| Project/Area Number |
26303011
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 海外学術 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
Sano Daisuke 北海道大学, 工学(系)研究科(研究院), 准教授 (80550368)
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| Co-Investigator(Kenkyū-buntansha) |
大村 達夫 東北大学, 学内共同利用施設等, 教授 (30111248)
片山 浩之 東京大学, 工学(系)研究科(研究院), 准教授 (00302779)
石井 聡 北海道大学, 工学(系)研究科(研究院), 助教 (10612674)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 病原体 / 下水 / スペイン / 病原ウイルス / 病原細菌 / 輸入感染症 / 食中毒 |
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| Outline of Final Research Achievements |
This study aims to identify pathogens in wastewater collected in Spain. Firstly, a simultaneous detection method of pathogenic viruses and bacteria was developed. Wastewater samples and fecal samples from gastroenteritis patients were collected in Spain, and applied to the newly developed method. As a result, norovirus GII was found to be the most important infectious agent in wastewater. Norovirus genome sequences were then analyzed by next-generation sequencing, and its nucleotide diversity was evaluated. Also, primer and probe sets for separately quantifying multiple genotypes of norovirus were designed and validated.
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