Analysis of trans-plasma membrane molecular machinery between collagen types XV/XVIII and mitochondria

• Project/Area Number: 26350892
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Okayama University
• Principal Investigator: Momota Ryusuke 岡山大学, 医歯薬学総合研究科, 助教 (80263557)
• Co-Investigator(Kenkyū-buntansha): 大塚 愛二 岡山大学, 医歯薬学総合研究科, 教授 (50168986) ; 内藤 一郎 岡山大学, 医学部, 客員研究員 (60362993)
• Co-Investigator(Renkei-kenkyūsha): OOHASHI Toshitaka 岡山大学, 医歯薬学総合研究科, 教授 (50194262) ; YONEZAWA Tomoko 岡山大学, 医歯薬学総合研究科, 助教 (30304299)
• Research Collaborator: KOMIYAMA Takaaki ; NARASAKI Masahiro
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: XV/XVIII型コラーゲン / ミトコンドリア / 細胞骨格 / 診断法 / 基底膜 / コラーゲン / ショウジョウバエ / アンギオテンシンII受容体1型 / チュブリン / アクチン / 細胞内骨格

Outline of Final Research Achievements

To identify the molecules which would compose "the tran-plasma membrane molecular machinery" of collagen types XV/XVIII and mitochondria, we have screened thousands of genes expressed in muscular tissues and identified possible candidate G-protein coupled receptors. In addition, our histological analyses on collagen types XV/XVIII mutant animals revealed abnormal structures of cytoskeletons, which would potentially compromise the mitochondrial and cellular functions. Moreover, we had successfully optimized the immunohistochemical conditions for the anti-collagen XV/XVIII monoclonal antibodies, which enabled us to use variously processed tissue samples to diagnose human diseases caused by defective collagen XV/XVIII.

Research Products

• [Presentation] パブリックの遺伝情報データベースを用いた遺伝子発現の解析 (2016)
  • Author(s): 百田龍輔、大橋俊孝、米澤朋子
  • Organizer: 第34回日本美容皮膚科学会総会・学術大会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Invited
• [Presentation] Characterization of a Drosophila multiplexin lethal mutation and its effects on gene expression (2015)
  • Author(s): Ryusuke Momota, Masahiro Narasaki, Ichiro Naito, Yoshifumi Ninomiya, Aiji Ohtsuka
  • Organizer: The 47th Annual Meeting of JSCRT
  • Place of Presentation: Shikagawa, Japan
  • Year and Date: 2015-05-15 – 2015-05-16
• [Presentation] Characterization of a Drosophila multiplexin lethal mutation and its effect on gene expression (2015)
  • Author(s): Ryusuke Momota, Masahiro Narasaki, Ichiro Naito, Yoshifumi Ninomiya, Aiji Ohtsuka
  • Organizer: 第47回日本結合組織学会学術大会
  • Place of Presentation: 東京コクヨホール
  • Year and Date: 2015-05-15
• [Presentation] Characterization of Drosophila multiplexin lethal allele and its phenotype of steroid hormone deficiency (2014)
  • Author(s): Ryusuke Momota, Masahiro Narasaki, Ichiro Naito, Yoshifumi Ninomiya, Aiji Ohtsuka
  • Organizer: The 46th Annual Meeting of JSCTR
  • Place of Presentation: Wink Aichi, Nagoya, Japan
  • Year and Date: 2014-06-05 – 2014-06-07