| Project/Area Number |
26350131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Eating habits
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| Research Institution | Kanagawa Institute of Technology |
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Principal Investigator |
Kiyose Chikako 神奈川工科大学, 応用バイオ科学部, 教授 (50272745)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 食品成分 / ビタミンＥ / インスリン抵抗性改善 / C2C12細胞 / KKAyマウス / δートコフェロール / ビタミン / 培養細胞 / 動物実験 |
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| Outline of Final Research Achievements |
The aim of this study is the search of food component which improve insulin resistance. First, I tried a construction of insulin resistance cell using C2C12 cells, skeletal muscle cell of mouse. Consequently, I succeeded in the construction of the insulin resistance cell using palmitate. Next, I investigated the effects of vitamin E analogs on the improvement of insulin resistance in these insulin resistance cells. As the results of experiment, I found that delta-tocopherol can improve the phosphorylation of Akt in insulin resistance cells. Secondly, I investigated the effects of delta-tocopherol on the insulin resistance in KKAy mice, a diabetes model mice. The expressions of IRS-1 and IRS-2 mRNA in liver of H group were significantly lower than those of C group. But, there were no significant differences in the expressions of IRS-1 and IRS-2 mRNA between H group and delta-Toc group. Therefore, delta-tocopherol could not show an improvement effect of insulin resistance in KKAy mice.
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