Novel DNA vaccine strategy to elicit antitumor immune responses by transfecting with plasmids coding for pathogenic antigens
| Project/Area Number |
26350533
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Osaka Prefecture University (2016)
Meiji Pharmaceutical University (2014-2015) |
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Principal Investigator |
Ito Tomoko 大阪府立大学, 生命環境科学研究科, 客員研究員 (80372910)
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| Co-Investigator(Renkei-kenkyūsha) |
KOYAMA Yoshiyuki 大阪府立大学, 生命環境科学研究科, 客員研究員 (00162090)
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| Research Collaborator |
ERIGUCHI Masazumi
USHIGUSA Takahiro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | DNAワクチン / 遺伝子治療 / ガン免疫治療 |
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| Outline of Final Research Achievements |
We have developed a novel antitumor strategy by transfecting with the plasmids coding for pathogenic antigens. We examined the therapeutic effects of the transfection of the plasmid coding a membrane-associating protein, ESAT-6, or a secretory antigen, Ag85B. Both plasmids showed similar significant effects in tumor-bearing mice. Accumulation of T-cells and monocytic cells was observed in the diminished tumors. The antigens synthesized in the tumor cells would be degraded as heterologous proteins, and the degraded peptides would be presented on the cell surfaces as "artificial neoepitopes". The exosomes presenting the epitopes would be secreted from the cells, and were thought to play an essential role in eliciting antitumor immunity. This hypothesis was confirmed by the experiments with the exosomes presenting the "artificial neoepitopes", which had been prepared by transfection of the cultured cells with the pathogenic antigen genes.
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Report
(4 results)
Research Products
(16 results)
