Mitochondrial dynamics in mammals and its molecular mechanism

• Project/Area Number: 26291032
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Kyushu University
• Principal Investigator: Koshiba Takumi 九州大学, 理学(系)研究科(研究院), 准教授 (70403970)
• Co-Investigator(Kenkyū-buntansha): 渡邊 利雄 奈良女子大学, 自然科学系, 教授 (60201208)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: ミトコンドリア / 分子機構 / 抗ウイルス自然免疫 / 形態調節 / 酵素機能 / 細胞内シグナル伝達 / ダイナミクス

Outline of Final Research Achievements

Mitochondria are dynamic organelles that undergo cycles of homotypic fusion and fission events, which are believed to play an important role in biologic process. Although the importance of mitochondrial dynamics is well documented, the molecular mechanism remain unclear.
Our aims of the project is to elucidate their knowledges of mitochondrial dynamics, and try to understand the mechanism at molecular level. In this project, (1) we generated mice deficient in molecules involved in mitochondrial dynamics, and also (2) we investigated relationship between mitochondrial dynamics and antiviral innate immunity in vitro.

Research Products

• [Journal Article] ウイルスタンパク質とミトコンドリアとの相互作用 (2016)
  • Author(s): 吉住拓馬、安川開、小柴琢己
  • Journal Title: 福岡医学雑誌 Volume: 107 Pages: 148-154
  • NAID: 120005893255
  • Peer Reviewed
• [Journal Article] ミトコンドリア・ダイナミクスと抗ウイルス自然免疫 (2016)
  • Author(s): 小柴琢己
  • Journal Title: 細胞 Volume: 48 Pages: 39-41
  • Acknowledgement Compliant
• [Journal Article] The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion (2016)
  • Author(s): Moriyama, M. et al.
  • Journal Title: J. Virol. Volume: 90 Issue: 8 Pages: 4105-4114
  • DOI: 10.1128/jvi.00120-16
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer (2015)
  • Author(s): Koshiba, T.
  • Journal Title: Biophysics and Physicobiology Volume: 12 Pages: 31-35
  • NAID: 130005098799
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A型インフルエンザウイルスタンパク質PB1-F2とミトコンドリアとの相互作用 (2015)
  • Author(s): 吉住拓馬、小柴琢己
  • Journal Title: 生化学 Volume: 87 Pages: 144-148
  • NAID: 40020383817
  • Peer Reviewed
• [Journal Article] 抗ウイルス自然免疫におけるミトコンドリアの役割 (2015)
  • Author(s): 小柴琢己
  • Journal Title: 細胞工学 Volume: 34 Pages: 571-575
• [Journal Article] Influenza A virus protein PB1-F2 translocates into mitochondria via Tom40 channels and impairs innate immunity (2014)
  • Author(s): Yoshizumi, T., Ichinohe, T., Sasaki, O., Otera, H., Kawabata, S., Mihara, K., and #Koshiba, T.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4713-4713
  • DOI: 10.1038/ncomms5713
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loss of Miro1-directed retrograde mitochondrial movement results in a novel murine model for neuron disease (2014)
  • Author(s): Nguyen, T.T., Oh, S.S., Weaver, D., Lewandowska, A., Maxfield, D., Schuler, M.H., Smith, N.K., Macfarlane, J., Saunders, G., Palmer, C.A., Debattisti, V., Koshiba, T., Pulst, S., Feldman, E.L., Hajnóczky, G., and Shaw, J.M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 111 Issue: 35
  • DOI: 10.1073/pnas.1402449111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The N-terminal Arg residue is essential for autocatalytic activation of a lipopolysaccharide-responsive protease zymogen (2014)
  • Author(s): Kobayashi, Y., Shiga, T., Shibata, T., Sako, M., Maenaka, K., Koshiba, T., Mizumura, H., Oda, T., and Kawabata, S.
  • Journal Title: Journal of Biological Chemistry Volume: 289 Issue: 37 Pages: 25987-25995
  • DOI: 10.1074/jbc.m114.586933
  • Peer Reviewed
• [Presentation] Mitochondrial-mediated antiviral immunity and oxidative phosphorylation (2016)
  • Author(s): 小柴琢己
  • Organizer: 第54回 日本生物物理学会年
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
  • Invited
• [Presentation] Mitochondrial-mediated antiviral innate immunity and oxidative phosphorylation (2016)
  • Author(s): Takumi Koshiba
  • Organizer: The 13th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: Tokyo
  • Year and Date: 2016-10-30
  • Int'l Joint Research / Invited
• [Presentation] Mitochondrial respiration and MAVS-mediated antiviral innate immunity (2016)
  • Author(s): Takumi Koshiba
  • Organizer: International Symposium on "Molecular Basis of Virus-Host Interactions"
  • Place of Presentation: Sapporo
  • Year and Date: 2016-10-22
  • Int'l Joint Research / Invited
• [Presentation] Mitochondria and antiviral signaling (2015)
  • Author(s): 小柴琢己
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Influenza A viral protein PB1-F2 and mitochondria (2015)
  • Author(s): Takumi Koshiba
  • Organizer: The 5th Annual World Congress of Molecular & Cell Biology (CMCB-2015)
  • Place of Presentation: Nanjing, China
  • Year and Date: 2015-04-25
  • Int'l Joint Research / Invited
• [Presentation] 哺乳動物における抗ウイルス免疫とミトコンドリアとの関係 (2014)
  • Author(s): 小柴琢己
  • Organizer: 第25回 フォーラム・イン・ドージン
  • Place of Presentation: 熊本ホテルキャッスル
  • Year and Date: 2014-11-14
  • Invited
• [Presentation] ミトコンドリアと抗RNAウイルス自然免疫 (2014)
  • Author(s): 小柴琢己
  • Organizer: 第52回 日本生物物理学会年
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Remarks] 九州大学研究者情報
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K002718/
• [Remarks] 九州大学理学部生物学科
  • URL: http://www.sci.kyushu-u.ac.jp/