The attempt of new molecular therapy for esophageal cancer using the analysis of regulation concerning genome and epigenome
| Project/Area Number |
26293298
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井ノ上 逸朗 国立遺伝学研究所, 総合遺伝研究系, 教授 (00192500)
阿久津 泰典 千葉大学, 医学(系)研究科(研究院), 講師 (00375677)
村上 健太郎 千葉大学, 医学部附属病院, 助教 (40436382)
田村 裕 千葉大学, 医学(系)研究科(研究院), 准教授 (50263174)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | 食道癌 / ゲノム / エピゲノム / 分子治療 / 外科 / 癌 / 遺伝子 / トランスレーショナルリサーチ / 発癌制御 / 全ゲノムシーケンス / がん抑制遺伝子 / 遺伝子変異 / 発現制御 |
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| Outline of Final Research Achievements |
Analysis of allele specific expression in esophageal squamous cell carcinoma with combination of exome sequencing and mRNA Sequencing. Exome sequenceing analysis of 25 ESCC cases identified TP53 and ZNF750 as significantly mutated genes. Each gene was analyzed in detail with combination of DNA sequencing data and RNA sequencing. The expression level of TP53 with splicing site mutations, stopgain mutations, and indels were decreased significantly, which was seemed to cause dysfanction of p53 protein. Although expression of TP53 with nonsynchronous mutation was maintained, frequencies of nonsynchronous mutation allele in RNA were increased than those in DNA, which possibly cause dysfanction of its product. Comparing between normal and tumor tissues, expression of VNF750 were decreased, which ZNF750 expression of 2 cases with nonsynchronous mutations were maintained.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Fra-1 Regulates the Expression of HMGA1, Which is Associated with a Poor Prognosis in Human Esophageal Squamous Cell Carcinoma.2016
Author(s)
Toyozumi T, Hoshino I, Takahashi M, Usui A, Akutsu Y, Hanari N, Murakami K, Kano M, Akanuma N, Suitoh H, Matsumoto Y, Sekino N, Komatsu A, Matsubara H.
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Journal Title
Ann Surg Oncol.
Volume: Nov 23.[Epubahead of print]
Related Report
Peer Reviewed
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[Journal Article] Quantification of plasma exosome is a potential prognostic marker for esophageal squamous cell carcinoma.2016
Author(s)
Matsumoto Y, Kano M, Akutsu Y, Hanari N, Hoshino I, Murakami K, Usui A, Suito H, Takahashi M, Otsuka R, Xin H, Komatsu A, Iida K, Matsubara H.
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Journal Title
Oncol Rep.
Volume: 36
Issue: 5
Pages: 2535-2543
DOI
Related Report
Peer Reviewed
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[Journal Article] Heterogeneity of Glucose Metabolism in Esophageal Cancer Measured by Fractal Analysis of Fluorodeoxyglucose Positron Emission Tomography Image: Correlation between Metabolic Heterogeneity and Survival.2016
Author(s)
Tochigi T, Shuto K, Kono T, Ohira G, Tohma T, Gunji H, Hayano K, Narushima K, Fujishiro T, Hanaoka T, Akutsu Y, Okazumi S, Matsubara H.
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Journal Title
Dig Surg.
Volume: Dec 9. [Epub ahead of print]
Related Report
Peer Reviewed
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[Presentation] Analysis of allele specific expression in esophageal squamous cell carcinoma with combination of exome sequencing and mRNA sequencing2017
Author(s)
Masahiko Takahashi, Hirofumi Nakaoka, Naoyuki Hanari, Kentaro Murakam, Masayuki Kano, Yasunori Matsumoto, Ryota Otsuka, Nobufumi Sekino, Masaya Yokoyama, Itsuro Inoue, Hisahiro Matsubara
Organizer
AACR Precision Medicine Series: Opportunities and Challenges of Exploiting Synthetic Lethality in Cancer
Place of Presentation
San Diego, California, USA
Year and Date
2017-01-04
Related Report
Int'l Joint Research
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[Presentation] Usefulness of exosome quantification by cholesteryl ester transfer protein activity as a biomarker for human esophageal squamous cell carcinoma (ESCC)2016
Author(s)
Yasunori Matsumoto, Masayuki Kano, Yasunori Akutsu, Naoyuki Hanari, Isamu Hoshino, Kentaro Murakami, Akihiro Usui, Hiroshi Suito, Masahiko Takahashi, Ryota Otsuka, Hisahiro Matsubara
Organizer
AACR Annual Meeting 2016
Place of Presentation
New Orleans, Louisiana, USA
Year and Date
2016-04-16
Related Report
Int'l Joint Research
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[Presentation] 食道癌治療の新たな展開2014
Author(s)
松原 久裕
Organizer
第1301回千葉医学会例会
Place of Presentation
千葉大学医学部 千葉県・千葉市
Year and Date
2014-11-30
Related Report
Invited
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