Molecular imaging of brain disease model mice by MRI and EPR imaging

• Project/Area Number: 26293280
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Sapporo Medical University
• Principal Investigator: FUJII HIROTADA 札幌医科大学, 医療人育成センター, 教授 (70209013)
• Co-Investigator(Renkei-kenkyūsha): SHIMOHAMA Syun 札幌医科大学, 医学部, 教授 (60235687) ; SATO Shingo 山形大学, 理工学研究科, 教授 (30215793) ; AKABA Hideo 大阪大学, 基礎工学研究科, 准教授 (00552077)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 脳疾患 / 分子イメージング / MRI / EPR / 酸化ストレス / レドックス / イメージング / 脳・神経疾患 / ROS / 磁気共鳴 / ストレス / 脳・神経 / 可視化

Outline of Final Research Achievements

Reactive oxygen species (ROS) and free radicals are continuously generated in brains. An excess of ROS has been reported to play an important role in neurodegenerative diseases. In the present stud, a new magnetic resonance-based imaging method using electron paramagnetic resonance and MRI was develop in order to visualize redox status in neurodegenerative disease model animals. Three animal models such as Alzheimer disease and epilepsy, was employed. Our new molecular imaging method clearly visualized a change in redox status of mouse brain, and these results can be used for medicine.

Research Products

• [Journal Article] Non-invasive imaging of the levels and effects of glutathione on the redox status of mouse brain using electron paramagnetic resonance imaging (2017)
  • Author(s): Miho C. Emoto, Yuta Matsuoka, Ken-ichi Yamada, Hideo Sato-Akaba, Hirotada G. Fujii
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 485 Issue: 4 Pages: 802-806
  • DOI: 10.1016/j.bbrc.2017.02.134
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Brain redox imaging in the pentylenetetrazole (PTZ)-induced kindling model of epilepsy by using in vivo electron paramagnetic resonance and a nitroxide imaging probe. (2015)
  • Author(s): Emoto CM, Yamato M, Sato-Akaba H, Yamada K, Fujii GH.
  • Journal Title: Neuroscience Letters Volume: 608 Pages: 40-44
  • DOI: 10.1016/j.neulet.2015.10.008
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dynamic changes in the distribution and time course of blood-brain barrier-permeative nitroxides in the mouse head with EPR imaging. (2014)
  • Author(s): Emoto MC, Sato-Akaba H, Hirata H, Fujii HG.
  • Journal Title: Free Radical Biology & Medicine Volume: 74 Pages: 222-228
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] In Vivo Brain Redox Status in Diethylmaleate-Treated Mice Using EPR Imaging (2016)
  • Author(s): 3.Hirotada G Fujii, Miho C Emoto, Yuta Matsuoka, Ken-ichi Yamada
  • Organizer: SfRBM's 23rd Annual Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] Development of Theranostics Imaging Probe for MRI and EPR Imaging (2015)
  • Author(s): Emoto, Miho Sato, Shingo Fujii, Hirotada G.
  • Organizer: 23th ISMRM
  • Place of Presentation: Toronto Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research