| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
|
|---|
| Outline of Final Research Achievements |
Using knockout (KO) mice we have previously revealed that TMPRSS2, which is expressed on epithelia of the respiratory tract is an essential protease for proteolytic activation of influenza viruses. In TMPRSS2 KO mice the hemagglutinin (HA) of influenza viruses remained to be uncleared and virus replication was severely impaired. In the present study we focused on Sendai virus, a member of the Paramyxoviridae family, because mice are the natural host of Sendai virus and Sendai virus also requires host proteases for its activation in vivo. Sendai virus showed a lethal infection for wild type mice, while TMPRSS2 KO mice were resistant to the Sendai virus infection. The replication of Sendai virus was severely restricted in the TMPRSS2 KO mice. These data for the first time demonstrated that TMPRSS2 is the essential protease for proteolytic activation of an ARI virus, Sendai virus, in the natural host.
|
