Construction of the libraries and the database including health functions in Nepalese wild mushrooms

• Project/Area Number: 26304047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: Kyushu University
• Principal Investigator: Shimizu Kuniyoshi 九州大学, 農学研究院, 准教授 (20346836)
• Co-Investigator(Kenkyū-buntansha): 土居 克実 九州大学, 農学研究院, 教授 (40253520) ; 片倉 喜範 九州大学, 農学研究院, 准教授 (50264106)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: キノコ / 生理活性 / 機能性成分 / ネパール / 健康食品 / 化粧品 / Inonotus / hisidin / hispidin / 機能性食品 / 遺伝子資源 / Ganoderma / 微生物 / 菌類 / 生物多様性 / 抗アレルギー / 抗菌活性

Outline of Final Research Achievements

In this study, we collected over 150 species of wild mushrooms in Nepal. We examined its antioxidant activity, melanin production inhibitory (promoting) activity, antiallergic activity and antibacterial activity. As a result, we found promising mushrooms showing several high functionality. In particular, the extract of Inonotus clemensiae showed high antioxidant activity, antiallergic activity and antibacterial activity. We succeeded in isolating its active ingredient Hispidin. It is also highly stable in the body. The findings obtained in this study, including species data showing similarly high activity, will be the basic knowledge for advanced use of Nepalese mushrooms.

Research Products

• [Int'l Joint Research] Nepal Agricultural Research Council(Nepal)
• [Int'l Joint Research] Nepal Agricultural Research Council(Nepal)
• [Int'l Joint Research] Nepal Agriculture Research Council(Nepal)
• [Journal Article] Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes (2017)
  • Author(s): S. Tamrakar, M. Nishida, Y. Amen, H. B. Tran, H. Suhara, K. Fukami, G. P. Parajuli, K. Shimizu
  • Journal Title: Journal of Wood Science Volume: 63 Issue: 4 Pages: 379-387
  • DOI: 10.1007/s10086-017-1636-1
  • NAID: 40021280413
  • Peer Reviewed
• [Journal Article] Nepal Mushroom Project (2017)
  • Author(s): Sonam Tamrakar, Katsuya Fukami, Kuniyoshi Shimizu
  • Journal Title: アグリバイオ Volume: 1 Pages: 484-486
  • NAID: 40021807152
• [Journal Article] Nepal Mushroom Project (2017)
  • Author(s): Sonam Tamrakar, Katsuya Fukami, Kuniyoshi Shimizu
  • Journal Title: アレルギーの臨床 Volume: 37 Pages: 889-891
  • NAID: 40021807152
• [Journal Article] Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes (2017)
  • Author(s): Sonam Tamrakar, Marina Nishida, Yhiya Amen, Hai Bang Tran, Hiroto Suhara, Katsuya Fukami, Gopal Prasad Parajuli, Kuniyoshi Shimizu
  • Journal Title: Journal of Wood Science Volume: 印刷中
  • NAID: 40021280413
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species (2016)
  • Author(s): Tamrakar, S., Tran, H.B., Nishida, M., Kaifuchi, S., Suhara, H., Doi, K., Fukami, K., Parajuli, G.P., Shimizu, K.
  • Journal Title: Journal of Natural Medicines Volume: 70 Issue: 4 Pages: 769-779
  • DOI: 10.1007/s11418-016-1013-1
  • NAID: 40020934105
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Antioxidant Activities of 62 Wild Mushrooms from the forests of Nepal and their phenolic profile by HPLC analysis (2016)
  • Author(s): Sonam Tamrakar, Hai Bang Tran, Marina Nishida, Satoru Kaifuchi, Hiroto Suhara, Katsumi Doi, Katsuya Fukami, Gopal Prasad Parajuli, Kuniyoshi Shimizu
  • Journal Title: Journal of Natural Medicine Volume: 未確定
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Wild mushrooms in Nepal : some potential candidates as antioxidant and ACE-inhibition sources. (2014)
  • Author(s): Hai Bang T, Suhara H, Doi K, Ishikawa H, Fukami K, Parajuli GP, Katakura Y, Yamashita S. Watanabe K, Adhikari MK, Manandhar HK, Kondo R, Shimizu K.
  • Journal Title: Evidence-based Complementary and Alternative Medicine Volume: 2014 Issue: 1 Pages: 195305-195305
  • DOI: 10.1155/2014/195305
  • Peer Reviewed / Open Access
• [Presentation] Nepalese Wild Mushrooms: A Treasure Trove of Various Biological Activities (2016)
  • Author(s): Sonam Tamrakar, Hai Bang Tran, Marina Nishida, Satoru Kaifuchi, Hiroto Suhara, Katsumi Doi, Katsuya Fukami, Gopal Prasad Parajuli, Kuniyoshi Shimizu
  • Organizer: 19th International Society for Mushroom Science (ISMS) Congress
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2016-05-29
  • Int'l Joint Research
• [Presentation] Bioactivity and Chemical Characterization of some Wild Mushrooms of Nepal (2016)
  • Author(s): Sonam TAMRAKAR, Hai Bang TRAN, Marina NISHIDA, Hiroto SUHARA, Katsumi DOI, Katsuya FUKAMI, Gopal Prasad PARAJULI, Kuniyoshi SHIMIZU
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2016-03-27
• [Presentation] Investigation of the Antioxidant Activities of the Wild Mushrooms of Nepal (2015)
  • Author(s): TAMRAKAR, Marina NISHIDA, Hiroto SUHARA*, Katsumi DOI, Katsuya FUKAMI, Gopal Prasad PARAJULI, Kazuo WATANABE, Mahesh Kumar ADHIKARI, Hira Kaji MANANDHAR, Kuniyoshi SHIMIZU
  • Organizer: 第65回日本木材学会大会（2015年，東京）
  • Place of Presentation: ワーホール船橋
  • Year and Date: 2015-03-16 – 2015-03-19
• [Presentation] ネパール原産きのこ抽出物がメラニン生合成に与える影響 (2014)
  • Author(s): （九大院生資環）西田麻理奈、Tran Hai Bang, (宮崎県木材利用技術センター）須原弘登、（九大院農）土居克実、（九大有体物管理センター）深見克哉、（NARC) Gopal Prasad Parajuli, 渡辺和夫、Mahesh Kumar Adhikari, Hira Kaji Manandhar, (九大院農）清水邦義
  • Organizer: 日本きのこ学会第１８回大会
  • Place of Presentation: 京都大学 百周年時計台記念館（京都）
  • Year and Date: 2014-09-10 – 2014-09-12
• [Presentation] ネパール原産キノコエタノール抽出物の生理活性評価 (2014)
  • Author(s): （九大院生資環）西田麻理奈、Tran Hai Bang, (宮崎県木材利用技術センター）須原弘登、（九大院農）土居克実、（九大有体物管理センター）深見克哉、（NARC) Gopal Prasad Parajuli, 渡辺和夫、Mahesh Kumar Adhikari, Hira Kaji Manandhar, (九大院農）清水邦義
  • Organizer: 第58回日本菌学会
  • Place of Presentation: サイエンスヒルズこまつ（石川県）
  • Year and Date: 2014-06-14 – 2014-06-15
• [Remarks] 森林圏環境資源科学研究室ホームページ
  • URL: http://ffpsc.agr.kyushu-u.ac.jp/sffps/results.html
• [Remarks] 研究業績
  • URL: http://ffpsc.agr.kyushu-u.ac.jp/sffps/shimizu_result.html#genchoronbun
• [Patent(Industrial Property Rights)] メラニン生成制御剤 (2014)
  • Inventor(s): 清水邦義、土居克実
  • Industrial Property Rights Holder: 九州大学、NARC
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-169034
  • Filing Date: 2014-08-22