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The elucidation of mechanisms of the cell death in SSCs induced by radiation

Research Project

Project/Area Number 26340021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionKyoto University

Principal Investigator

Morimoto hiroko  京都大学, 医学研究科, 特定研究員 (90540097)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords精子幹細胞 / 活性酸素 / 放射線 / 細胞死 / p53
Outline of Final Research Achievements

Exposure to ionizing radiation is known to have a strong influence H2O in vivo and induces reactive oxygen species (ROS). ROS makes severe DNA damages. In our study, we identified two molecules involved in the resistance to radiation in spermatogonial stem cells(SSCs). In consequence, we found that Nox3 and Bcl6b are novel molecules involved in the self renewal of SSCs. Activation of Mapk14 or Mapk7 increased the expression of Nox1 and ROS producing. Transplantation of Mapk14 or Mapk7 knocking out SSCs to donor testis resulted in a decrease in the number of colonies of SSCs. These results reveal that MAPK14-MAPK7-BCL6B pathway plays a crucial role in the self renewal of SSCs associated with ROS producing.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 5 results,  Open Access: 5 results)

  • [Journal Article] Fertility of male germline stem cells following spermatogonial transplantation in infertile mouse models.2016

    • Author(s)
      Kanatsu-Shinohara M., Morimoto H., Shinohara T.
    • Journal Title

      Biol Reprod.

      Volume: 94(5) Issue: 5 Pages: 112-112

    • DOI

      10.1095/biolreprod.115.137869

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal.2016

    • Author(s)
      Kanatsu-Shinohara M., Tanaka T., Ogonuki N., Ogura A., Morimoto H., Cheng PF., Eisenman RN., Trumpp A., Shinohara T.
    • Journal Title

      Genes Dev.

      Volume: 30(23) Issue: 23 Pages: 2637-2648

    • DOI

      10.1101/gad.287045.116

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Enrichment of Mouse Spermatogonial Stem Cells by the Stem Cell Dye CDy1.2015

    • Author(s)
      Kanatsu-Shinohara M, Morimoto H, Shinohara T.
    • Journal Title

      Biology of reproduction

      Volume: 94 (1) 13 Issue: 1 Pages: 1-10

    • DOI

      10.1095/biolreprod.115.135707

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells2015

    • Author(s)
      Morimoto H, Kanatsu-Shinohara M, Shinohara T.
    • Journal Title

      Biology of reproduction

      Volume: 92 (6) 147 Issue: 6 Pages: 1-10

    • DOI

      10.1095/biolreprod.114.127647

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal.2015

    • Author(s)
      Takashima S, Kanatsu-Shinohara M, Tanaka T, Morimoto H, Inoue K, Ogonuki N, Jijiwa M, Takahashi M, Ogura A, Shinohara T.
    • Journal Title

      Stem Cell Reports.

      Volume: 4 Issue: 3 Pages: 489-502

    • DOI

      10.1016/j.stemcr.2015.01.010

    • NAID

      120005540400

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improved serum- and feeder-free culture of mouse germline stem cells.2014

    • Author(s)
      Kanatsu-Shinohara M, Ogonuki N, Matoba S, Morimoto H, Ogura A, Shinohara T.
    • Journal Title

      Biol Reprod.

      Volume: 91 Issue: 4 Pages: 88-88

    • DOI

      10.1095/biolreprod.114.122317

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Trp53-Trp53inp1-Tnfrsf10b Pathway Regulates the Radiation Response of Mouse Spermatogonial Stem Cells.2014

    • Author(s)
      Kei Ishii, Masamichi Ishiai, Hiroko Morimoto, Mito Kanatsu-Shinohara, Ohtsura Niwa, Minoru Takata, and Takashi Shinohara.
    • Journal Title

      Stem Cell Reports.

      Volume: 3 Issue: 4 Pages: 676-89

    • DOI

      10.1016/j.stemcr.2014.08.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

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Published: 2014-04-04   Modified: 2018-03-22  

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