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Research on the treatment of esophageal cancer with anti-human fibroblast growth factor receptor like-1

Research Project

Project/Area Number 26293302
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKyoto University

Principal Investigator

SHIMADA Yutaka  京都大学, 薬学研究科(研究院), 教授 (30216072)

Co-Investigator(Kenkyū-buntansha) 清水 一治  京都大学, 薬学研究科(研究院), 教授 (50456836)
武井 義則  京都大学, 薬学研究科(研究院), 講師 (30502455)
Co-Investigator(Renkei-kenkyūsha) ITOU Yuji  鹿児島大学, 理工学研究科, 教授 (60223195)
TUCHIYA Soken  熊本大学, 生命科学研究部, 講師 (80423002)
MARUSAWA Hiroyuki  京都大学, 医学(系)研究科, 講師 (80324630)
OHTSUJI Eigo  京都府立医科大学, 医学(系)研究科, 教授 (20244600)
TSUKADA Kazuhiro  富山大学, 医学薬学研究部(医学), 教授 (90171967)
MATSUMOTO Osamu  千葉科学大学, 薬学部, 教授 (10231599)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords癌 / FGFRL1 / 食道癌 / 抗体治療 / 抗体作成
Outline of Final Research Achievements

We identified fibroblast growth factor receptor like-1 (FGFRL1) as the main prognostic factor among 5 different FGFR receptors. The Duolink method revealed that FGFRL1 formed heterodimers with FGFR1, 3, and 4 following an inoculation with FGF-2. The phage display method showed anti-FGFRL1 antibodies in the blood of esophageal cancer patients. We then newly established an anti-FGFRL1 monoclonal antibody that inhibits (80%) the growth of several esophageal cancer cell lines in vitro.
We established two FGFRL1 knockout cells (KO15 and KO21). These cells reduced actin fragments, resulting in growth suppression not only in vitro, but also in vivo. A histological examination revealed that in contrast to parental cells, FGFRL1 knockout cells exhibited the ability to differentiate. Furthermore, the expression of MMP-1 was markedly suppressed in knockout cells. Our results demonstrate that anti-FGFRL1 treatments represent a promising strategy for esophageal cancer.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Role of fibroblast growth factor receptors in esophageal squamous cell carcinoma2016

    • Author(s)
      Shimada Y, Okumura T, Takei Y, Watanabe K, Nagata T, Hori T, Tsuchiya S, Tsukada K, Shimizu K
    • Journal Title

      Esophagus

      Volume: 13 Issue: 1 Pages: 30-41

    • DOI

      10.1007/s10388-015-0486-4

    • NAID

      40020705045

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] p75 neurotrophin receptor expression is a characteristic of the mitotically quiescent cancer stem cell population present in esophageal squamous cell carcinoma.2016

    • Author(s)
      Yamaguchi T, Okumura T, Hirano K, Watanabe T, Nagata T, Shimada Y, Tsukada K.
    • Journal Title

      Int J Oncol

      Volume: 48 Issue: 5 Pages: 1943-1954

    • DOI

      10.3892/ijo.2016.3432

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Detection of circulating tumor cells by p75NTR expression in patients with esophageal cancer.2016

    • Author(s)
      Yamaguchi T, Okumura T*, Hirano K, Watanabe T, Nagata T, Shimada Y, Tsukada K
    • Journal Title

      World Journal of Surgical Oncology

      Volume: 14 Issue: 1 Pages: 40-47

    • DOI

      10.1186/s12957-016-0793-9

    • NAID

      120005758258

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma2016

    • Author(s)
      Okumura T, Kojima H, Miwa T, Sekine S, Hashimoto I, Hojo S, Nagata T, Shimada Y
    • Journal Title

      World Journal of Surgical Oncology

      Volume: 14 Issue: 1 Pages: 228-228

    • DOI

      10.1186/s12957-016-0985-3

    • NAID

      120005997654

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti cancer effect of Saxifraga stolonifera Meeb2016

    • Author(s)
      T. Nagata, N. N. Win, L. X. Long, T. Miwa, T. Okumura, H. Fushimi, H. Morita, Y. Shimada.
    • Journal Title

      Journal of clinical & experimental pharmacology.

      Volume: 6 Issue: 03 Pages: 1000211-1000211

    • DOI

      10.4172/2161-1459.1000211

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MicroRNA Profiles to Predict Postoperative Prognosis in Patients with Small Cell Carcinoma of the Esophagus2015

    • Author(s)
      Okumura T, Shimada Y, Omura T, Hirano K, Nagata T, Tsukada K
    • Journal Title

      AntiCancer Res

      Volume: 35 Pages: 719-727

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus2015

    • Author(s)
      Okumura T , Shimada Y , Omura T , Hirano K , Nagata T , Tsukada K
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 719-729

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abnormal cell proliferation in the p75NTR-positive basal cell compartment of the esophageal epithelium during squamous carcinogenesis2014

    • Author(s)
      Okumura T, Shimada Y, Sakurai T, Hori R, Nagata T, Sakai Y, Tsukada K
    • Journal Title

      Diseases of the Esophagus

      Volume: 28 Issue: 7 Pages: 634-643

    • DOI

      10.1111/dote.12245

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of fibroblast growth factor receptor-like 1 (FGFRL1) in esophageal squamous cell carcinoma2014

    • Author(s)
      Shimada Y, Okumura T, Nagata T, Hashimoto I, Sawada S, Yoshida T, Fukuoka J, Shimizu K, Tsukada K
    • Journal Title

      Esopagus

      Volume: 11 Issue: 1 Pages: 48-53

    • DOI

      10.1007/s10388-013-0394-4

    • NAID

      40020229726

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MicroRNA-203 inhibits the progression of esophageal squamous cell carcinoma with restored epithelial tissue architecture in vivo.2014

    • Author(s)
      Okumura T, Shimada Y, Moriyama M, Takei Y, Omura T, Sekine S, Nagata T, Shimizu K, Tsukada K.
    • Journal Title

      Int J Oncol

      Volume: 44 Issue: 6 Pages: 1923-1932

    • DOI

      10.3892/ijo.2014.2365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Circulating microRNA expression profiles as a novel diagnostic biomarker for esophageal squamous cell carcinoma2017

    • Author(s)
      Shimada Y, Takei Y, Okumura T, Nagata T, Fujinami H, Arima M, Abe T, Niwa Y, Tajika M, Sudo T, Shimizu K
    • Organizer
      108th Annual Meeting of the American Association for Cancer Research
    • Place of Presentation
      Washington, DC
    • Year and Date
      2017-03-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Blood supply visualization for reconstruction during esophagectomy2015

    • Author(s)
      Shimada Y, Okumura T, Moriyama M, Nagata T, Matsui K, Osada R, Tsukada K
    • Organizer
      46th World Congress of Surgery
    • Place of Presentation
      Bangkok
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of an esophageal small cell carcinoma cell line (TYUC-1)2015

    • Author(s)
      Shimada Y, Okumura T, Takei Y, Watanabe K, Nagata T, Tsukada K, Yamane A, Nishiyama M, Hirasawa A, Shimizu K
    • Organizer
      2015 annual meeting of American Association of Cancer Research
    • Place of Presentation
      Philadelphia
    • Year and Date
      2015-04-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of finbroblast growth factor receptors in esophageal squamous cell carcinoma2014

    • Author(s)
      Shimada Y, Okumura T, Nagata T, Tsukada K, Shimizu K.
    • Organizer
      2014 annual meeting of American Association of Cancer Research
    • Place of Presentation
      San Diego (USA)
    • Year and Date
      2014-04-05 – 2014-04-09
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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