12181
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Molecular interaction between two core subunits of a yeast calcium channel related to mammalian VGCCs and NALCNs (2018)
  • Author(s): Hidetoshi Iida, Takuto Hayashi, Keita Oishi, Midori Kimura, and Kazuko Iida
  • Organizer: 3rd European Calcium Channel Conference
  • Int'l Joint Research
• [Presentation] シロイヌナズナの精製機械受容チャネルタンパク質のイオン透過性 (2018)
  • Author(s): 飯田秀利、飯田和子、池邊大輝、吉村建二郎
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] セントポーリアの温度降下感受性に関わる分子機構の解析 (2018)
  • Author(s): 本岡香奈、大西美輪、飯田和子、角浜憲明、鈴木祥弘、石崎公庸、深城英弘、飯田秀利、三村徹郎
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 根の貫入が不可能な硬さの培地の表面で生き残れるシロイヌナズナ突然変異株のスクリーニング (2017)
  • Author(s): 東條宏史、中村あき、アリ・フェルジャニ、風間裕介、阿部知子、飯田秀利
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県・鹿児島市）
  • Year and Date: 2017-03-18
• [Presentation] Mechanosensing in plants (2016)
  • Author(s): Iida, H.
  • Organizer: The 3rd Annual Meeting of the Society for Bioacoustics
  • Place of Presentation: Irago Sea-Park & Spa (Irago-cho, Tahara-shi, Aichi, Japan)
  • Year and Date: 2016-12-10
  • Int'l Joint Research / Invited
• [Presentation] 電位作動性Ca2+チャネルα1サブユニットの出芽酵母ホモログCch1のドメインⅢ内変異の網羅的解析 (2016)
  • Author(s): 木村 緑、飯田和子、飯田秀利
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-12-01
• [Presentation] Mechanosensitive channels generating Ca²⁺ signals. (2016)
  • Author(s): IIda, H.
  • Organizer: PCP-sponsored symposium on ROS, Ca²⁺and Plant Sensory Systems 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（盛岡市）
  • Year and Date: 2016-03-19
  • Invited
• [Presentation] 出芽酵母のCa²⁺チャネルサブユニットMid1のN末端配列とシグナル配列非依存的な小胞体内腔への輸送 (2015)
  • Author(s): 長 敏彦、飯田和子、飯田秀利
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（神戸市）
  • Year and Date: 2015-12-03
• [Presentation] Identification of proteins that potentially interact with Ca²⁺-permeable mechanosensitive channels MCA1 and MCA2 in Arabidopsis (2015)
  • Author(s): Nakamura, A. and Iida, H.
  • Organizer: 8th Plant Biomechanics International Conference
  • Place of Presentation: 名古屋大学（名古屋市）
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] Molecular aspects of mechanosensitive channels in plants (2015)
  • Author(s): Iida, H.
  • Organizer: 8th Plant Biomechanics International Conference
  • Place of Presentation: 名古屋大学（名古屋市）
  • Year and Date: 2015-11-30
  • Int'l Joint Research / Invited
• [Presentation] 新規の抗真菌剤開発につながる出芽酵母のMid1/Cch1 Ca²⁺チャネルの基礎研究 (2015)
  • Author(s): 飯田秀利
  • Organizer: 第189回酵母細胞研究会例会
  • Place of Presentation: 東京海洋大学（東京都）
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] シグナルペプチド非依存的な小胞体へのタンパク質輸送 (2015)
  • Author(s): 細見 昭、飯田和子、飯田秀利、鈴木 匡
  • Organizer: 酵母遺伝学フォーラム第48回研究報告会
  • Place of Presentation: 広島大学（東広島市）
  • Year and Date: 2015-08-31
• [Presentation] Ca²⁺チャネルサブユニットMid1のシグナル配列非依存的な小胞体内腔への輸送 (2015)
  • Author(s): 長 敏彦、飯田和子、飯田秀利
  • Organizer: 酵母遺伝学フォーラム第48回研究報告会
  • Place of Presentation: 広島大学（東広島市）
  • Year and Date: 2015-08-31
• [Presentation] 出芽酵母の電位作動性Ca2チャネルのα1サブユニットホモログCch1の小胞体膜タンパク質Scs2による制御 (2014)
  • Author(s): 永山 達也、粂 慎一郎、田中 力、中山 義敬、久保 彩、飯田 和子、飯田 秀利
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] 出芽酵母の電位作動性Ca2+チャネルホモログの活性にはα2/δサブユニットMid1と細胞膜H+-ATPase Pma1との相互作用が大切である (2014)
  • Author(s): 長 敏彦、加藤 彩、深田 優子、久保 彩、中山 義敬、飯田 和子、深田 正紀、飯田 秀利
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] 電位作動性Ca2+チャネルの出芽酵母ホモログのサブユニット間相互作用における細胞外システイン残基の役割 (2014)
  • Author(s): 林 卓人、大石 恵太、新免 実咲、飯田 和子、飯田 秀利
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] 出芽酵母のEcm7は電位作動性Ca2+チャネルホモログの機能を正に制御する (2014)
  • Author(s): 加藤 孝郁、久保 彩、飯田 和子、飯田 秀利
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市）
  • Year and Date: 2014-11-27
• [Presentation] Localization and roles of intracellular MscS-like mechanosensitive channels of protist and yeast. (2014)
  • Author(s): Yoshimura, K., Nakayama, Y. and Iida, H.
  • Organizer: International Symposium on Mechanobiology 2014
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2014-05-22
  • Invited
• [Remarks] 東京学芸大学生命科学分野飯田秀利研究室
  • URL: http://www.u-gakugei.ac.jp/~iida/index.html
• [Remarks] 飯田秀利研究室ホームページ
  • URL: http://www.u-gakugei.ac.jp/~iida/