Development of the methods that inhibits staphylococcal biofilm formation.
| Project/Area Number |
26293100
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
杉本 真也 東京慈恵会医科大学, 医学部, 講師 (60464393)
田嶌 亜紀子 東京慈恵会医科大学, 医学部, 講師 (70317973)
奥田 賢一 東京慈恵会医科大学, 医学部, 助教 (70624245)
岩瀬 忠行 東京慈恵会医科大学, 医学部, 助手 (80385294)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | バイオフィルム / 黄色ブドウ球菌 / バイオフィルム阻害剤 / Eap / SasG / 細胞壁タンパク質 / 細菌 / 応用微生物 / 感染症 |
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| Outline of Final Research Achievements |
The combined deletion of Eap and SasG reduced biofilm biomass of Staphylococcus aureus, whereas single deletion did not. Combined deletion of Eap and SasG decreased both roughness and thickness. The pathogenicity of ΔEap ΔSasG was significantly decreased. Our findings highlight the relationship between Eap and SasG in S. aureus biofilm formation and pathogenesis. High-throughput screening identified norgestimate (NGM) as an inhibitor of biofilm formation by staphylococcal strains, including MRSA. NGM inhibited the production of extracellular matrix components that are important for biofilm formation, thereby inhibiting biofilm formation by clinical isolates with diverse matrix components. S. aureus caused biofilm dispersal by nuclease production and this depended on environmental pH. Dispersed bacteria showed highly virulence than planktonic bacteria in vitro and in vivo. Dispersed bacteria decreased phagocytosis by PMN and caused a lethal infection in mouse within 24 h.
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Report
(5 results)
Research Products
(104 results)
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[Journal Article] Norgestimate inhibits staphylococcal biofilm formation and resensitizes methicillin-resistant Staphylococcus aureus to β-lactam antibiotics.2017
Author(s)
Yoshii Y, Okuda K, Yamada S, Nagakura M, Sugimoto S, Nagano T, Okabe T, Kojima H, Iwamoto T, Kuwano K, Mizunoe Y.
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Journal Title
NPJ Biofilms Microbiomes.
Volume: 3
Issue: 1
Pages: 18-18
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Immuno-Electron Microscopy of Primary Cell Cultures from GeneticallyModified Animals in Liquid by Atmospheric Scanning Electron Microscopy (ASEM)2014
Author(s)
T.Kinoshita, Y.Mori, K.Hirano, S.Sugimoto, K.Okuda, S.Matsumoto, T.Namiki, T.Ebihara, M.Kawata, H.Nishiyama, M.Sato, M.Suga, K.Higashiyama, K.Sonomoto, Y.Mizunoe, S.Nishihara, C.Sato
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Journal Title
Microscopy and Microanalysis
Volume: 20
Issue: 2
Pages: 469-483
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Norgestimateは黄色ブドウ球菌のバイオフィルム形成を阻害し, βラクタム系抗菌薬に対する黄色ブドウ球菌の感受性を上昇させる.2017
Author(s)
吉井悠, 奥田賢一, 山田聡美, 永倉茉莉, 杉本真也, 長野哲雄, 岡部隆義, 小島宏建, 岩本武夫, 水之江義充.
Organizer
第31回日本バイオフィルム学会
Related Report
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[Presentation] Identification of ABC-JK2, a Small Molecule Inhibitor of Staphylococcal Biofilm Formation.2016
Author(s)
Yoshii Y, Okuda K, Yamada S, Nagakura M, Sugimoto S, Nagano T, Okabe T, Kojima H, Mizunoe Y.
Organizer
2016 ASM Microbe
Place of Presentation
Boston, USA
Year and Date
2016-06-16
Related Report
Int'l Joint Research
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[Presentation] バイオフィルム感染症制圧の試み.2016
Author(s)
水之江 義充, 奥田 賢一, 岩瀬 忠行, 千葉 明生, 杉本 真也.
Organizer
電子情報通信学会 有機エレクトロニクス研究会
Place of Presentation
熊本県熊本市
Year and Date
2016-01-08
Related Report
Invited
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[Presentation] 細菌のバイオフィルムマトリクスの解析.2015
Author(s)
水之江 義充, 千葉 明生, 岩瀬 忠行, 杉本 真也.
Organizer
2015年度電子情報通信学会ソサイエティ大会 界面ナノバイオテクノロジーシンポジウム
Place of Presentation
宮城県仙台市
Year and Date
2015-09-08
Related Report
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