| Project/Area Number |
26292036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
|
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| Research Institution | Niigata University |
|---|
Principal Investigator |
Ohyama Takuji 新潟大学, 自然科学系, 教授 (30152268)
|
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| Co-Investigator(Kenkyū-buntansha) |
末吉 邦 新潟大学, 自然科学系, 教授 (10216278)
大竹 憲邦 新潟大学, 自然科学系, 准教授 (50313507)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
|
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| Keywords | 植物栄養学 / ダイズ / 窒素固定 / 根粒 / 根 / 硝酸 / アンモニウム / RNAseq |
|---|
| Outline of Final Research Achievements |
Concentration of metabolites and RNAseq analysis in gene expression were carried out after the addition of nitrate or ammonium to the nodulated soybean plants. After addition of nitrate and ammonium, the concentration of fructose and glucose were decreased, and organic acid concentration was decreased in the root supplied with ammonium. By the RNAseq analysis of roots and nodules after 8 hours of nitrate treatment, about 12,939 genes were promoted in the roots and 2,431 genes in nodules. The common genes are only 74. The gene expressions were depressed by nitrate treatment, about 8,678 genes in roots, and 1,654 genes in nodules, with common genes 42.
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