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Clarification of molecular mechanisms underlying the analgesic effect of botulinum toxin and applied study

Research Project

Project/Area Number 26350499
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOkayama University

Principal Investigator

YAMAMOTO YUMIKO  岡山大学, 医歯薬学総合研究科, 助教 (20403496)

Co-Investigator(Kenkyū-buntansha) 松香 芳三  徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (90243477)
小出 隆規  早稲田大学, 理工学術院, 教授 (70322253)
丸濱 功太郎  岡山大学, 医歯薬学総合研究科, 助教 (60712792)
Co-Investigator(Renkei-kenkyūsha) MATSUSHITA Osamu  岡山大学, 大学院医歯薬学総合研究科, 教授 (00209537)
Research Collaborator OGUMA Keiji  岡山大学, 名誉教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsボツリヌス神経毒素 / 神経障害性疼痛 / 疼痛抑制 / SNAP-25 / 細菌
Outline of Final Research Achievements

We developed an assay to measure the proteolytic activity of botulinum toxin (BoNT), and attempted to synthesize fluorescently labeled full-length BoNT, in order to study molecular mechanisms for the analgesic effect of BoNT. We also generated four kinds of anti-peptide antibodies against SNAP-25, and showed that BoNT/A or BoNT/E cleaves SNAP-25 in cultured trigeminal ganglion neurons. This suggests that peripherally administered BoNT decreases the release of neurotransmitters via the proteolysis of SNARE proteins in sensory ganglion neurons and relieves neuropathic pain.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Cross-Excitation in Peripheral Sensory Ganglia Associated with Pain Transmission.2015

    • Author(s)
      Omoto K, Maruhama K, Terayama R, Yamamoto Y, Matsushita O, Sugimoto T, Oguma K, Matsuka Y
    • Journal Title

      Toxins (Basel)

      Volume: 7 Issue: 8 Pages: 2906-2917

    • DOI

      10.3390/toxins7082906

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ボツリヌス毒素を利用した神経障害性疼痛治療2015

    • Author(s)
      松香芳三,丸濱功太郎,山本由弥子,小熊惠二,松下治
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 三叉神経におけるA型ボツリヌス神経毒素の局在と軸索輸送2014

    • Author(s)
      山本由弥子,丸濱功太郎,松香芳三,美間健彦,後藤和義,横田憲治,松下治,小熊惠二
    • Organizer
      第67回日本細菌学会中国・四国支部総会
    • Place of Presentation
      徳島
    • Year and Date
      2014-10-04 – 2014-10-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 精製A型ボツリヌス毒素の軸索輸送と疼痛抑制効果2014

    • Author(s)
      丸濱功太郎,松香芳三,山本由弥子,寺山隆司,杉本朋貞
    • Organizer
      第37回日本神経科学大会 Neuroscience 2014
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Book] 人獣共通感染症 改訂3版 (ボツリヌス症)2016

    • Author(s)
      小熊惠二、山本由弥子、鈴木智典
    • Total Pages
      550
    • Publisher
      医薬ジャーナル社
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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