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Identification of pathogens in sewage collected in Spain in order to predict imported infectious agents in Japan

Research Project

Project/Area Number 26303011
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Civil and environmental engineering
Research InstitutionHokkaido University

Principal Investigator

Sano Daisuke  北海道大学, 工学(系)研究科(研究院), 准教授 (80550368)

Co-Investigator(Kenkyū-buntansha) 大村 達夫  東北大学, 学内共同利用施設等, 教授 (30111248)
片山 浩之  東京大学, 工学(系)研究科(研究院), 准教授 (00302779)
石井 聡  北海道大学, 工学(系)研究科(研究院), 助教 (10612674)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywords病原体 / 下水 / スペイン / 病原ウイルス / 病原細菌 / 輸入感染症 / 食中毒
Outline of Final Research Achievements

This study aims to identify pathogens in wastewater collected in Spain. Firstly, a simultaneous detection method of pathogenic viruses and bacteria was developed. Wastewater samples and fecal samples from gastroenteritis patients were collected in Spain, and applied to the newly developed method. As a result, norovirus GII was found to be the most important infectious agent in wastewater. Norovirus genome sequences were then analyzed by next-generation sequencing, and its nucleotide diversity was evaluated. Also, primer and probe sets for separately quantifying multiple genotypes of norovirus were designed and validated.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (17 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results) Book (1 results) Funded Workshop (1 results)

  • [Int'l Joint Research] バルセロナ大学/サンティアゴ・デ・コンポステーラ大学(スペイン)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] バルセロナ大学/生物学部/微生物学科(スペイン)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Estimation of concentration ratio of indicator to pathogen-related gene in environmental water based on left-ceonsored data2016

    • Author(s)
      Tsuyoshi Kato, Ayano Kobayashi, Toshihiro Ito, Takayuki Miura, Satoshi Ishii, Satoshi Okabe and Daisuke Sano
    • Journal Title

      Journal of Water and Health

      Volume: 14 Issue: 1 Pages: 14-25

    • DOI

      10.2166/wh.2015.029

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Bactericidal and virucidal mechanisms in the alkaline treatment of a compost using calsium lime and ash2016

    • Author(s)
      Nowaki Hijikata, Rui Tezuka, Shinobu Kazama, Masahiro Otaki, Ken Ushijima, Ryusei Ito, Satoshi Okabe, Daisuke Sano, Naoyuki Funamizu
    • Journal Title

      Journal of Environmental Management

      Volume: 181 Pages: 721-727

    • DOI

      10.1016/j.jenvman.2016.08.026

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular epidemiology of noroviruses detected in Nepalese children with acute diarrhea between 2005 and 2011: increase and predominance of minor genotype GII.132015

    • Author(s)
      Thi Nguyen Hoa-Trana, Toyoko Nakagomi, Daisuke Sano, Jeevan B. Sherchand, Basu D. Pandey, Nigel A. Cunliffe, Osamu Nakagomi
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 30 Pages: 27-36

    • DOI

      10.1016/j.meegid.2014.12.003

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Water quality monitoring and risk assessment by simultaneous multi-pathogen quantification2014

    • Author(s)
      Satoshi Ishii, Takamitsu Nakamura, Shuji Ozawa, Ayano Kobayashi, Daisuke Sano, Satoshi Okabe
    • Journal Title

      Environmental Science and Technology

      Volume: 48(9) Issue: 9 Pages: 4744-4749

    • DOI

      10.1021/es500578s

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 水環境におけるノロウイルスの遺伝子型別存在実態の把握2017

    • Author(s)
      宮村明帆、北島正章、岡部聡、佐野大輔
    • Organizer
      第51回日本水環境学会年会
    • Place of Presentation
      熊本大学(熊本市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ベイズモデルを用いた下水処理過程における病原ウイルス除去効率の定量評価2015

    • Author(s)
      伊藤寿宏
    • Organizer
      日本水環境学会・水中の健康関連微生物研究委員会シンポジウム「水中の病原微生物によるリスク制御への総合対策に向けて」
    • Place of Presentation
      発明会館(東京都港区)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微小ナノ流路デバイスを用いたDHS(Downflow Hanging Sponge)リアクターにおけるヒト感染性ウイルスの定量評価2015

    • Author(s)
      小林直央
    • Organizer
      日本水環境学会・水中の健康関連微生物研究委員会シンポジウム「水中の病原微生物によるリスク制御への総合対策に向けて」
    • Place of Presentation
      発明会館(東京都港区)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Estimation of pathogen density distribution in water based on left-cencored dataset: Bayesian vs Maximum a posteriori2015

    • Author(s)
      Daisuke Sano
    • Organizer
      International Environmental Engineering Conference 2015
    • Place of Presentation
      BEXCO(Busan, Korea)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection of norovirus in sewage by quenching probe based loop-mediated isothermal amplification (Q-LAMP) method2015

    • Author(s)
      Yo Ueki
    • Organizer
      18th International Symposium on Health-Related Water Microbiology
    • Place of Presentation
      リスボン大学(Lisbon, Portugal)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下水再生利用に関わる下水処理装置の性能評価及び運転管理手法の提案2015

    • Author(s)
      佐野大輔
    • Organizer
      環境工学委員会・次世代下水道小委員会講演会「次世代の下水道における衛生的課題とその対応」
    • Place of Presentation
      北海道大学・フロンティア応用科学研究棟(北海道札幌市)
    • Year and Date
      2015-08-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Bayesian estimation of virus removal efficiency in wastewater treatment plant based on left-censored dataset2015

    • Author(s)
      Toshihiro Ito
    • Organizer
      Water and Environment Technology Conference 2015
    • Place of Presentation
      日本大学(東京都千代田区)
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of virus removal efficiency in a pilot-scale MBR with microfluidic quantitative PCR2015

    • Author(s)
      Toshihiro Ito
    • Organizer
      WEF-EESS Asia-Pacific Wastewater Treatment and Reuse Conference 2015
    • Place of Presentation
      シンガポール国立大学(Singapore)
    • Year and Date
      2015-06-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic variations of norovirus GII.4 within a single host and among hosts2015

    • Author(s)
      Daisuke Sano
    • Organizer
      Special Workshop on Health-Related Environmental Virology
    • Place of Presentation
      バルセロナ大学(Barcelona, Spain)
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic Dynamic Arrayを用いたパイロットスケールMBRプラントにおける複数種病原ウイルスの同時定量2015

    • Author(s)
      伊藤寿宏
    • Organizer
      第2回環境水質工学シンポジウム
    • Place of Presentation
      山形大学農学部(山形県鶴岡市)
    • Year and Date
      2015-05-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 左側打切りデータに対するMAP推定の適用2015

    • Author(s)
      加藤毅、岡部聡、佐野大輔
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ハイブリダイゼーション法を用いた下水中のウイルスゲノム回収手法の開発2015

    • Author(s)
      勝又雅博,真砂佳史,原田秀樹,大村達夫
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 下水中のポリA鎖を有するウイルスゲノムの選択的増幅手法の検討2015

    • Author(s)
      風間しのぶ,真砂佳史,大村達夫
    • Organizer
      第49回日本水環境学会年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Bayesian modeling of virus removal efficiency in wastewater treatment processes2014

    • Author(s)
      Tsuyoshi Kato, Satoshi Okabe and Daisuke Sano
    • Organizer
      IWA World Water Congress 2014
    • Place of Presentation
      Lisboa Congress Centre, Lisbon, Portugal
    • Year and Date
      2014-09-21 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 異なる測定限界における切断分布のベイズ推定の適用2014

    • Author(s)
      加藤毅、岡部聡、佐野大輔
    • Organizer
      第17回日本水環境学会シンポジウム
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2014-09-08 – 2014-09-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Estimation of quantitative relationships between pathogens and indicator microorganisms in water using left-censored data2014

    • Author(s)
      Daisuke Sano, Tsuyoshi Kato, Ayano Kobayashi, Toshihiro Ito, Satoshi Ishii and Satoshi Okabe
    • Organizer
      4th Food and Environmental Virology Conference
    • Place of Presentation
      Ionian Academy, Corfu town, Greece
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Quantification of multiple enteric viruses from environmental water samples by using microfluidic quantitative PCR2014

    • Author(s)
      Satoshi Ishii, Gaku Kitamura, Ayano Kobayashi, Takayuki Miura, Daisuke Sano and Satoshi Okabe
    • Organizer
      ASM2014
    • Place of Presentation
      Boston Convention & Exhibition Center, Boston, MA, USA
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Annual Research Report
  • [Book] 第4章 ウイルス 2各論 4ウイルス別検査法(8)糞便系ファージ 食品衛生検査指針 微生物編2015

    • Author(s)
      片山浩之
    • Publisher
      公益社団法人日本食品衛生協会
    • Related Report
      2014 Annual Research Report
  • [Funded Workshop] 第5回国際食品・環境ウイルス学会2016

    • Place of Presentation
      ホテル櫻井(群馬県草津町)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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