Comprehensive analysis of nitrogen transport from fixed N in nodules and absorbed N in roots in soybean related to nitrate inhibition on nitrogen fixation.

• Project/Area Number: 26292036
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Niigata University
• Principal Investigator: Ohyama Takuji 新潟大学, 自然科学系, 教授 (30152268)
• Co-Investigator(Kenkyū-buntansha): 末吉 邦 新潟大学, 自然科学系, 教授 (10216278) ; 大竹 憲邦 新潟大学, 自然科学系, 准教授 (50313507)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
• Keywords: 植物栄養学 / ダイズ / 窒素固定 / 根粒 / 根 / 硝酸 / アンモニウム / RNAseq

Outline of Final Research Achievements

Concentration of metabolites and RNAseq analysis in gene expression were carried out after the addition of nitrate or ammonium to the nodulated soybean plants. After addition of nitrate and ammonium, the concentration of fructose and glucose were decreased, and organic acid concentration was decreased in the root supplied with ammonium. By the RNAseq analysis of roots and nodules after 8 hours of nitrate treatment, about 12,939 genes were promoted in the roots and 2,431 genes in nodules. The common genes are only 74. The gene expressions were depressed by nitrate treatment, about 8,678 genes in roots, and 1,654 genes in nodules, with common genes 42.

Research Products

• [Journal Article] Estimation of nitrogen fixation rate of soybean (Glycine max (L.) Merr.) by micro-scale relative ureide analysis using root bleeding xylem sap and apoplast fluid in stem. (2014)
  • Author(s): Sakazume, T., Tanaka, K., Aida, H., Ishiwaka, S, Nagumo, Y., Takahashi, Y., Ohtake, N., Sueyoshi, K., Ohyama, T.
  • Journal Title: Bull. Facul. Agric. Niigata University Volume: 67 Pages: 27-41
  • NAID: 120006748756
  • Open Access
• [Presentation] 根粒着生ダイズにおける尿素の存在とウレイド代謝との関係 (2016)
  • Author(s): 小野雄基・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学（仙台市）
  • Year and Date: 2016-09-07
• [Presentation] Nitrate supply enhances the N and C metabolism in the roots but not in the nodules of soybean plants. (2015)
  • Author(s): Shinji ISHIKAWA, Akinori SAITO, Shiori WATANABE, Keisuke ISHIKAWA, Norikuni OHTAKE, Kuni SUEYOSHI, Takuji OHYAMA
  • Organizer: 19th International Congress on Nitrogen Fixation
  • Place of Presentation: 米国、カリフォルニア州
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] 無機態窒素供給によるダイズの根と根粒の代謝成分 (2015)
  • Author(s): 小野雄基・後藤大輝・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-09-14
• [Presentation] 短時間の無機態窒素供給によるダイズ体内成分の変動 (2015)
  • Author(s): 小野雄基・後藤大輝・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 京都大学
  • Year and Date: 2015-09-09
• [Presentation] Effect of Temperature and Light/Dark Conditions on Nodule Growth of soybean (2015)
  • Author(s): Yuki Ono, Daiki Goto, Norikuni Ohtake, Kuni Sueyoshi, Takuji Ohyama
  • Organizer: The 5th Asian Conference on Green Technology in Agriculture: Chances and Challenges for a Better Life
  • Place of Presentation: タイ王国、チェンマイ大学
  • Year and Date: 2015-07-19
• [Presentation] 水耕ダイズに対する無機態窒素供給が根粒肥大と培地pHに与える影響 (2014)
  • Author(s): 小野雄基・後藤大輝・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 日本土壌肥料学会 関東支部大会
  • Place of Presentation: 山梨大学
  • Year and Date: 2014-12-06 – 2014-12-07
• [Presentation] 硝酸添加によるダイズの根粒と根の代謝変動のトランスクリプトーム・メタボローム解析 (2014)
  • Author(s): 石川伸二・石川慶祐・會田浩基・大竹憲邦・末吉邦・大山卓爾
  • Organizer: 植物微生物研究会
  • Place of Presentation: 佐賀大学農学部
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] ダイズ根粒の生長におよぼす温度と日周期の影響 (2014)
  • Author(s): 小野雄基・石川慶祐・石川伸二・大竹憲邦・末吉邦・七夕小百合・七夕高也・大山卓爾
  • Organizer: 植物微生物研究会
  • Place of Presentation: 佐賀大学農学部
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] ポジトロンイメージングによるダイズ植物における窒素固定および固定窒素の輸送の解析 (2014)
  • Author(s): 石井里美・V.P.Hung Ngyuen・鈴井伸郎・尹 永根・河地有木・大山卓爾・藤巻秀
  • Organizer: 日本土壌肥料学会東京大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2014-09-09 – 2014-09-11